BMRB: 34274

NMR structure of temporin B in SDS micelles

Authors

Manzo, G., Mason, J.A.

Assembly

Temporin-B

Entity

1. Temporin-B (polymer, Thiol state: not present), 13 monomers, 1392.769 Da Detail

LLPIVGNLLK SLL

Formula weight

1392.769 Da

Source organism

Rana temporaria

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 92.3 %, Completeness: 83.3 %, Completeness (bb): 92.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	83.3 % (70 of 84)	83.3 % (70 of 84)
Backbone	92.3 % (24 of 26)	92.3 % (24 of 26)
Sidechain	79.3 % (46 of 58)	79.3 % (46 of 58)
Methyl	56.3 % (9 of 16)	56.3 % (9 of 16)

1. Temporin-B

LLPIVGNLLK SLL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.00, Details 100 mM U-98% 2H deuterated sodium dodecyl sulphate, 2 mM temporin B, 0.05 % w/w 2H 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	deuterated sodium dodecyl sulphate	U-98% 2H		100 mM
2	temporin B	natural abundance		2 mM
3	3-(trimethylsilyl)propionic-2,2,3,3-d4 acid	2H		0.05 % w/w

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Release date

2018-06-05

Citation

Minor sequence modifications in temporin B cause drastic changes in antibacterial potency and selectivity by fundamentally altering membrane activity
Manzo, G., Ferguson, P.M., Gustilo, V.B., Hind, C.K., Clifford, M., Bui, T.T., Drake, A.F., Atkinson, R.A., Sutton, J.M., Batoni, G., Lorenz, C.D., Phoenix, D.A., Mason, A.J.
Sci. Rep. (2019), 9, 1385-1385, PubMed 30718667 , DOI 10.1038/s41598-018-37630-3 , Abstract

Related entities 1. Temporin-B, : 1 : 1 : 2 entities Detail

Experiments performed 2 experiments Detail