BMRB: 34267

Antinociceptive evaluation of cyriotoxin-1a, the first toxin purified from Cyriopagopus schioedtei spider venom

Authors

Kurz, M.

Assembly

cyriotoxin-1a

Entity

1. cyriotoxin-1a (polymer, Thiol state: all disulfide bound), 34 monomers, 3586.258 Da Detail

ECKGFGKSCV PGKNECCSGL TCSNKHKWCK VLLX

Formula weight

3586.258 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS17:SG
2	disulfide	sing	1:CYS9:SG	1:CYS22:SG
3	disulfide	sing	1:CYS16:SG	1:CYS29:SG

Source organism

Cyriopagopus schioedtei

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 99.5 %, Completeness (bb): 98.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	99.5 % (198 of 199)	99.5 % (198 of 199)
Backbone	98.6 % (68 of 69)	98.6 % (68 of 69)
Sidechain	100.0 % (130 of 130)	100.0 % (130 of 130)
Aromatic	100.0 % (13 of 13)	100.0 % (13 of 13)
Methyl	100.0 % (11 of 11)	100.0 % (11 of 11)

1. cyriotoxin-1a

ECKGFGKSCV PGKNECCSGL TCSNKHKWCK VLLX

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 305 K, pH 5.0, Details 6 mg/mL unlabeled cyriotoxin-1a, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	cyriotoxin-1a	unlabeled		6 (±0.2) mg/mL

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Protein Blocks Logo

Calculated from 20 models in PDB: 6GFT, Strand ID: A Detail

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Release date

2019-03-12

Citation

From identification to functional characterization of cyriotoxin-1a, an antinociceptive toxin from Cyriopagopus schioedtei spider
Goncalves, T.C., Benoit, E., Kurz, M., Lucarain, L., Fouconnier, S., Combemale, S., Jaquillard, L., Schombert, B., Chambard, J.M., Boukaiba, R., Hessler, G., Bohme, A., Bialy, L., Hourcade, S., Beroud, R., De Waard, M., Servent, D., Partiseti, M.
Br. J. Pharmacol. (2019), 176, 1298-1314, PubMed 30784059 , DOI 10.1111/bph.14628 , Abstract

Related entities 1. cyriotoxin-1a, : 1 : 130 entities Detail

Experiments performed 5 experiments Detail