BMRB: 34273

NMR structure of temporin B L1FK in SDS micelles

Authors

Manzo, G., Mason, J.A.

Assembly

temporinB L1FK

Entity

1. temporinB L1FK (polymer, Thiol state: not present), 13 monomers, 1440.854 Da Detail

FLPIVGLLKS LLK

Formula weight

1440.854 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 77.8 %, Completeness (bb): 84.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	77.8 % (70 of 90)	77.8 % (70 of 90)
Backbone	84.6 % (22 of 26)	84.6 % (22 of 26)
Sidechain	75.0 % (48 of 64)	75.0 % (48 of 64)
Aromatic	0.0 % (0 of 5)	0.0 % (0 of 5)
Methyl	50.0 % (7 of 14)	50.0 % (7 of 14)

1. temporinB L1FK

FLPIVGLLKS LLK

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 100 mM U-98% 2H deuterated sodium dodecyl sulphate (d-25), 2 mM temporinB_L1FK, 0.05 % w/w 2H 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	deuterated sodium dodecyl sulphate (d-25)	U-98% 2H		100 mM
2	temporinB_L1FK	natural abundance		2 mM
3	3-(trimethylsilyl)propionic-2,2,3,3-d4 acid	2H		0.05 % w/w

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Release date

2018-06-05

Citation

Minor sequence modifications in temporin B cause drastic changes in antibacterial potency and selectivity by fundamentally altering membrane activity
Manzo, G., Ferguson, P.M., Gustilo, V.B., Hind, C.K., Clifford, M., Bui, T.T., Drake, A.F., Atkinson, R.A., Sutton, J.M., Batoni, G., Lorenz, C.D., Phoenix, D.A., Mason, A.J.
Sci. Rep. (2019), 9, 1385-1385, PubMed 30718667 , DOI 10.1038/s41598-018-37630-3 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. temporinB L1FK, : 1 entities Detail

Experiments performed 2 experiments Detail