BMRB: 34263

Calmodulin mutant - F141L apo-form Unstructured C-domain

Authors

Holt, C., Overgaard, M.T., Wimmer, R.

Assembly

Calmodulin-1

Entity

1. Calmodulin-1 (polymer, Thiol state: not present), 148 monomers, 16672.20 Da Detail

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE LVQMMTAK

Formula weight

16672.2 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 72.3 %, Completeness: 63.0 %, Completeness (bb): 69.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	63.0 % (1049 of 1666)	61.0 % (528 of 865)	65.6 % (422 of 643)	62.7 % (99 of 158)
Backbone	69.1 % (611 of 884)	68.5 % (209 of 305)	70.0 % (303 of 433)	67.8 % (99 of 146)
Sidechain	58.1 % (534 of 919)	57.0 % (319 of 560)	62.0 % (215 of 347)	0.0 % (0 of 12)
Aromatic	45.6 % (41 of 90)	48.9 % (22 of 45)	42.2 % (19 of 45)
Methyl	68.5 % (100 of 146)	68.5 % (50 of 73)	68.5 % (50 of 73)

1. entity 1

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE LVQMMTAK

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Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.1 K, pH 6.55, Details 580 uM [U-99% 13C; U-99% 15N] Calmodulin, 2 mM HEPES, 10 mM potassium chloride, 10 mM EDTA, 2 mM sodium azide, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Calmodulin	[U-99% 13C; U-99% 15N]		580 uM
2	HEPES	natural abundance		2 mM
3	potassium chloride	natural abundance		10 mM
4	EDTA	natural abundance		10 mM
5	sodium azide	natural abundance		2 mM

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LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	TSP	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	TSP	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	TSP	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.34 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	TSP	methyl protons	0.0 ppm	internal	indirect	0.1013291

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Release date

2018-10-22

Citation

Arrhythmia mutations in calmodulin cause conformational changes that affect interactions with the cardiac voltage-gated calcium channel
Wang, K., Holt, C., Lu, J., Brohus, M., Larsen, K.T., Overgaard, M.T., Wimmer, R., Petegem, F.V.
Proc. Natl. Acad. Sci. U. S. A. (2018), 115, E10556-E10565, PubMed 30348784 , DOI 10.1073/pnas.1808733115 , Abstract

Related entities 1. Calmodulin-1, : 1 : 3 : 111 : 181 entities Detail

Interaction partners 1. Calmodulin-1, : 1 interactors Detail

Experiments performed 18 experiments Detail