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BMRB: 34239

6FTK

Gp36-MPER

Authors

D'Ursi, A., Grimaldi, M.

Assembly

Envelope glycoprotein gp150

Entity

1. Envelope glycoprotein gp150 (polymer, Thiol state: not present), 50 monomers, 6119.951 Da Detail

LQTKDLQQKF YEIILDIEQN NVQGKTGIQQ LQKWEDWVRW IGNIPQYLKM

Formula weight

6119.951 Da

Source organism

Feline immunodeficiency virus

Exptl. method

Refine. method

torsion angle dynamicssimulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 94.0 %, Completeness: 54.4 %, Completeness (bb): 71.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	54.4 % (361 of 663)	64.8 % (228 of 352)	34.4 % (85 of 247)	75.0 % (48 of 64)
Backbone	71.8 % (214 of 298)	92.2 % (94 of 102)	51.0 % (75 of 147)	91.8 % (45 of 49)
Sidechain	42.2 % (174 of 412)	53.6 % (134 of 250)	25.2 % (37 of 147)	20.0 % (3 of 15)
Aromatic	27.4 % (17 of 62)	35.5 % (11 of 31)	10.7 % (3 of 28)	100.0 % (3 of 3)
Methyl	41.1 % (23 of 56)	75.0 % (21 of 28)	7.1 % (2 of 28)

1. entity 1

LQTKDLQQKF YEIILDIEQN NVQGKTGIQQ LQKWEDWVRW IGNIPQYLKM

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 2 g/L [U-95% 13C] glucose, 1 g/L [U-98% 15N] ammonium sulfate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	glucose	[U-95% 13C]		2 g/L
3	ammonium sulfate	[U-98% 15N]		1 g/L

Protein Blocks Logo

Calculated from 6 models in PDB: 6FTK, Strand ID: A Detail

Release date

2018-03-26

Citation

Structural basis of antiviral activity of peptides from MPER of FIV gp36
Grimaldi, M., Stillitano, I., Amodio, G., Santoro, A., Buonocore, M., Moltedo, O., Remondelli, P., D'Ursi, A.M.
PLoS One (2018), 13, e0204042-e0204042, PubMed 30240422 , DOI 10.1371/journal.pone.0204042 , Abstract

Related entities 1. Envelope glycoprotein gp150, : 1 : 15 entities Detail

Experiments performed 10 experiments Detail

1 2D NOESY

Instrument

Bruker AvanceIII - 900 MHz TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 2 g/L [U-95% 13C] glucose, 1 g/L [U-98% 15N] ammonium sulfate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	glucose	[U-95% 13C]		2 g/L
3	ammonium sulfate	[U-98% 15N]		1 g/L

2 3D NOESY

Instrument

Bruker AvanceIII - 900 MHz TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 2 g/L [U-95% 13C] glucose, 1 g/L [U-98% 15N] ammonium sulfate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	glucose	[U-95% 13C]		2 g/L
3	ammonium sulfate	[U-98% 15N]		1 g/L

3 3D NOESY 13C-HSQC

Instrument

Bruker AvanceIII - 900 MHz TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 2 g/L [U-95% 13C] glucose, 1 g/L [U-98% 15N] ammonium sulfate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	glucose	[U-95% 13C]		2 g/L
3	ammonium sulfate	[U-98% 15N]		1 g/L

4 NOESY 15N-HSQC

Instrument

Bruker AvanceIII - 900 MHz TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 2 g/L [U-95% 13C] glucose, 1 g/L [U-98% 15N] ammonium sulfate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	glucose	[U-95% 13C]		2 g/L
3	ammonium sulfate	[U-98% 15N]		1 g/L

5 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 2 g/L [U-95% 13C] glucose, 1 g/L [U-98% 15N] ammonium sulfate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	glucose	[U-95% 13C]		2 g/L
3	ammonium sulfate	[U-98% 15N]		1 g/L

6 3D HNCACO

Instrument

Bruker AvanceIII - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 2 g/L [U-95% 13C] glucose, 1 g/L [U-98% 15N] ammonium sulfate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	glucose	[U-95% 13C]		2 g/L
3	ammonium sulfate	[U-98% 15N]		1 g/L

7 3D HNCO

Instrument

Bruker AvanceIII - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 2 g/L [U-95% 13C] glucose, 1 g/L [U-98% 15N] ammonium sulfate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	glucose	[U-95% 13C]		2 g/L
3	ammonium sulfate	[U-98% 15N]		1 g/L

8 3D HNCACB

Instrument

Bruker AvanceIII - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 2 g/L [U-95% 13C] glucose, 1 g/L [U-98% 15N] ammonium sulfate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	glucose	[U-95% 13C]		2 g/L
3	ammonium sulfate	[U-98% 15N]		1 g/L

9 3D HCCH-TOCSY

Instrument

Bruker AvanceIII - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 2 g/L [U-95% 13C] glucose, 1 g/L [U-98% 15N] ammonium sulfate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	glucose	[U-95% 13C]		2 g/L
3	ammonium sulfate	[U-98% 15N]		1 g/L

10 3D HBHA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 2 g/L [U-95% 13C] glucose, 1 g/L [U-98% 15N] ammonium sulfate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1			0.0 ~ 0.0 mM
2	glucose	[U-95% 13C]		2 g/L
3	ammonium sulfate	[U-98% 15N]		1 g/L

nullKeywords FIV, Gp36, MPER, VIRAL PROTEIN