BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	96.7 % (1576 of 1630)	97.9 % (832 of 850)	95.0 % (608 of 640)	97.1 % (136 of 140)
Backbone	97.8 % (782 of 800)	96.7 % (264 of 273)	98.7 % (392 of 397)	96.9 % (126 of 130)
Sidechain	96.1 % (920 of 957)	98.4 % (568 of 577)	92.4 % (342 of 370)	100.0 % (10 of 10)
Aromatic	85.1 % (126 of 148)	100.0 % (74 of 74)	69.0 % (49 of 71)	100.0 % (3 of 3)
Methyl	93.2 % (138 of 148)	91.9 % (68 of 74)	94.6 % (70 of 74)

1. entity 1

MGWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL VPSDITVAQF MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD EDGFLYVAYS GENTFG

2. entity 2

GAMEIIHEDN EWGIELVSE

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 7.0 (±0.1), Details 0.6 mM [U-99% 13C; U-99% 15N] GABARAPL2, 1.0 mM No Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif, 50 mM No sodium phosphate, 100 mM No sodium chloride, 4.6 mM No sodium azide, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	GABARAPL2	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	natural abundance	1.0 (±0.05) mM
3	sodium phosphate	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	4.6 (±0.1) mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 7.0 (±0.1), Details 1.0 mM No GABARAPL2, 0.6 mM [U-99% 13C; U-99% 15N] Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif, 50 mM No sodium phosphate, 100 mM No sodium chloride, 4.6 mM No sodium azide, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
6	GABARAPL2	natural abundance	1.0 (±0.05) mM
7	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
8	sodium phosphate	natural abundance	50 (±1.0) mM
9	sodium chloride	natural abundance	100 (±1.0) mM
10	sodium azide	natural abundance	4.6 (±0.1) mM

Protein Blocks Logo

Calculated from 20 models in PDB: 6H8C, Strand ID: A, B Detail

Release date

2018-08-23

Citation

An atypical LIR motif within UBA5 (ubiquitin like modifier activating enzyme 5) interacts with GABARAP proteins and mediates membrane localization of UBA5
Huber, J., Obata, M., Gruber, J., Akutsu, M., Lohr, F., Rogova, N., Guntert, P., Dikic, I., Kirkin, V., Komatsu, M., Dotsch, V., Rogov, V.V.
Autophagy (2019), 1-15, PubMed 30990354 , DOI 10.1080/15548627.2019.1606637 , Abstract

Related entities 1. Gamma-aminobutyric acid receptor-associated protein-like 2, Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif, entity 1, : 3 : 77 entities Detail

Related entities 2. Gamma-aminobutyric acid receptor-associated protein-like 2, Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif, entity 2, : 1 : 1 : 13 entities Detail

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Bruker Avance 600 MHz - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 7.0 (±0.1), Details 0.6 mM [U-99% 13C; U-99% 15N] GABARAPL2, 1.0 mM No Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif, 50 mM No sodium phosphate, 100 mM No sodium chloride, 4.6 mM No sodium azide, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	GABARAPL2	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	natural abundance	1.0 (±0.05) mM
3	sodium phosphate	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	4.6 (±0.1) mM

2 2D 1H-15N HSQC

Instrument

Bruker Bruker Avance 600 MHz - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.2) K, pH 7.0 (±0.1), Details 1.0 mM No GABARAPL2, 0.6 mM [U-99% 13C; U-99% 15N] Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif, 50 mM No sodium phosphate, 100 mM No sodium chloride, 4.6 mM No sodium azide, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
6	GABARAPL2	natural abundance	1.0 (±0.05) mM
7	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
8	sodium phosphate	natural abundance	50 (±1.0) mM
9	sodium chloride	natural abundance	100 (±1.0) mM
10	sodium azide	natural abundance	4.6 (±0.1) mM

3 2D 1H-13C HSQC

Instrument

Bruker Bruker Avance 950 MHz - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	GABARAPL2	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	natural abundance	1.0 (±0.05) mM
3	sodium phosphate	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	4.6 (±0.1) mM

4 2D 1H-13C HSQC

Instrument

Bruker Bruker Avance 950 MHz - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
6	GABARAPL2	natural abundance	1.0 (±0.05) mM
7	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
8	sodium phosphate	natural abundance	50 (±1.0) mM
9	sodium chloride	natural abundance	100 (±1.0) mM
10	sodium azide	natural abundance	4.6 (±0.1) mM

5 3D HNCACB

Instrument

Bruker Bruker Avance 800 MHz - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	GABARAPL2	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	natural abundance	1.0 (±0.05) mM
3	sodium phosphate	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	4.6 (±0.1) mM

6 3D HNCACB

Instrument

Bruker Bruker Avance 800 MHz - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	GABARAPL2	natural abundance	1.0 (±0.05) mM
7	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
8	sodium phosphate	natural abundance	50 (±1.0) mM
9	sodium chloride	natural abundance	100 (±1.0) mM
10	sodium azide	natural abundance	4.6 (±0.1) mM

7 3D (HCA)CO(CA)NH

Instrument

Bruker Bruker Avance 700 MHz - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
6	GABARAPL2	natural abundance	1.0 (±0.05) mM
7	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
8	sodium phosphate	natural abundance	50 (±1.0) mM
9	sodium chloride	natural abundance	100 (±1.0) mM
10	sodium azide	natural abundance	4.6 (±0.1) mM

8 3D (HCA)CO(CA)NH

Instrument

Bruker Bruker Avance 700 MHz - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	GABARAPL2	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	natural abundance	1.0 (±0.05) mM
3	sodium phosphate	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	4.6 (±0.1) mM

9 3D 1H-15N NOESY

Instrument

Bruker Bruker Avance 950 MHz - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
6	GABARAPL2	natural abundance	1.0 (±0.05) mM
7	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
8	sodium phosphate	natural abundance	50 (±1.0) mM
9	sodium chloride	natural abundance	100 (±1.0) mM
10	sodium azide	natural abundance	4.6 (±0.1) mM

10 3D 1H-15N NOESY

Instrument

Bruker Bruker Avance 950 MHz - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	GABARAPL2	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	natural abundance	1.0 (±0.05) mM
3	sodium phosphate	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	4.6 (±0.1) mM

11 3D (H)CC(CO)NH-TOCSY

Instrument

Bruker Bruker Avance 900 MHz - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
6	GABARAPL2	natural abundance	1.0 (±0.05) mM
7	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
8	sodium phosphate	natural abundance	50 (±1.0) mM
9	sodium chloride	natural abundance	100 (±1.0) mM
10	sodium azide	natural abundance	4.6 (±0.1) mM

12 3D (H)CC(CO)NH-TOCSY

Instrument

Bruker Bruker Avance 900 MHz - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	GABARAPL2	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	natural abundance	1.0 (±0.05) mM
3	sodium phosphate	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	4.6 (±0.1) mM

13 3D H(CC)(CO)NH-TOCSY

Instrument

Bruker Bruker Avance 900 MHz - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
6	GABARAPL2	natural abundance	1.0 (±0.05) mM
7	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
8	sodium phosphate	natural abundance	50 (±1.0) mM
9	sodium chloride	natural abundance	100 (±1.0) mM
10	sodium azide	natural abundance	4.6 (±0.1) mM

14 3D H(CC)(CO)NH-TOCSY

Instrument

Bruker Bruker Avance 900 MHz - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	GABARAPL2	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	natural abundance	1.0 (±0.05) mM
3	sodium phosphate	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	4.6 (±0.1) mM

15 3D NOESY-[13C,1H]-HSQC 13C/15N filtered in F1

Instrument

Bruker Bruker Avance 900 MHz - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
6	GABARAPL2	natural abundance	1.0 (±0.05) mM
7	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
8	sodium phosphate	natural abundance	50 (±1.0) mM
9	sodium chloride	natural abundance	100 (±1.0) mM
10	sodium azide	natural abundance	4.6 (±0.1) mM

16 3D 1H-13C NOESY

Instrument

Bruker Bruker Avance 950 MHz - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	GABARAPL2	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	natural abundance	1.0 (±0.05) mM
3	sodium phosphate	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	4.6 (±0.1) mM

17 3D NOESY-[13C,1H]-HSQC 13C/15N filtered in F1

Instrument

Bruker Bruker Avance 900 MHz - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
1	GABARAPL2	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	natural abundance	1.0 (±0.05) mM
3	sodium phosphate	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	4.6 (±0.1) mM

18 3D 1H-13C NOESY

Instrument

Bruker Bruker Avance 950 MHz - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
6	GABARAPL2	natural abundance	1.0 (±0.05) mM
7	Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif	[U-99% 13C; U-99% 15N]	0.6 (±0.05) mM
8	sodium phosphate	natural abundance	50 (±1.0) mM
9	sodium chloride	natural abundance	100 (±1.0) mM
10	sodium azide	natural abundance	4.6 (±0.1) mM

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_34307_6h8c.nef
Input source #2: Coordindates	6h8c.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
MGWMFKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEKD
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MGWMFKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEKD

-------110------
EDGFLYVAYSGENTFG
||||||||||||||||
EDGFLYVAYSGENTFG

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

330-----340--------
GAMEIIHEDNEWGIELVSE
|||||||||||||||||||
GAMEIIHEDNEWGIELVSE
--------10---------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	116	0	0	100.0
B	B	19	0	0	100.0

Content subtype: combined_34307_6h8c.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1615		100.0 (chain: A, length: 116), 100.0 (chain: B, length: 19)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	OK	3607 (1)	noe	100.0 (chain: A, length: 116), 89.5 (chain: B, length: 19)
2	Distance restraints	DYANA/DIANA_distance_constraints_3	Warning	107 (1)	hbond	58.6 (chain: A, length: 116), 21.1 (chain: B, length: 19)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_4	Warning	253 (252)	.	99.1 (chain: A, length: 116), 94.7 (chain: B, length: 19)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1

