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BMRB: 34236

6FNV

Solution structure of mule deer prion protein with polymorphism S138

Authors

Slapsak, U., Ilc, G., Plavec, J.

Assembly

Major prion protein

Entity

1. Major prion protein (polymer, Thiol state: all disulfide bound), 140 monomers, 15988.67 Da Detail

GQGGTHSQWN KPSKPKTNMK HVAGAAAAGA VVGGLGGYML GSAMSRPLIH FGNDYEDRYY RENMYRYPNQ VYYRPVDQYN NQNTFVHDCV NITVKQHTVT TTTKGENFTE TDIKMMERVV EQMCITQYQR ESQAYYQRGA

Formula weight

15988.67 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS89:SG	1:CYS124:SG

Source organism

Odocoileus hemionus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 89.3 %, Completeness (bb): 83.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.3 % (1448 of 1621)	95.6 % (808 of 845)	78.7 % (487 of 619)	97.5 % (153 of 157)
Backbone	83.7 % (695 of 830)	97.6 % (281 of 288)	68.8 % (280 of 407)	99.3 % (134 of 135)
Sidechain	95.9 % (880 of 918)	94.6 % (527 of 557)	98.5 % (334 of 339)	86.4 % (19 of 22)
Aromatic	93.7 % (148 of 158)	93.7 % (74 of 79)	93.6 % (73 of 78)	100.0 % (1 of 1)
Methyl	100.0 % (114 of 114)	100.0 % (57 of 57)	100.0 % (57 of 57)

1. Major prion protein

GQGGTHSQWN KPSKPKTNMK HVAGAAAAGA VVGGLGGYML GSAMSRPLIH FGNDYEDRYY RENMYRYPNQ VYYRPVDQYN NQNTFVHDCV NITVKQHTVT TTTKGENFTE TDIKMMERVV EQMCITQYQR ESQAYYQRGA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 5.5, Details 0.48 mM [U-99% 13C; U-99% 15N] mdPrP(S138), 20 mM sodium phosphate, 90 % H2O, 10 % [U-2H] D2O, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	mdPrP(S138)	[U-99% 13C; U-99% 15N]	0.48 mM
2	sodium phosphate	natural abundance	20 mM
3	H2O	natural abundance	90 %
4	D2O	[U-2H]	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	nitrogen	external	indirect	0.1013291

Referencing offset: 0.27 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	nitrogen	external	indirect	0.1013291

Referencing offset: 0.27 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	nitrogen	external	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6FNV, Strand ID: A Detail

Release date

2019-03-12

Citation

Solution structure of mule deer prion protein with polymorphism S138
Slapsak, U., Ilc, G., Plavec, J.

Related entities 1. Major prion protein, : 1 : 2 : 108 entities Detail

Interaction partners 1. Major prion protein, : 1 interactors Detail

More details for 1 interactors identified by 1 citations