BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	88.8 % (891 of 1003)	96.6 % (506 of 524)	75.9 % (290 of 382)	97.9 % (95 of 97)
Backbone	81.8 % (419 of 512)	97.7 % (170 of 174)	65.4 % (166 of 254)	98.8 % (83 of 84)
Sidechain	96.5 % (553 of 573)	96.0 % (336 of 350)	97.6 % (205 of 210)	92.3 % (12 of 13)
Aromatic	87.5 % (42 of 48)	87.5 % (21 of 24)	87.0 % (20 of 23)	100.0 % (1 of 1)
Methyl	100.0 % (96 of 96)	100.0 % (48 of 48)	100.0 % (48 of 48)

1. entity 1

SGSHMKTCVV EKIKQWIPTT EVGKILGNRA AVKKHIERQF NCVITVHTEV QSSFGATPVE IVAQNKEQCQ EARNAVMSLM QSHQDK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293.15 K, pH 5.0, Details 1 mM [U-99% 13C; U-99% 15N] MINA-1(254-334), 20 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 0.5 mM EDTA, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	MINA-1(254-334)	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM
5	EDTA	natural abundance	0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	899.6001 Hz	internal	indirect	0.2514495
¹H	water	protons	899.6001 Hz	internal	direct	1.0
¹⁵N	water	protons	899.6001 Hz	internal	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	899.6001 Hz	internal	indirect	0.2514495
¹H	water	protons	899.6001 Hz	internal	direct	1.0
¹⁵N	water	protons	899.6001 Hz	internal	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	899.6001 Hz	internal	indirect	0.2514495
¹H	water	protons	899.6001 Hz	internal	direct	1.0
¹⁵N	water	protons	899.6001 Hz	internal	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6FBL, Strand ID: A Detail

Release date

2019-01-21

Citation

MINA-1 and WAGO-4 are part of regulatory network coordinating germ cell death and RNAi in C. elegans
Sendoel, A., Subasic, D., Ducoli, L., Keller, M., Michel, E., Kohler, I., Singh, K.D., Zheng, X., Brummer, A., Imig, J., Kishore, S., Wu, Y., Kanitz, A., Kaech, A., Mittal, N., Matia-Gonzalez, A.M., Gerber, A.P., Zavolan, M., Aebersold, R., Hall, J., Allain, F.H., Hengartner, M.O.
Cell Death Differ. (2019), 26, 2157-2178, PubMed 30728462 , DOI 10.1038/s41418-019-0291-z , Abstract

Related entities 1. MINA-1, : 1 : 18 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293.15 K, pH 5.0, Details 1 mM [U-99% 13C; U-99% 15N] MINA-1(254-334), 20 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 0.5 mM EDTA, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	MINA-1(254-334)	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM
5	EDTA	natural abundance	0.5 mM

2 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MINA-1(254-334)	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM
5	EDTA	natural abundance	0.5 mM

3 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MINA-1(254-334)	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM
5	EDTA	natural abundance	0.5 mM

4 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MINA-1(254-334)	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM
5	EDTA	natural abundance	0.5 mM

5 3D HNCA

Instrument

Bruker Avance - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MINA-1(254-334)	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM
5	EDTA	natural abundance	0.5 mM

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MINA-1(254-334)	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM
5	EDTA	natural abundance	0.5 mM

7 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MINA-1(254-334)	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM
5	EDTA	natural abundance	0.5 mM

8 3D H(CCO)NH

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MINA-1(254-334)	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM
5	EDTA	natural abundance	0.5 mM

9 3D C(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MINA-1(254-334)	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM
5	EDTA	natural abundance	0.5 mM

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_34220_6fbl.nef
Input source #2: Coordindates	6fbl.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80------
SGSHMKTCVVEKIKQWIPTTEVGKILGNRAAVKKHIERQFNCVITVHTEVQSSFGATPVEIVAQNKEQCQEARNAVMSLMQSHQDK
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
SGSHMKTCVVEKIKQWIPTTEVGKILGNRAAVKKHIERQFNCVITVHTEVQSSFGATPVEIVAQNKEQCQEARNAVMSLMQSHQDK

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	86	0	0	100.0

Content subtype: combined_34220_6fbl.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	890		98.8 (chain: A, length: 86)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	OK	2744 (1)	noe	98.8 (chain: A, length: 86)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 86, Sequence coverage: 98.8%
- Total number of assignments
  - ¹H chemical shifts: 506
  - ¹³C chemical shifts: 286
  - ¹⁵N chemical shifts: 98
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
29	ARG	HH11	7.194
29	ARG	NH1	85.173
38	ARG	HH11	7.276
38	ARG	NH1	84.762
73	ARG	HH11	7.217
73	ARG	NH1	83.078

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	524	503	96.0
¹³C chemical shifts	382	286	74.9
¹⁵N chemical shifts	100	95	95.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	174	172	98.9
¹³C chemical shifts	172	85	49.4
¹⁵N chemical shifts	84	83	98.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	350	331	94.6
¹³C chemical shifts	210	201	95.7
¹⁵N chemical shifts	16	12	75.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	51	51	100.0
¹³C chemical shifts	51	51	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	24	20	83.3
¹³C chemical shifts	23	19	82.6
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 86, Sequence coverage: 98.8%
- Total number of restraints: 2668
- Weight of restraints: 1.0 (2668)
- Potential type of restraints: upper-bound-parabolic (2668)
- Range of distance target values: 2.4, 6.5 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Keywords GXXG loop, K homology (KH) domain, RNA binding protein, RNA recognition, antiparallel three-stranded beta-sheet, type-I KH domain

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Found 1 Document (0.641 seconds)

BMRB: 34220

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. MINA-1, : 1 : 18 entities Detail

Experiments performed 9 experiments Detail

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NMR combined restraints 3 contents Detail