Status: Warning

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 116, Sequence coverage: 100.0%

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
MGWMFKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEKD
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MGWMFKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEKD

-------110------
EDGFLYVAYSGENTFG
||||||||||||||||
EDGFLYVAYSGENTFG

Entity_assembly_ID: B, Chain length: 19, Sequence coverage: 100.0%

330-----340--------
GAMEIIHEDNEWGIELVSE
|||||||||||||||||||
GAMEIIHEDNEWGIELVSE

Total number of assignments
- ¹H chemical shifts: 856
- ¹³C chemical shifts: 612
- ¹⁵N chemical shifts: 147

Completeness of assignments

Entity_assemble_ID: A

Excluded Atom_ID in statistics

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
14	ARG	HH11	6.85
14	ARG	HH12	6.95
14	ARG	HH21	6.847
14	ARG	HH22	6.946
14	ARG	NH1	72.11
22	ARG	HH21	6.983
22	ARG	HH22	6.983
22	ARG	NH2	72.48
28	ARG	HH11	7.545
28	ARG	HH12	10.246
28	ARG	NH1	77.214
47	ARG	HH21	6.583
47	ARG	HH22	6.583
47	ARG	NH2	71.174
65	ARG	HH12	6.666
65	ARG	HH11	6.246
65	ARG	HH21	6.476
65	ARG	HH22	6.476
67	ARG	HH11	7.453
67	ARG	HH21	7.206
67	ARG	NH2	71.786

All atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	741	732	98.8
¹³C chemical shifts	557	530	95.2
¹⁵N chemical shifts	125	123	98.4

Backbone atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	233	228	97.9
¹³C chemical shifts	232	231	99.6
¹⁵N chemical shifts	111	109	98.2

Side chain atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	508	504	99.2
¹³C chemical shifts	325	299	92.0
¹⁵N chemical shifts	14	14	100.0

Methyl group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	67	63	94.0
¹³C chemical shifts	67	63	94.0

Aromatic group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	66	66	100.0
¹³C chemical shifts	64	42	65.6
¹⁵N chemical shifts	2	2	100.0

Entity_assemble_ID: B

Redox state of CYS
Peptide bond of PRO
Tautomeric state of HIS
Rotameric state of ILE , LEU, and VAL
Histogram of normalized assigned chemical shifts
Random coil index and derived parameters (S² and NMR RMSD)
- Chain_ID: A
- Chain_ID: B

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	109	107	98.2
¹³C chemical shifts	83	82	98.8
¹⁵N chemical shifts	21	19	90.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	40	38	95.0
¹³C chemical shifts	38	37	97.4
¹⁵N chemical shifts	19	17	89.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	69	69	100.0
¹³C chemical shifts	45	45	100.0
¹⁵N chemical shifts	2	2	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	12	12	100.0
¹³C chemical shifts	12	12	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	8	8	100.0
¹³C chemical shifts	7	7	100.0
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 116, Sequence coverage: 100.0%
  - Entity_assembly_ID: B, Chain length: 19, Sequence coverage: 89.5%
- Total number of restraints: 3606
- Weight of restraints: 1.0 (3606)
- Potential type of restraints: upper-bound-parabolic (3606)
- Range of distance target values: 2.64, 8.84 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map
- Saveframe: DYANA/DIANA_distance_constraints_3
  - Status: Warning
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 116, Sequence coverage: 58.6%
    - Entity_assembly_ID: B, Chain length: 19, Sequence coverage: 21.1%
  - Total number of restraints: 107
  - Weight of restraints: 1.0 (107)
  - Potential type of restraints: upper-bound-parabolic (107)
  - Range of distance target values: 2.1, 3.1 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_4
- Status: Warning
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 116, Sequence coverage: 99.1%
  - Entity_assembly_ID: B, Chain length: 19, Sequence coverage: 94.7%
- Total number of restraints: 252
- Total number of restraints per polymer type: 252
- Weight of restraints: 1.0 (252)
- Potential type of restraints: square-well-parabolic (252)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A
  - Chain_ID: B

Keywords GABARAPL2, SIGNALING PROTEIN, UBA5 LIR motif, protein complex

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.028 seconds)

BMRB: 34307

Sample #1

Sample #2

Related entities 1. Gamma-aminobutyric acid receptor-associated protein-like 2, Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif, entity 1, : 3 : 77 entities Detail

Related entities 2. Gamma-aminobutyric acid receptor-associated protein-like 2, Ubiquitin-like modifier-activating enzyme 5 (UBA5) LIR motif, entity 2, : 1 : 1 : 13 entities Detail

Experiments performed 18 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

NMR combined restraints 5 contents Detail