BMRBj - BMREntryMaster

Found 1 Document (1.64 seconds)

BMRB: 34172

5OR5

NMR structure of the complex formed by an engineered region 2 of sigmaE in complex with GTAAAA

Authors

Campagne, S., Vorholt, J.A., Allain, F.H.

Assembly

ECF RNA polymerase sigma-E factor,ECF RNA polymerase sigma factor SigW,ECF RNA polymerase sigma-E factor/DNA Complex

Entity

1. ECF RNA polymerase sigma-E factor,ECF RNA polymerase sigma factor SigW,ECF RNA polymerase sigma-E factor/DNA Complex, entity 1 (polymer, Thiol state: not present), 95 monomers, 10975.47 Da Detail

MSEQLTDQVL VERVQKGDQK AFNLLVVRYQ HKVASLVSRY VPSGDVPDVV QEAFIKAYRA LDSFDINRKF STWLYRIAVN TAKNYLVAQG RRLEL

2. ECF RNA polymerase sigma-E factor,ECF RNA polymerase sigma factor SigW,ECF RNA polymerase sigma-E factor/DNA Complex, entity 2 (polymer, Thiol state: not present), 6 monomers, 1824.261 Da Detail

XTAAAA

Total weight

12799.73 Da

Max. entity weight

10975.47 Da

Source organism

Escherichia coli K-12 , Bacillus subtilis subsp. subtilis str. 168 , synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 92.1 %, Completeness (bb): 94.3 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	92.1 % (1103 of 1197)	93.5 % (602 of 644)	89.3 % (400 of 448)	96.2 % (101 of 105)
Backbone	94.3 % (567 of 601)	95.6 % (216 of 226)	92.9 % (262 of 282)	95.7 % (89 of 93)
Sidechain	90.7 % (624 of 688)	92.3 % (386 of 418)	87.6 % (226 of 258)	100.0 % (12 of 12)
Aromatic	79.2 % (84 of 106)	93.1 % (54 of 58)	61.7 % (29 of 47)	100.0 % (1 of 1)
Methyl	93.7 % (119 of 127)	95.3 % (61 of 64)	92.1 % (58 of 63)

1. entity 1

MSEQLTDQVL VERVQKGDQK AFNLLVVRYQ HKVASLVSRY VPSGDVPDVV QEAFIKAYRA LDSFDINRKF STWLYRIAVN TAKNYLVAQG RRLEL

2. entity 2

XTAAAA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] Engineered region2 of sigmaE from E. coli in which the loop L3 was replaced by the loop L3 of Bacillus subtilis sigmaW, 1 mM DNA (5'-D(*GP*TP*AP*AP*AP*A)-3'), 10 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O.

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
2	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
3	sodium chloride	natural abundance	50 mM
4	sodium phosphate	natural abundance	10 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] Engineered region2 of sigmaE from E. coli in which the loop L3 was replaced by the loop L3 of Bacillus subtilis sigmaW, 1 mM DNA (5'-D(*GP*TP*AP*AP*AP*A)-3'), 10 mM sodium phosphate, 50 mM sodium chloride, 100% D2O.

#	Name	Isotope labeling	Concentration
5	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
6	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
7	sodium chloride	natural abundance	50 mM
8	sodium phosphate	natural abundance	10 mM

Release date

2017-08-16

Citation 1

Structural basis for -10 promoter element melting by environmentally induced sigma factors
Campagne, S., Marsh, M.E., Capitani, G., Vorholt, J.A., Allain, F.H.
Nat. Struct. Mol. Biol. (2014), 21, 269-276, PubMed 24531660 , DOI 10.1038/nsmb.2777 , Abstract

Show more 1 citaion

Citation 2

Engineered promoter selectivity of an ECF sigma factor
Campagne, S., Vorholt, J.A., Allain, F.H., Campagne, S., Marsh, M.E., Capitani, G., Vorholt, J.A., Allain, F.H.
J. Mol. Biol.

