BMRBj - BMREntryMaster

Found 1 Document (0.533 seconds)

BMRB: 34269

6GH0

Two-quartet kit* G-quadruplex is formed via double-stranded pre-folded structure

Authors

Kotar, A., Rigo, R., Sissi, C., Plavec, J.

Assembly

DNA (5'-D(*GP*GP*CP*GP*AP*GP*GP*AP*GP*GP*GP*GP*CP*GP*TP*GP*GP*CP*CP*GP*GP*C)-3')

Entity

1. DNA (5'-D(*GP*GP*CP*GP*AP*GP*GP*AP*GP*GP*GP*GP*CP*GP*TP*GP*GP*CP*CP*GP*GP*C)-3') (polymer, Thiol state: not present), 22 monomers, 7003.410 Da Detail

GGCGAGGAGG GGCGTGGCCG GC

Formula weight

7003.41 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.4 %, Completeness (bb): 96.1 % Detail

Polymer type: polydeoxyribonucleotide

	Total	¹H
All	90.4 % (189 of 209)	90.4 % (189 of 209)
Suger, PO4	96.1 % (148 of 154)	96.1 % (148 of 154)
Nucleobase	74.5 % (41 of 55)	74.5 % (41 of 55)
Aromatic	86.4 % (38 of 44)	86.4 % (38 of 44)
Methyl	100.0 % (1 of 1)	100.0 % (1 of 1)

1. entity 1

GGCGAGGAGG GGCGTGGCCG GC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4, Details 0.5 mM 10% 13C, 10% 15N DNA (5'-D(*GP*GP*CP*GP*AP*GP*GP*AP*GP*GP*GP*GP*CP*GP*TP*GP*GP*CP*CP*GP*GP*C)-3'), 100 mM potassium chloride, 20 mM potassium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	[10% 13C; 10% 15N]	0.5 mM
2	potassium chloride	natural abundance	100 mM
3	potassium phosphate	natural abundance	20 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4, Details 0.4 mM DNA (5'-D(*GP*GP*CP*GP*AP*GP*GP*AP*GP*GP*GP*GP*CP*GP*TP*GP*GP*CP*CP*GP*GP*C)-3'), 100 mM potassium chloride, 20 mM potassium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	natural abundance	0.4 mM
5	potassium chloride	natural abundance	100 mM
6	potassium phosphate	natural abundance	20 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4, Details 0.4 mM DNA (5'-D(*GP*GP*CP*GP*AP*GP*GP*AP*GP*GP*GP*GP*CP*GP*TP*GP*GP*CP*CP*GP*GP*C)-3'), 100 mM potassium chloride, 20 mM potassium phosphate, 100% D2O

#	Name	Isotope labeling	Concentration
7	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	natural abundance	0.4 mM
8	potassium chloride	natural abundance	100 mM
9	potassium phosphate	natural abundance	20 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4, Details 0.5 mM DNA (5'-D(*GP*GP*CP*GP*AP*GP*GP*AP*GP*GP*GP*GP*CP*GP*TP*GP*GP*CP*CP*GP*GP*C)-3'), 100 mM potassium chloride, 20 mM potassium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
10	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	natural abundance	0.5 mM
11	potassium chloride	natural abundance	100 mM
12	potassium phosphate	natural abundance	20 mM

Release date

2019-01-01

Citation

Two-quartet kit* G-quadruplex is formed via double-stranded pre-folded structure
Kotar, A., Rigo, R., Sissi, C., Plavec, J.
Nucleic Acids Res. (2018), 47, 2641-2653, PubMed 30590801 , DOI 10.1093/nar/gky1269 , Abstract

Experiments performed 12 experiments Detail

1 2D 1H-1H NOESY

Instrument

Varian Uniform NMR System - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4, Details 0.5 mM DNA (5'-D(*GP*GP*CP*GP*AP*GP*GP*AP*GP*GP*GP*GP*CP*GP*TP*GP*GP*CP*CP*GP*GP*C)-3'), 100 mM potassium chloride, 20 mM potassium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
10	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	natural abundance	0.5 mM
11	potassium chloride	natural abundance	100 mM
12	potassium phosphate	natural abundance	20 mM

