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BMRB: 34285

6GSE

Solution structure of the capsid domain from the activity-regulated cytoskeleton-associated protein, Arc

Authors

Nielsen, L.D., Erlendsson, S., Teilum, K.

Assembly

Activity-regulated cytoskeleton-associated protein

Entity

1. Activity-regulated cytoskeleton-associated protein (polymer, Thiol state: not present), 159 monomers, 18973.11 Da Detail

SPGLDTQIFE DPREFLSHLE EYLRQVGGSE EYWLSQIQNH MNGPAKKWWE FKQGSVKNWV EFKKEFLQYS EGTLSREAIQ RELDLPQKQG EPLDQFLWRK RDLYQTLYVD AEEEEIIQYV VGTLQPKFKR FLRHPLPKTL EQLIQRGMEV QDGLEQAAE

Formula weight

18973.11 Da

Source organism

Rattus norvegicus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 81.9 %, Completeness (bb): 95.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.9 % (1639 of 2001)	79.1 % (838 of 1060)	84.4 % (646 of 765)	88.1 % (155 of 176)
Backbone	95.8 % (899 of 938)	93.8 % (300 of 320)	96.6 % (451 of 467)	98.0 % (148 of 151)
Sidechain	73.0 % (885 of 1212)	72.7 % (538 of 740)	76.1 % (340 of 447)	28.0 % (7 of 25)
Aromatic	61.5 % (123 of 200)	70.0 % (70 of 100)	50.5 % (48 of 95)	100.0 % (5 of 5)
Methyl	89.2 % (132 of 148)	90.5 % (67 of 74)	87.8 % (65 of 74)

1. entity 1

SPGLDTQIFE DPREFLSHLE EYLRQVGGSE EYWLSQIQNH MNGPAKKWWE FKQGSVKNWV EFKKEFLQYS EGTLSREAIQ RELDLPQKQG EPLDQFLWRK RDLYQTLYVD AEEEEIIQYV VGTLQPKFKR FLRHPLPKTL EQLIQRGMEV QDGLEQAAE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 0.5 mM [U-15N] Arc, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Arc	[U-15N]		0.5 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 1 mM [U-13C; U-15N] Arc, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	Arc	[U-13C; U-15N]		1 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 7.4, Details 0.5 mM [U-15N] Arc, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Arc	[U-15N]		0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: -0.41 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: -0.41 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: -0.37 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6GSE, Strand ID: A Detail

Release date

2019-05-12

Citation

The Capsid Domain of Arc Changes Its Oligomerization Propensity through Direct Interaction with the NMDA Receptor
Nielsen, L.D., Pedersen, C.P., Erlendsson, S., Teilum, K.
Structure (2019), 27, 1071-1081, PubMed 31080121 , DOI 10.1016/j.str.2019.04.001 , Abstract

Related entities 1. Activity-regulated cytoskeleton-associated protein, : 1 : 3 : 19 entities Detail

Interaction partners 1. Activity-regulated cytoskeleton-associated protein, : 6 interactors Detail

More details for 6 interactors identified by 2 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC