BMRB: 34297

Adenine-driven structural switch from two- to three-quartet DNA G-quadruplex

Authors

Lenarcic Zivkovic, M., Rozman, J., Plavec, J.

Assembly

DNA (5'-D(*GP*GP*GP*TP*AP*GP*GP*GP*AP*GP*CP*GP*GP*GP*AP*GP*AP*GP*GP*G)-3')

Entity

1. DNA (5'-D(*GP*GP*GP*TP*AP*GP*GP*GP*AP*GP*CP*GP*GP*GP*AP*GP*AP*GP*GP*G)-3') (polymer, Thiol state: not present), 20 monomers, 6473.097 Da Detail

GGGTAGGGAG CGGGAGAGGG

Formula weight

6473.097 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 79.2 %, Completeness (bb): 77.1 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	79.2 % (145 of 183)	79.2 % (145 of 183)
Suger, PO4	77.1 % (108 of 140)	77.1 % (108 of 140)
Nucleobase	86.0 % (37 of 43)	86.0 % (37 of 43)
Aromatic	90.0 % (36 of 40)	90.0 % (36 of 40)
Methyl	100.0 % (1 of 1)	100.0 % (1 of 1)

1. entity 1

GGGTAGGGAG CGGGAGAGGG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 7.0, Details 0.6 mM DNA (5'-D(*GP*GP*GP*TP*AP*GP*GP*GP*AP*GP*CP*GP*GP*GP*AP*GP*AP*GP*GP*G)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*GP*GP*GP*TP*AP*GP*GP*GP*AP*GP*CP*GP*GP*GP*AP*GP*AP*GP*GP*G)-3')	natural abundance		0.6 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 278 K, pH 7.0, Details 0.8 mM DNA (5'-D(*GP*GP*GP*TP*AP*GP*GP*GP*AP*GP*CP*GP*GP*GP*AP*GP*AP*GP*GP*G)-3'), 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	DNA (5'-D(*GP*GP*GP*TP*AP*GP*GP*GP*AP*GP*CP*GP*GP*GP*AP*GP*AP*GP*GP*G)-3')	natural abundance		0.8 mM

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Release date

2018-09-19

Citation

Adenine-Driven Structural Switch from a Two- to Three-Quartet DNA G-Quadruplex
Lenarcic Zivkovic, M., Rozman, J., Plavec, J.
Angew. Chem Int Ed Engl. (2018), 57, 15395-15399, PubMed 30222243 , DOI 10.1002/anie.201809328 , Abstract

Experiments performed 5 experiments Detail