BMRBj - BMREntryMaster

Found 1 Document (0.488 seconds)

BMRB: 34271

6GIG

Structural insights into AapA1 toxin

Authors

Salgado, G.F., Korkut, D.N.

Assembly

AapA1

Entity

1. AapA1 (polymer, Thiol state: all free), 30 monomers, 3463.251 Da Detail

MATKHGKNSW KTLYLKISFL GCKVVVLLKR

Formula weight

3463.251 Da

Source organism

Helicobacter pylori

Exptl. method

solution NMR

Refine. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.0 %, Completeness (bb): 97.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	94.0 % (219 of 233)	93.5 % (188 of 201)	96.9 % (31 of 32)
Backbone	97.8 % (90 of 92)	98.4 % (61 of 62)	96.7 % (29 of 30)
Sidechain	91.5 % (129 of 141)	91.4 % (127 of 139)	100.0 % (2 of 2)
Aromatic	83.3 % (15 of 18)	82.4 % (14 of 17)	100.0 % (1 of 1)
Methyl	81.0 % (17 of 21)	81.0 % (17 of 21)

1. entity 1

MATKHGKNSW KTLYLKISFL GCKVVVLLKR

Show sample condition

Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 303 (±1) K, pH 4.5 (±0.1), Details 2 mM AapA1, 0.2 mM AapA1, trifluoroethanol/water

#	Name	Isotope labeling	Type	Concentration
1	AapA1_1	natural abundance		2 (±0.1) mM
2	AapA1_2	natural abundance		0.2 (±0.05) mM

Protein Blocks Logo

Calculated from 10 models in PDB: 6GIG, Strand ID: A Detail

Release date

2019-09-03

Citation

Structural insights into the AapA1 toxin of Helicobacter pylori
Korkut, D.N., Alves, I.D., Vogel, A., Chabas, S., Sharma, C.M., Martinez, D., Loquet, A., Salgado, G.F., Darfeuille, F.
Biochim. Biophys. Acta. Gen. Subj. (2019), 129423-129423, PubMed 31476357 , DOI 10.1016/j.bbagen.2019.129423 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 34270 released on 2018-05-15
    Title AapA1 V26A toxin from helicobacter pylori 26695
  BMRB: 27471 released on 2018-05-02
    Title peptide AapA1 toxin of Helicobacter pylori

Related entities 1. AapA1, : 1 : 1 : 2 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34271_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34271_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	253		100.0 (chain: 1, length: 30)
1	Chemical shift references	chem_shift_reference_1	OK	2		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 30, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 222
  - ¹⁵N chemical shifts: 31
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
5	HIS	HD1	7.349

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	201	187	93.0
¹⁵N chemical shifts	33	31	93.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	62	61	98.4
¹⁵N chemical shifts	30	29	96.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	139	126	90.6
¹⁵N chemical shifts	3	2	66.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	22	17	77.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	17	14	82.4
¹⁵N chemical shifts	1	1	100.0

Keywords ANTITOXIN, Toxin peptide from Helicobacter pylori

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.488 seconds)

BMRB: 34271

Sample

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. AapA1, : 1 : 1 : 2 entities Detail

Experiments performed 5 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail