FLN5 (full length)
MHHHHHHASK PAPSAEHSYA EGEGLVKVFD NAPAEFTIFA VDTKGVARTD GGDPFEVAIN GPDGLVVDAK VTDNNDGTYG VVYDAPVEGN YNVNVTLRGN PIKNMPIDVK CIEG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 48.2 % (593 of 1231) | 34.5 % (217 of 629) | 57.5 % (280 of 487) | 83.5 % (96 of 115) |
Backbone | 85.0 % (568 of 668) | 86.6 % (201 of 232) | 82.1 % (271 of 330) | 90.6 % (96 of 106) |
Sidechain | 16.4 % (109 of 665) | 4.0 % (16 of 397) | 35.9 % (93 of 259) | 0.0 % (0 of 9) |
Aromatic | 8.0 % (8 of 100) | 8.0 % (4 of 50) | 8.0 % (4 of 50) | |
Methyl | 16.4 % (20 of 122) | 11.5 % (7 of 61) | 21.3 % (13 of 61) |
1. entity 1
MHHHHHHASK PAPSAEHSYA EGEGLVKVFD NAPAEFTIFA VDTKGVARTD GGDPFEVAIN GPDGLVVDAK VTDNNDGTYG VVYDAPVEGN YNVNVTLRGN PIKNMPIDVK CIEGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.5, Details 200 uM [U-13C; U-15N] FLN5, 10 mM HEPES, 30 mM ammonium chloride, 12 mM magnesium chloride, 2 mM beta-mercaptoethanol, 1 mM EDTA, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FLN5 | [U-13C; U-15N] | 200 uM | |
2 | HEPES | natural abundance | 10 mM | |
3 | ammonium chloride | natural abundance | 30 mM | |
4 | magnesium chloride | natural abundance | 12 mM | |
5 | beta-mercaptoethanol | natural abundance | 2 mM | |
6 | EDTA | natural abundance | 1 mM |