BMRBj - BMREntryMaster

Found 1 Document (0.828 seconds)

BMRB: 34188

6ES6

Structure and dynamics conspire in the evolution of affinity between intrinsically disordered proteins

Authors

Chi, N.C.

Assembly

CID, NCBD

Entity

1. CID, NCBD, entity 1 (polymer, Thiol state: not present), 45 monomers, 4820.171 Da Detail

GSESQNDEKA LLDQLDSLLS STDEMELAEI DRALGIDKLV SQQGG

2. CID, NCBD, entity 2 (polymer, Thiol state: not present), 50 monomers, 5588.400 Da Detail

GSIPPNALQD LLRTLRSPSS PQQQQQVLNI LKSNPQLMAA FIKQRAAKYQ

Total weight

10408.57 Da

Max. entity weight

5588.4 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 74.4 %, Completeness (bb): 80.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.4 % (815 of 1096)	84.1 % (487 of 579)	58.5 % (240 of 410)	82.2 % (88 of 107)
Backbone	80.2 % (449 of 560)	94.2 % (179 of 190)	65.7 % (184 of 280)	95.6 % (86 of 90)
Sidechain	72.4 % (453 of 626)	79.2 % (308 of 389)	65.0 % (143 of 220)	11.8 % (2 of 17)
Aromatic	11.1 % (2 of 18)	22.2 % (2 of 9)	0.0 % (0 of 9)
Methyl	75.0 % (81 of 108)	90.7 % (49 of 54)	59.3 % (32 of 54)

1. entity 1

GSESQNDEKA LLDQLDSLLS STDEMELAEI DRALGIDKLV SQQGG

2. entity 2

GSIPPNALQD LLRTLRSPSS PQQQQQVLNI LKSNPQLMAA FIKQRAAKYQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 100 uM [U-99% 15N]/ U-13C CID, 100 uM [U-99% 15N]/ U-13C NCBD, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-99% 15N; U-13C]		100 uM
2	entity_2	[U-99% 15N; U-13C]		100 uM

Release date

2018-10-22

Citation

Structure and dynamics conspire in the evolution of affinity between intrinsically disordered proteins
Jemth, P., Karlsson, E., Vogeli, B., Guzovsky, B., Andersson, E., Hultqvist, G., Dogan, J., Guntert, P., Riek, R., Chi, C.N.
Sci. Adv. (2018), 4, eaau4130-eaau4130, PubMed 30397651 , DOI 10.1126/sciadv.aau4130 , Abstract

Related entities 1. CID, NCBD, entity 1, : 1 : 1 : 35 entities Detail

Related entities 2. CID, NCBD, entity 2, : 1 : 35 entities Detail

Experiments performed 8 experiments Detail

1 3D HNCACB

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 100 uM [U-99% 15N]/ U-13C CID, 100 uM [U-99% 15N]/ U-13C NCBD, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-99% 15N; U-13C]		100 uM
2	entity_2	[U-99% 15N; U-13C]		100 uM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 100 uM [U-99% 15N]/ U-13C CID, 100 uM [U-99% 15N]/ U-13C NCBD, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-99% 15N; U-13C]		100 uM
2	entity_2	[U-99% 15N; U-13C]		100 uM

3 3D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 100 uM [U-99% 15N]/ U-13C CID, 100 uM [U-99% 15N]/ U-13C NCBD, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-99% 15N; U-13C]		100 uM
2	entity_2	[U-99% 15N; U-13C]		100 uM

4 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 100 uM [U-99% 15N]/ U-13C CID, 100 uM [U-99% 15N]/ U-13C NCBD, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-99% 15N; U-13C]		100 uM
2	entity_2	[U-99% 15N; U-13C]		100 uM

5 2J HNHA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 100 uM [U-99% 15N]/ U-13C CID, 100 uM [U-99% 15N]/ U-13C NCBD, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-99% 15N; U-13C]		100 uM
2	entity_2	[U-99% 15N; U-13C]		100 uM

6 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 100 uM [U-99% 15N]/ U-13C CID, 100 uM [U-99% 15N]/ U-13C NCBD, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-99% 15N; U-13C]		100 uM
2	entity_2	[U-99% 15N; U-13C]		100 uM

7 filtered NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 100 uM [U-99% 15N]/ U-13C CID, 100 uM [U-99% 15N]/ U-13C NCBD, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-99% 15N; U-13C]		100 uM
2	entity_2	[U-99% 15N; U-13C]		100 uM

8 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 100 uM [U-99% 15N]/ U-13C CID, 100 uM [U-99% 15N]/ U-13C NCBD, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-99% 15N; U-13C]		100 uM
2	entity_2	[U-99% 15N; U-13C]		100 uM

nullKeywords CID, DNA BINDING PROTEIN, IDP, NCBD, complex

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Found 1 Document (0.828 seconds)

BMRB: 34188

Sample

Related entities 1. CID, NCBD, entity 1, : 1 : 1 : 35 entities Detail

Related entities 2. CID, NCBD, entity 2, : 1 : 35 entities Detail

Experiments performed 8 experiments Detail

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