BMRB: 34272

NMR structure of temporin B KKG6A in SDS micelles

Authors

Manzo, G., Mason, J.A.

Assembly

temporinB KKG6A

Entity

1. temporinB KKG6A (polymer, Thiol state: not present), 15 monomers, 1663.138 Da Detail

KKLLPIVANL LKSLL

Formula weight

1663.138 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 81.7 %, Completeness (bb): 89.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	81.7 % (85 of 104)	81.7 % (85 of 104)
Backbone	89.7 % (26 of 29)	89.7 % (26 of 29)
Sidechain	78.7 % (59 of 75)	78.7 % (59 of 75)
Methyl	35.3 % (6 of 17)	35.3 % (6 of 17)

1. temporinB KKG6A

KKLLPIVANL LKSLL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.00, Details 100 mM U-98% 2H deuterated sodium dodecyl sulphate, 2 mM temporinB_KKG6A, 0.05 % w/w 2H 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	deuterated sodium dodecyl sulphate	U-98% 2H		100 mM
2	temporinB_KKG6A	natural abundance		2 mM
3	3-(trimethylsilyl)propionic-2,2,3,3-d4 acid	2H		0.05 % w/w

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Release date

2018-06-05

Citation

Minor sequence modifications in temporin B cause drastic changes in antibacterial potency and selectivity by fundamentally altering membrane activity
Manzo, G., Ferguson, P.M., Gustilo, V.B., Hind, C.K., Clifford, M., Bui, T.T., Drake, A.F., Atkinson, R.A., Sutton, J.M., Batoni, G., Lorenz, C.D., Phoenix, D.A., Mason, A.J.
Sci. Rep. (2019), 9, 1385-1385, PubMed 30718667 , DOI 10.1038/s41598-018-37630-3 , Abstract

Related entities 1. temporinB KKG6A, : 1 : 2 entities Detail

Experiments performed 2 experiments Detail