2 2D 1H-1H NOESY

Instrument

Varian Uniform NMR System - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
10	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	natural abundance	0.5 mM
11	potassium chloride	natural abundance	100 mM
12	potassium phosphate	natural abundance	20 mM

3 1D 1H-15N HSQC

Instrument

Varian Uniform NMR System - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4, Details 0.5 mM 10% 13C, 10% 15N DNA (5'-D(*GP*GP*CP*GP*AP*GP*GP*AP*GP*GP*GP*GP*CP*GP*TP*GP*GP*CP*CP*GP*GP*C)-3'), 100 mM potassium chloride, 20 mM potassium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	[10% 13C; 10% 15N]	0.5 mM
2	potassium chloride	natural abundance	100 mM
3	potassium phosphate	natural abundance	20 mM

4 2D 1H-15N HSQC

Instrument

Varian Uniform NMR System - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	[10% 13C; 10% 15N]	0.5 mM
2	potassium chloride	natural abundance	100 mM
3	potassium phosphate	natural abundance	20 mM

5 2D 1H-13C HSQC

Instrument

Varian Uniform NMR System - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	[10% 13C; 10% 15N]	0.5 mM
2	potassium chloride	natural abundance	100 mM
3	potassium phosphate	natural abundance	20 mM

6 2D 1H-1H TOCSY

Instrument

Varian Uniform NMR System - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.8, Details 0.4 mM DNA (5'-D(*GP*GP*CP*GP*AP*GP*GP*AP*GP*GP*GP*GP*CP*GP*TP*GP*GP*CP*CP*GP*GP*C)-3'), 100 mM potassium chloride, 20 mM potassium phosphate, 100% D2O

#	Name	Isotope labeling	Concentration
7	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	natural abundance	0.4 mM
8	potassium chloride	natural abundance	100 mM
9	potassium phosphate	natural abundance	20 mM

7 2D 1H-13C HSQC aromatic

Instrument

Varian Uniform NMR System - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4, Details 0.4 mM DNA (5'-D(*GP*GP*CP*GP*AP*GP*GP*AP*GP*GP*GP*GP*CP*GP*TP*GP*GP*CP*CP*GP*GP*C)-3'), 100 mM potassium chloride, 20 mM potassium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
4	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	natural abundance	0.4 mM
5	potassium chloride	natural abundance	100 mM
6	potassium phosphate	natural abundance	20 mM

8 2D 1H-15N HSQC NH2 only

Instrument

Varian Uniform NMR System - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	[10% 13C; 10% 15N]	0.5 mM
2	potassium chloride	natural abundance	100 mM
3	potassium phosphate	natural abundance	20 mM

9 2D DQF-COSY

Instrument

Varian Uniform NMR System - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	natural abundance	0.4 mM
8	potassium chloride	natural abundance	100 mM
9	potassium phosphate	natural abundance	20 mM

10 2D 1H-1H NOESY

Instrument

Varian Uniform NMR System - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
4	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	natural abundance	0.4 mM
5	potassium chloride	natural abundance	100 mM
6	potassium phosphate	natural abundance	20 mM

11 2D 1H-1H NOESY

Instrument

Varian Uniform NMR System - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
4	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	natural abundance	0.4 mM
5	potassium chloride	natural abundance	100 mM
6	potassium phosphate	natural abundance	20 mM

12 2D 1H-1H NOESY

Instrument

Varian Uniform NMR System - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
7	DNA (5'-D(GPGPCPGPAPGPGPAPGPGPGPGPCPGPTPGPGPCPCPGPGPC)-3')	natural abundance	0.4 mM
8	potassium chloride	natural abundance	100 mM
9	potassium phosphate	natural abundance	20 mM

nullKeywords DNA, G-quadruplex, c-KIT promoter, two G-quartets

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Found 1 Document (0.533 seconds)

BMRB: 34269

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Experiments performed 12 experiments Detail

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