BMRB: 34190

Proteome-wide analysis of phospho-regulated PDZ domain interactions

Authors

Chi, N.C.

Assembly

Protein scribble homolog

Entity

1. Protein scribble homolog (polymer, Thiol state: not present), 116 monomers, 12202.67 Da Detail

GAMGAPARIE EEELTLTILR QTGGLGISIA GGKGSTPYKG DDEGIFISRV SEEGPAARAG VRVGDKLLEV NGVALQGAEH HEAVEALRGA GTAVQMRVWR ERMVEPENAV TITPLR

Formula weight

12202.67 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 94.8 %, Completeness: 58.8 %, Completeness (bb): 61.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	58.8 % (735 of 1249)	80.7 % (521 of 646)	22.0 % (107 of 486)	91.5 % (107 of 117)
Backbone	61.2 % (420 of 686)	92.2 % (225 of 244)	27.8 % (92 of 331)	92.8 % (103 of 111)
Sidechain	49.2 % (326 of 662)	73.6 % (296 of 402)	10.2 % (26 of 254)	66.7 % (4 of 6)
Aromatic	26.3 % (10 of 38)	47.4 % (9 of 19)	0.0 % (0 of 18)	100.0 % (1 of 1)
Methyl	55.5 % (81 of 146)	91.8 % (67 of 73)	19.2 % (14 of 73)

1. entity 1

GAMGAPARIE EEELTLTILR QTGGLGISIA GGKGSTPYKG DDEGIFISRV SEEGPAARAG VRVGDKLLEV NGVALQGAEH HEAVEALRGA GTAVQMRVWR ERMVEPENAV TITPLR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 6.5, Details 100 % [U-100% 13C] Scribble PDZ1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Scribble PDZ1	[U-100% 13C]		100 %

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Release date

2018-08-26

Citation

Proteome-wide analysis of phospho-regulated PDZ domain interactions
Sundell, G.N., Arnold, R., Ali, M., Naksukpaiboon, P., Orts, J., Guntert, P., Chi, C.N., Ivarsson, Y.
Mol. Syst. Biol. (2018), 14, e8129-e8129, PubMed 30126976 , DOI: , Abstract

Related entities 1. Protein scribble homolog, : 1 : 15 : 206 entities Detail

Interaction partners 1. Protein scribble homolog, : 80 interactors Detail

Experiments performed 6 experiments Detail