Hide non-primary 1 citaion

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 19366 released on 2014-02-09
    Title NMR structure of region 2 of E. coli sigmaE
  BMRB: 19367 released on 2014-02-09
    Title Solution structure of the complex formed by the region 2 of E. coli sigmaE and its cognate -10 non template element TGTCAAA

Related entities 1. ECF RNA polymerase sigma-E factor,ECF RNA polymerase sigma factor SigW,ECF RNA polymerase sigma-E factor/DNA Complex, entity 1, : 1 : 24 entities Detail

Experiments performed 13 experiments Detail

1 3D HNCACB

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] Engineered region2 of sigmaE from E. coli in which the loop L3 was replaced by the loop L3 of Bacillus subtilis sigmaW, 1 mM DNA (5'-D(*GP*TP*AP*AP*AP*A)-3'), 10 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O.

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
2	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
3	sodium chloride	natural abundance	50 mM
4	sodium phosphate	natural abundance	10 mM

2 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
2	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
3	sodium chloride	natural abundance	50 mM
4	sodium phosphate	natural abundance	10 mM

3 3D HNCO

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
2	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
3	sodium chloride	natural abundance	50 mM
4	sodium phosphate	natural abundance	10 mM

4 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
2	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
3	sodium chloride	natural abundance	50 mM
4	sodium phosphate	natural abundance	10 mM

5 2D 1H-13C HSQC aliphatic

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
2	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
3	sodium chloride	natural abundance	50 mM
4	sodium phosphate	natural abundance	10 mM

6 2D 1H-1H NOESY f2f

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] Engineered region2 of sigmaE from E. coli in which the loop L3 was replaced by the loop L3 of Bacillus subtilis sigmaW, 1 mM DNA (5'-D(*GP*TP*AP*AP*AP*A)-3'), 10 mM sodium phosphate, 50 mM sodium chloride, 100% D2O.

#	Name	Isotope labeling	Concentration
5	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
6	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
7	sodium chloride	natural abundance	50 mM
8	sodium phosphate	natural abundance	10 mM

7 3D C(CO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
2	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
3	sodium chloride	natural abundance	50 mM
4	sodium phosphate	natural abundance	10 mM

8 3D H(CCO)NH

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
2	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
3	sodium chloride	natural abundance	50 mM
4	sodium phosphate	natural abundance	10 mM

9 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
2	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
3	sodium chloride	natural abundance	50 mM
4	sodium phosphate	natural abundance	10 mM

10 3D 1H-13C NOESY aromatic

Instrument

Bruker AvanceIII - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
2	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
3	sodium chloride	natural abundance	50 mM
4	sodium phosphate	natural abundance	10 mM

11 3D 1H-13C NOESY aliphatic

Instrument

Bruker AvanceIII - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
2	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
3	sodium chloride	natural abundance	50 mM
4	sodium phosphate	natural abundance	10 mM

12 2D 1H-13C HSQC aromatic

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
2	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
3	sodium chloride	natural abundance	50 mM
4	sodium phosphate	natural abundance	10 mM

13 2D 1H-1H NOESY f1ff2f

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
5	DNA (5'-D(GPTPAPAPAPA)-3')	natural abundance	1 mM
6	engineered region 2 of sigmaE	[U-99% 13C; U-99% 15N]	1 mM
7	sodium chloride	natural abundance	50 mM
8	sodium phosphate	natural abundance	10 mM

nullKeywords -10 element recognition, ECF sigma factor, TRANSCRIPTION, promoter melting

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.64 seconds)

BMRB: 34172

Sample #1

Sample #2

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. ECF RNA polymerase sigma-E factor,ECF RNA polymerase sigma factor SigW,ECF RNA polymerase sigma-E factor/DNA Complex, entity 1, : 1 : 24 entities Detail

Experiments performed 13 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample