Backbone structure of free bradykinin (BK) in DDM/CHS detergent micelle determined by MAS SSNMR
Polymer type: polypeptide(L)
Total | 13C | |
---|---|---|
All | 58.7 % (27 of 46) | 58.7 % (27 of 46) |
Backbone | 84.6 % (22 of 26) | 84.6 % (22 of 26) |
Sidechain | 42.9 % (12 of 28) | 42.9 % (12 of 28) |
Aromatic | 50.0 % (5 of 10) | 50.0 % (5 of 10) |
1. entity 1
RPPGFSPFRSolvent system 100% H2O, Pressure 1 atm, Temperature 200 K, pH 7.4, Details 0.36 ug/uL [U-13C, 15N]-F8R9 Bradykinin (BK), 50 mM not labeled HEPES, 100 mM not labeled NaCl, 2 mM not labeled EDTA, 4 % w/v not labeled DDM, 100% H2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Bradykinin (BK) | [U-13C; 15N]-F8R9 | 0.36 ug/uL | |
2 | HEPES | natural abundance | 50 mM | |
3 | NaCl | natural abundance | 100 mM | |
4 | EDTA | natural abundance | 2 mM | |
5 | DDM | natural abundance | 4 % w/v |
Solvent system 100% H2O, Pressure 1 atm, Temperature 200 K, pH 7.4, Details 0.36 ug/uL [U-13C, 15N]-P2P3G4F5S6P7F8 Bradykinin (BK), 50 mM not labeled HEPES, 100 mM not labeled NaCl, 2 mM not labeled EDTA, 4 % w/v not labeled DDM, 100% H2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | Bradykinin (BK) | [U-13C; 15N]-P2P3G4F5S6P7F8 | 0.36 ug/uL | |
7 | HEPES | natural abundance | 50 mM | |
8 | NaCl | natural abundance | 100 mM | |
9 | EDTA | natural abundance | 2 mM | |
10 | DDM | natural abundance | 4 % w/v |