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BMRB: 34234

6FI7

E.coli Sigma factor S (RpoS) Region 4

Authors

Liu, B., Matthews, S.J.

Assembly

RNA polymerase sigma factor RpoS

Entity

1. RNA polymerase sigma factor RpoS (polymer, Thiol state: not present), 86 monomers, 10027.27 Da Detail

GPEDTTQDDD MKQSIVKWLF ELNAKQREVL ARRFGLLGYE AATLEDVGRE IGLTRERVRQ IQVEGLRRLR EILQTQGLNI EALFRE

Formula weight

10027.27 Da

Source organism

Escherichia coli K-12

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.0 %, Completeness (bb): 99.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.0 % (1014 of 1035)	96.9 % (529 of 546)	99.0 % (390 of 394)	100.0 % (95 of 95)
Backbone	99.0 % (509 of 514)	98.9 % (176 of 178)	98.8 % (248 of 251)	100.0 % (85 of 85)
Sidechain	97.3 % (584 of 600)	95.9 % (353 of 368)	99.5 % (221 of 222)	100.0 % (10 of 10)
Aromatic	96.0 % (48 of 50)	96.0 % (24 of 25)	95.8 % (23 of 24)	100.0 % (1 of 1)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. entity 1

GPEDTTQDDD MKQSIVKWLF ELNAKQREVL ARRFGLLGYE AATLEDVGRE IGLTRERVRQ IQVEGLRRLR EILQTQGLNI EALFRE

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 1 mM [U-99% 13C; U-99% 15N] E.coli Sigma factor S region 4, 93% H2O/7% D2O

#	Name	Isotope labeling	Type	Concentration
1	E.coli Sigma factor S region 4	[U-99% 13C; U-99% 15N]		1 (±0.1) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.78 ppm	internal	indirect	0.25
¹H	water	protons	4.78 ppm	internal	indirect	1.0
¹⁵N	water	protons	4.78 ppm	internal	indirect	0.1

Referencing offset: 0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.78 ppm	internal	indirect	0.25
¹H	water	protons	4.78 ppm	internal	indirect	1.0
¹⁵N	water	protons	4.78 ppm	internal	indirect	0.1

Referencing offset: 0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.78 ppm	internal	indirect	0.25
¹H	water	protons	4.78 ppm	internal	indirect	1.0
¹⁵N	water	protons	4.78 ppm	internal	indirect	0.1

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.78 ppm	internal	indirect	0.25
¹H	water	protons	4.78 ppm	internal	indirect	1.0
¹⁵N	water	protons	4.78 ppm	internal	indirect	0.1

Referencing offset: -0.36 ppm, Outliers: 2 Detail

Release date

2018-05-28

Citation

T7 phage factor required for managing RpoS in Escherichia coli
Tabib-Salazar, A., Liu, B., Barker, D., Burchell, L., Qimron, U., Matthews, S.J., Wigneshweraraj, S.
Proc. Natl. Acad. Sci. U. S. A. (2018), 115, E5353-E5362, PubMed 29789383 , DOI 10.1073/pnas.1800429115 , Abstract

Related entities 1. RNA polymerase sigma factor RpoS, : 1 : 5 : 1 : 22 : 165 entities Detail

Interaction partners 1. RNA polymerase sigma factor RpoS, : 6 interactors Detail

More details for 6 interactors identified by 3 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC