BMRBj - BMREntryMaster

Found 1 Document (6.735 seconds)

BMRB: 34259

6GBM

Solution structure of FUS-RRM bound to stem-loop RNA

Authors

Loughlin, F.E., Allain, F.H.-T.

Assembly

RNA-binding protein FUS/RNA Complex

Entity

1. RNA-binding protein FUS/RNA Complex, entity 1 (polymer, Thiol state: not present), 102 monomers, 11275.40 Da Detail

GSHMDNSDNN TIFVQGLGEN VTIESVADYF KQIGIIKTNK KTGQPMINLY TDRETGKLKG EATVSFDDPP SAKAAIDWFD GKEFSGNPIK VSFATRRADF NR

2. RNA-binding protein FUS/RNA Complex, entity 2 (polymer, Thiol state: not present), 23 monomers, 7349.307 Da Detail

GGCAGAUUAC AAUUCUAUUU GCC

Total weight

18624.707 Da

Max. entity weight

11275.4 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete1

Sequence coverage: 81.6 %, Completeness: 67.2 %, Completeness (bb): 58.3 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	67.2 % (1039 of 1545)	73.4 % (597 of 813)	55.1 % (336 of 610)	86.9 % (106 of 122)
Backbone	58.3 % (500 of 857)	57.9 % (201 of 347)	49.8 % (205 of 412)	95.9 % (94 of 98)
Sidechain	80.7 % (630 of 781)	85.0 % (396 of 466)	76.3 % (222 of 291)	50.0 % (12 of 24)
Aromatic	39.5 % (83 of 210)	48.6 % (51 of 105)	33.7 % (31 of 92)	7.7 % (1 of 13)
Methyl	97.9 % (92 of 94)	95.7 % (45 of 47)	100.0 % (47 of 47)

1. entity 1

GSHMDNSDNN TIFVQGLGEN VTIESVADYF KQIGIIKTNK KTGQPMINLY TDRETGKLKG EATVSFDDPP SAKAAIDWFD GKEFSGNPIK VSFATRRADF NR

2. entity 2

GGCAGAUUAC AAUUCUAUUU GCC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 6.5, Details 0.8 mM [U-99% 13C; U-99% 15N] FUS-RRM, 0.8 mM [U-99% 15N] RNA stem loop, 20 mM sodium phosphate, 1 mM beta-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	FUS-RRM	[U-99% 13C; U-99% 15N]	0.8 mM
2	RNA stem loop	[U-99% 15N]	0.8 mM
3	sodium phosphate	natural abundance	20 mM
4	beta-mercaptoethanol	natural abundance	1 mM

Sample #2

Solvent system 100% D2O, Pressure 1 Pa, Temperature 303 K, pH 6.5, Details 0.8 mM [U-99% 13C; U-99% 15N] FUS-RRM, 0.8 mM [U-99% 15N] stem-loop RNA, 20 mM sodium phosphate, 1 mM beta-mercaptoethanol, 100% D2O

#	Name	Isotope labeling	Concentration
5	FUS-RRM	[U-99% 13C; U-99% 15N]	0.8 mM
6	sodium phosphate	natural abundance	20 mM
7	beta-mercaptoethanol	natural abundance	1 mM
8	stem-loop RNA	[U-99% 15N]	0.8 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 15N] FUS-RRM, 0.8 mM [U-99% 13C; U-99% 15N] stem-loop RNA, 20 mM sodium phosphate, 1 mM beta-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
9	FUS-RRM	[U-99% 15N]	1 mM
10	sodium phosphate	natural abundance	20 mM
11	beta-mercaptoethanol	natural abundance	1 mM
12	stem-loop RNA	[U-99% 13C; U-99% 15N]	0.8 mM

Sample #4

Solvent system 100% D2O, Pressure 1 Pa, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] FUS-RRM, 0.8 mM [U-99% 13C; U-99% 15N] stem-loop RNA, 20 mM sodium phosphate, 1 mM beta-mercaptoethanol, 100% D2O

#	Name	Isotope labeling	Concentration
13	FUS-RRM	[U-99% 15N]	1.0 mM
14	sodium phosphate	natural abundance	20 mM
15	beta-mercaptoethanol	natural abundance	1 mM
16	stem-loop RNA	[U-99% 13C; U-99% 15N]	0.8 mM

Sample #5

Solvent system 100% D2O, Pressure 1 Pa, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] FUS-RRM, 1.0 mM [U-99% 15N] stem-loop RNA, 20 mM sodium phosphate, 1 mM beta-mercaptoethanol, 100% D2O

#	Name	Isotope labeling	Concentration
17	FUS-RRM	[U-99% 15N]	1.0 mM
18	sodium phosphate	natural abundance	20 mM
19	beta-mercaptoethanol	natural abundance	1 mM
20	stem-loop RNA	[U-99% 15N]	1.0 mM

Chem. Shift Complete2

Sequence coverage: 32.0 %, Completeness: 49.4 %, Completeness (bb): 48.8 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	49.4 % (1527 of 3090)	52.5 % (853 of 1626)	44.6 % (544 of 1220)	53.3 % (130 of 244)
Backbone	48.8 % (836 of 1714)	52.9 % (367 of 694)	43.6 % (359 of 824)	56.1 % (110 of 196)
Sidechain	51.0 % (796 of 1562)	52.1 % (486 of 932)	49.8 % (290 of 582)	41.7 % (20 of 48)
Aromatic	45.5 % (191 of 420)	50.0 % (105 of 210)	41.8 % (77 of 184)	34.6 % (9 of 26)
Methyl	52.7 % (99 of 188)	51.1 % (48 of 94)	54.3 % (51 of 94)

1. entity 1

GSHMDNSDNN TIFVQGLGEN VTIESVADYF KQIGIIKTNK KTGQPMINLY TDRETGKLKG EATVSFDDPP SAKAAIDWFD GKEFSGNPIK VSFATRRADF NR

2. entity 2

GGCAGAUUAC AAUUCUAUUU GCC

Show sample condition

Sample #1

#	Name	Isotope labeling	Concentration
1	FUS-RRM	[U-99% 13C; U-99% 15N]	0.8 mM
2	RNA stem loop	[U-99% 15N]	0.8 mM
3	sodium phosphate	natural abundance	20 mM
4	beta-mercaptoethanol	natural abundance	1 mM

Sample #2

#	Name	Isotope labeling	Concentration
5	FUS-RRM	[U-99% 13C; U-99% 15N]	0.8 mM
6	sodium phosphate	natural abundance	20 mM
7	beta-mercaptoethanol	natural abundance	1 mM
8	stem-loop RNA	[U-99% 15N]	0.8 mM

Sample #3

#	Name	Isotope labeling	Concentration
9	FUS-RRM	[U-99% 15N]	1 mM
10	sodium phosphate	natural abundance	20 mM
11	beta-mercaptoethanol	natural abundance	1 mM
12	stem-loop RNA	[U-99% 13C; U-99% 15N]	0.8 mM

Sample #4

#	Name	Isotope labeling	Concentration
13	FUS-RRM	[U-99% 15N]	1.0 mM
14	sodium phosphate	natural abundance	20 mM
15	beta-mercaptoethanol	natural abundance	1 mM
16	stem-loop RNA	[U-99% 13C; U-99% 15N]	0.8 mM

Sample #5

#	Name	Isotope labeling	Concentration
17	FUS-RRM	[U-99% 15N]	1.0 mM
18	sodium phosphate	natural abundance	20 mM
19	beta-mercaptoethanol	natural abundance	1 mM
20	stem-loop RNA	[U-99% 15N]	1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6GBM, Strand ID: B Detail

Release date

2019-03-12

Citation

The Solution Structure of FUS Bound to RNA Reveals a Bipartite Mode of RNA Recognition with Both Sequence and Shape Specificity
Loughlin, F.E., Lukavsky, P.J., Kazeeva, T., Reber, S., Hock, E.M., Colombo, M., Von Schroetter, C., Pauli, P., Clery, A., Muhlemann, O., Polymenidou, M., Ruepp, M.D., Allain, F.H.
Mol. Cell (2018), 73, 490-504, PubMed 30581145 , DOI 10.1016/j.molcel.2018.11.012 , Abstract

Related entities 1. RNA-binding protein FUS/RNA Complex, entity 1, : 1 : 9 : 256 entities Detail

Related entities 2. RNA-binding protein FUS/RNA Complex, entity 2, : 1 entities Detail

Interaction partners 1. RNA-binding protein FUS/RNA Complex, entity 1, : 117 interactors Detail

More details for 117 interactors identified by 39 citations

Experiments performed 10 experiments Detail

1 3D 1H-13C NOESY aromatic

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 6.5, Details 0.8 mM [U-99% 13C; U-99% 15N] FUS-RRM, 0.8 mM [U-99% 15N] RNA stem loop, 20 mM sodium phosphate, 1 mM beta-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	FUS-RRM	[U-99% 13C; U-99% 15N]	0.8 mM
2	RNA stem loop	[U-99% 15N]	0.8 mM
3	sodium phosphate	natural abundance	20 mM
4	beta-mercaptoethanol	natural abundance	1 mM

2 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	FUS-RRM	[U-99% 13C; U-99% 15N]	0.8 mM
2	RNA stem loop	[U-99% 15N]	0.8 mM
3	sodium phosphate	natural abundance	20 mM
4	beta-mercaptoethanol	natural abundance	1 mM

3 3D 1H-13C NOESY aliphatic

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	FUS-RRM	[U-99% 13C; U-99% 15N]	0.8 mM
2	RNA stem loop	[U-99% 15N]	0.8 mM
3	sodium phosphate	natural abundance	20 mM
4	beta-mercaptoethanol	natural abundance	1 mM

4 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 Pa, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] FUS-RRM, 0.8 mM [U-99% 13C; U-99% 15N] stem-loop RNA, 20 mM sodium phosphate, 1 mM beta-mercaptoethanol, 100% D2O

#	Name	Isotope labeling	Concentration
13	FUS-RRM	[U-99% 15N]	1.0 mM
14	sodium phosphate	natural abundance	20 mM
15	beta-mercaptoethanol	natural abundance	1 mM
16	stem-loop RNA	[U-99% 13C; U-99% 15N]	0.8 mM

5 3D 1H-13C NOESY aromatic

Instrument

Bruker AvanceIII - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
13	FUS-RRM	[U-99% 15N]	1.0 mM
14	sodium phosphate	natural abundance	20 mM
15	beta-mercaptoethanol	natural abundance	1 mM
16	stem-loop RNA	[U-99% 13C; U-99% 15N]	0.8 mM

6 2D 1H-1H NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 Pa, Temperature 303 K, pH 6.5, Details 1.0 mM [U-99% 15N] FUS-RRM, 1.0 mM [U-99% 15N] stem-loop RNA, 20 mM sodium phosphate, 1 mM beta-mercaptoethanol, 100% D2O

#	Name	Isotope labeling	Concentration
17	FUS-RRM	[U-99% 15N]	1.0 mM
18	sodium phosphate	natural abundance	20 mM
19	beta-mercaptoethanol	natural abundance	1 mM
20	stem-loop RNA	[U-99% 15N]	1.0 mM

7 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 303 K, pH 6.5, Details 1 mM [U-99% 15N] FUS-RRM, 0.8 mM [U-99% 13C; U-99% 15N] stem-loop RNA, 20 mM sodium phosphate, 1 mM beta-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
9	FUS-RRM	[U-99% 15N]	1 mM
10	sodium phosphate	natural abundance	20 mM
11	beta-mercaptoethanol	natural abundance	1 mM
12	stem-loop RNA	[U-99% 13C; U-99% 15N]	0.8 mM

8 2D 1H-15N HSQC

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	FUS-RRM	[U-99% 13C; U-99% 15N]	0.8 mM
2	RNA stem loop	[U-99% 15N]	0.8 mM
3	sodium phosphate	natural abundance	20 mM
4	beta-mercaptoethanol	natural abundance	1 mM

9 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 Pa, Temperature 303 K, pH 6.5, Details 0.8 mM [U-99% 13C; U-99% 15N] FUS-RRM, 0.8 mM [U-99% 15N] stem-loop RNA, 20 mM sodium phosphate, 1 mM beta-mercaptoethanol, 100% D2O

#	Name	Isotope labeling	Concentration
5	FUS-RRM	[U-99% 13C; U-99% 15N]	0.8 mM
6	sodium phosphate	natural abundance	20 mM
7	beta-mercaptoethanol	natural abundance	1 mM
8	stem-loop RNA	[U-99% 15N]	0.8 mM

10 2D 1H-13C HSQC

Instrument

Bruker Avance - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
13	FUS-RRM	[U-99% 15N]	1.0 mM
14	sodium phosphate	natural abundance	20 mM
15	beta-mercaptoethanol	natural abundance	1 mM
16	stem-loop RNA	[U-99% 13C; U-99% 15N]	0.8 mM

nullKeywords RNA, RNA BINDING PROTEIN, RNA recognition motif, RRM

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Found 1 Document (6.735 seconds)

BMRB: 34259

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. RNA-binding protein FUS/RNA Complex, entity 1, : 1 : 9 : 256 entities Detail

Related entities 2. RNA-binding protein FUS/RNA Complex, entity 2, : 1 entities Detail

Interaction partners 1. RNA-binding protein FUS/RNA Complex, entity 1, : 117 interactors Detail

Experiments performed 10 experiments Detail

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