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Solution structure of the LEDGF/p75 IBD - POGZ (aa 1370-1404) complex
Authors
Veverka, V.
Assembly
PC4 and SFRS1-interacting protein,Pogo transposable element with ZNF domain
Entity
1. PC4 and SFRS1-interacting protein,Pogo transposable element with ZNF domain (polymer, Thiol state: all free), 139 monomers, 16015.92 Da Detail

SNAASWETSM DSRLQRIHAE IKNSLKIDNL DVNRCIEALD ELASLQVTMQ QAQKHTEMIT TLKKIRRFKV SQVIMEKSTM LYNKFKNMFL VGEGDSVITQ VLNKRPRSSP EETIEPESLH QLFEGESETE SFYGFEEAD


Formula weight
16015.92 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.8 %, Completeness: 92.8 %, Completeness (bb): 95.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All92.8 % (1535 of 1654)93.3 % (809 of 867)92.6 % (588 of 635)90.8 % (138 of 152)
Backbone95.4 % (790 of 828)95.3 % (266 of 279)95.6 % (395 of 413)94.9 % (129 of 136)
Sidechain91.2 % (876 of 961)92.3 % (543 of 588)90.8 % (324 of 357)56.3 % (9 of 16)
Aromatic82.0 % (82 of 100)100.0 % (50 of 50)63.3 % (31 of 49)100.0 % (1 of 1)
Methyl97.9 % (141 of 144)100.0 % (72 of 72)95.8 % (69 of 72)

1. entity 1

SNAASWETSM DSRLQRIHAE IKNSLKIDNL DVNRCIEALD ELASLQVTMQ QAQKHTEMIT TLKKIRRFKV SQVIMEKSTM LYNKFKNMFL VGEGDSVITQ VLNKRPRSSP EETIEPESLH QLFEGESETE SFYGFEEAD

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.5 mM [U-13C; U-15N] LEDGF/p75 IBD-POGZ, 50 mM TRIS, 150 mM sodium chloride, 95% H2O/5% D2O


#NameIsotope labelingTypeConcentration
1LEDGF/p75 IBD-POGZ[U-13C; U-15N]0.5 mM
2TRISnatural abundance50 mM
3sodium chloridenatural abundance150 mM

LACS Plot; CA
Referencing offset: -0.23 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: -0.23 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: 0.03 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.09 ppm, Outliers: 2 Detail
Release date
2018-07-16
Citation
Affinity switching of the LEDGF/p75 IBD interactome is governed by kinase-dependent phosphorylation
Sharma, S., Cermakova, K., De Rijck, J., Demeulemeester, J., Fabry, M., El Ashkar, S., Van Belle, S., Lepsik, M., Tesina, P., Duchoslav, V., Novak, P., Hubalek, M., Srb, P., Christ, F., Rezacova, P., Hodges, H.C., Debyser, Z., Veverka, V.
Proc. Natl. Acad. Sci. U. S. A. (2018), 115, E7053-E7062, PubMed 29997176 , DOI 10.1073/pnas.1803909115 ,
Related entities 1. PC4 and SFRS1-interacting protein,Pogo transposable element with ZNF domain, : 1 : 2 entities Detail
Experiments performed 3 experiments Detail
NMR combined restraints 4 contents Detail
Keywords TRANSCRIPTION, epigenetics, leukemia, protein-protein complex