BMRB: 34189

Structure and dynamics conspire in the evolution of affinity between intrinsically disordered proteins

Authors

Chi, N.C.

Assembly

Nuclear receptor coactivator 3 (E.C.2.3.1.48), CREB-binding protein (E.C.2.3.1.48)

Entity

1. Nuclear receptor coactivator 3 (E.C.2.3.1.48), CREB-binding protein (E.C.2.3.1.48), entity 1 (polymer, Thiol state: not present), 44 monomers, 4705.024 Da Detail

GSEGQSDERA LLDQLHTLLS NTDATGLEEI DRALGIPELV NQGQ

2. Nuclear receptor coactivator 3 (E.C.2.3.1.48), CREB-binding protein (E.C.2.3.1.48), entity 2 (polymer, Thiol state: not present), 50 monomers, 5524.352 Da Detail

GSISPSALQD LLRTLKSPSS PQQQQQVLNI LKSNPQLMAA FIKQRTAKYV

Total weight

10229.376 Da

Max. entity weight

5524.352 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 68.1 %, Completeness (bb): 77.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	68.1 % (733 of 1077)	80.7 % (456 of 565)	46.7 % (190 of 407)	82.9 % (87 of 105)
Backbone	77.4 % (429 of 554)	93.1 % (176 of 189)	61.2 % (169 of 276)	94.4 % (84 of 89)
Sidechain	62.8 % (384 of 611)	74.5 % (280 of 376)	46.1 % (101 of 219)	18.8 % (3 of 16)
Aromatic	50.0 % (11 of 22)	54.5 % (6 of 11)	45.5 % (5 of 11)
Methyl	67.2 % (78 of 116)	100.0 % (58 of 58)	34.5 % (20 of 58)

1. entity 1

GSEGQSDERA LLDQLHTLLS NTDATGLEEI DRALGIPELV NQGQ

2. entity 2

GSISPSALQD LLRTLKSPSS PQQQQQVLNI LKSNPQLMAA FIKQRTAKYV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 100 % 13C/15N CID, 100 % 13C/15N NCBD, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-15N; U-13C]		100 %
2	entity_2	[U-15N; U-13C]		100 %

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Release date

2018-10-22

Citation

Structure and dynamics conspire in the evolution of affinity between intrinsically disordered proteins
Jemth, P., Karlsson, E., Vogeli, B., Guzovsky, B., Andersson, E., Hultqvist, G., Dogan, J., Guntert, P., Riek, R., Chi, C.N.
Sci. Adv. (2018), 4, eaau4130-eaau4130, PubMed 30397651 , DOI 10.1126/sciadv.aau4130 , Abstract

Related entities 1. Nuclear receptor coactivator 3 (E.C.2.3.1.48), CREB-binding protein (E.C.2.3.1.48), entity 1, : 1 : 4 : 28 entities Detail

Related entities 2. Nuclear receptor coactivator 3 (E.C.2.3.1.48), CREB-binding protein (E.C.2.3.1.48), entity 2, : 1 : 16 : 16 entities Detail

Interaction partners 1. Nuclear receptor coactivator 3 (E.C.2.3.1.48), CREB-binding protein (E.C.2.3.1.48), entity 1, : 54 : 1 interactors Detail

Interaction partners 2. Nuclear receptor coactivator 3 (E.C.2.3.1.48), CREB-binding protein (E.C.2.3.1.48), entity 2, : 1 interactors Detail

Experiments performed 7 experiments Detail