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BMRB: 34256

6G5S

Solution structure of the TPR domain of the cell division coordinator, CpoB

Authors

Simorre, J.P., Maya Martinez, R.C., Bougault, C., Vollmer, W., Egan, A.

Assembly

Cell division coordinator CpoB

Entity

1. Cell division coordinator CpoB (polymer, Thiol state: not present), 146 monomers, 16126.90 Da Detail

MGSSHHHHHH SSGLVPRGSH MSGNANTDYN AAIALVQDKS RQDDAMVAFQ NFIKNYPDST YLPNANYWLG QLNYNKGKKD DAAYYFASVV KNYPKSPKAA DAMFKVGVIM QDKGDTAKAK AVYQQVISKY PGTDGAKQAQ KRLNAM

Formula weight

16126.9 Da

Source organism

Escherichia coli K-12

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.1 %, Completeness: 80.6 %, Completeness (bb): 89.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.6 % (1351 of 1677)	78.7 % (687 of 873)	83.7 % (538 of 643)	78.3 % (126 of 161)
Backbone	89.4 % (772 of 864)	87.5 % (259 of 296)	90.9 % (389 of 428)	88.6 % (124 of 140)
Sidechain	74.2 % (704 of 949)	74.2 % (428 of 577)	78.1 % (274 of 351)	9.5 % (2 of 21)
Aromatic	48.1 % (77 of 160)	62.5 % (50 of 80)	32.9 % (26 of 79)	100.0 % (1 of 1)
Methyl	92.6 % (113 of 122)	93.4 % (57 of 61)	91.8 % (56 of 61)

1. entity 1

MGSSHHHHHH SSGLVPRGSH MSGNANTDYN AAIALVQDKS RQDDAMVAFQ NFIKNYPDST YLPNANYWLG QLNYNKGKKD DAAYYFASVV KNYPKSPKAA DAMFKVGVIM QDKGDTAKAK AVYQQVISKY PGTDGAKQAQ KRLNAM

Show sample condition

Sample

Solvent system 90% H2O and 10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.5, Details 2 mM [U-13C; U-15N] CpoB, 90% H2O and 10% D2O

#	Name	Isotope labeling	Concentration
1	CpoB	[U-13C; U-15N]	2 (±0.1) mM
2	Tris/HCl	natural abundance	10 mM
3	NaCl	natural abundance	200 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.43 ppm, Outliers: 3 Detail

Release date

2018-04-03

Citation

Induced conformational changes activate the peptidoglycan synthase PBP1B
Egan, A., Maya-Martinez, R., Ayala, I., Bougault, C.M., Banzhaf, M., Breukink, E., Vollmer, W., Simorre, J.P.
Mol. Microbiol. (2018), 110, 335-356, PubMed 30044025 , DOI 10.1111/mmi.14082 , Abstract

Related entities 1. Cell division coordinator CpoB, : 1 : 2 : 11 entities Detail

Interaction partners 1. Cell division coordinator CpoB, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC