BMRB: 34284

NMR structure of aurein 2.5 in SDS micelles

Authors

Manzo, G., Mason, J.A.

Assembly

Temporin-B

Entity

1. Temporin-B (polymer, Thiol state: not present), 16 monomers, 1649.970 Da Detail

GLFDIVKKVV GAFGSL

Formula weight

1649.97 Da

Source organism

Ranoidea aurea

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.4 %, Completeness (bb): 97.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	90.4 % (85 of 94)	90.4 % (85 of 94)
Backbone	97.1 % (34 of 35)	97.1 % (34 of 35)
Sidechain	86.4 % (51 of 59)	86.4 % (51 of 59)
Aromatic	20.0 % (2 of 10)	20.0 % (2 of 10)
Methyl	100.0 % (13 of 13)	100.0 % (13 of 13)

1. entity 1

GLFDIVKKVV GAFGSL

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 2 mM Aurein 2.5, 100 mM [U-98% 2H] deuterated sodium dodecyl sulphate, 0.05 % w/w 2H 3-(Trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Aurein 2.5	natural abundance		2 mM
2	deuterated sodium dodecyl sulphate	[U-98% 2H]		100 mM
3	3-(Trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt	[U-2H]		0.05 % w/w

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Release date

2018-07-08

Citation

Temporin L and aurein 2.5 have identical conformations but subtly distinct membrane and antibacterial activities
Manzo, G., Ferguson, P.M., Hind, C.K., Clifford, M., Gustilo, V.B., Ali, H., Bansal, S.S., Bui, T.T., Drake, A.F., Atkinson, R.A., Sutton, J.M., Lorenz, C.D., Phoenix, D.A., Mason, A.J.
Sci. Rep. (2019), 9, 10934-10934, PubMed 31358802 , DOI 10.1038/s41598-019-47327-w , Abstract

Related entities 1. Temporin-B, : 1 : 1 : 4 : 4 entities Detail

Experiments performed 2 experiments Detail