BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	90.8 % (788 of 868)	86.4 % (394 of 456)	95.2 % (315 of 331)	97.5 % (79 of 81)
Backbone	97.5 % (437 of 448)	98.1 % (153 of 156)	97.2 % (212 of 218)	97.3 % (72 of 74)
Sidechain	85.7 % (418 of 488)	80.3 % (241 of 300)	93.9 % (170 of 181)	100.0 % (7 of 7)
Aromatic	90.5 % (38 of 42)	90.5 % (19 of 21)	90.5 % (19 of 21)
Methyl	100.0 % (80 of 80)	100.0 % (40 of 40)	100.0 % (40 of 40)

1. entity 1

GDHPKYSDMI VAAIQAEKNR AGSSRQSIQK YIKSHYKVGE NADSQIKLSI KRLVTTGVLK QTKGVGASGS FRLAK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 0.925 mM [U-99% 13C; U-99% 15N] H1-GD, 10 mM Tris, 157 mM KCl, 0.1 mM EDTA, 0.125 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	H1-GD	[U-99% 13C; U-99% 15N]	0.925 mM
2	Tris	natural abundance	10 mM
3	KCl	natural abundance	157 mM
4	EDTA	natural abundance	0.1 mM
5	DSS	natural abundance	0.125 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 0.25 mM [U-99% 13C; U-99% 15N] H1-GD, 10 mM Tris, 157 mM KCl, 0.1 mM EDTA, 0.125 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	H1-GD	[U-99% 13C; U-99% 15N]	0.25 mM
7	Tris	natural abundance	10 mM
8	KCl	natural abundance	157 mM
9	EDTA	natural abundance	0.1 mM
10	DSS	natural abundance	0.125 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.32 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6HQ1, Strand ID: A Detail

Release date

2019-10-02

Citation

Structure, dynamics, and stability of the globular domain of human linker histone H1.0 and the role of positive charges
Martinsen, J.H., Saar, D., Fernandes, C.B., Schuler, B., Bugge, K., Kragelund, B.B.
Protein Sci. (2022), 31, 918-932, PubMed 35066947 , DOI 10.1002/pro.4281 , Abstract

Related entities 1. Histone H1.0, : 1 : 1 : 8 : 195 entities Detail

Interaction partners 1. Histone H1.0, : 53 interactors Detail

More details for 53 interactors identified by 16 citations

Experiments performed 13 experiments Detail

1 3D 1H-15N NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 0.925 mM [U-99% 13C; U-99% 15N] H1-GD, 10 mM Tris, 157 mM KCl, 0.1 mM EDTA, 0.125 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	H1-GD	[U-99% 13C; U-99% 15N]	0.925 mM
2	Tris	natural abundance	10 mM
3	KCl	natural abundance	157 mM
4	EDTA	natural abundance	0.1 mM
5	DSS	natural abundance	0.125 mM

2 2D 1H-15N HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 7.4, Details 0.25 mM [U-99% 13C; U-99% 15N] H1-GD, 10 mM Tris, 157 mM KCl, 0.1 mM EDTA, 0.125 mM DSS, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	H1-GD	[U-99% 13C; U-99% 15N]	0.25 mM
7	Tris	natural abundance	10 mM
8	KCl	natural abundance	157 mM
9	EDTA	natural abundance	0.1 mM
10	DSS	natural abundance	0.125 mM

3 3D HNCO

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	H1-GD	[U-99% 13C; U-99% 15N]	0.25 mM
7	Tris	natural abundance	10 mM
8	KCl	natural abundance	157 mM
9	EDTA	natural abundance	0.1 mM
10	DSS	natural abundance	0.125 mM

4 3D HNCACB

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	H1-GD	[U-99% 13C; U-99% 15N]	0.25 mM
7	Tris	natural abundance	10 mM
8	KCl	natural abundance	157 mM
9	EDTA	natural abundance	0.1 mM
10	DSS	natural abundance	0.125 mM

5 3D HN(CO)CA

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	H1-GD	[U-99% 13C; U-99% 15N]	0.25 mM
7	Tris	natural abundance	10 mM
8	KCl	natural abundance	157 mM
9	EDTA	natural abundance	0.1 mM
10	DSS	natural abundance	0.125 mM

6 3D CBCA(CO)NH

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	H1-GD	[U-99% 13C; U-99% 15N]	0.25 mM
7	Tris	natural abundance	10 mM
8	KCl	natural abundance	157 mM
9	EDTA	natural abundance	0.1 mM
10	DSS	natural abundance	0.125 mM

7 3D HNCA

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
6	H1-GD	[U-99% 13C; U-99% 15N]	0.25 mM
7	Tris	natural abundance	10 mM
8	KCl	natural abundance	157 mM
9	EDTA	natural abundance	0.1 mM
10	DSS	natural abundance	0.125 mM

8 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 750 MHz Cryoprobe

Sample

#	Name	Isotope labeling	Concentration
1	H1-GD	[U-99% 13C; U-99% 15N]	0.925 mM
2	Tris	natural abundance	10 mM
3	KCl	natural abundance	157 mM
4	EDTA	natural abundance	0.1 mM
5	DSS	natural abundance	0.125 mM

9 3D HNCACB

Instrument

Bruker AVANCE III - 750 MHz Cryoprobe

Sample

#	Name	Isotope labeling	Concentration
1	H1-GD	[U-99% 13C; U-99% 15N]	0.925 mM
2	Tris	natural abundance	10 mM
3	KCl	natural abundance	157 mM
4	EDTA	natural abundance	0.1 mM
5	DSS	natural abundance	0.125 mM

10 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE III - 750 MHz Cryoprobe

Sample

#	Name	Isotope labeling	Concentration
1	H1-GD	[U-99% 13C; U-99% 15N]	0.925 mM
2	Tris	natural abundance	10 mM
3	KCl	natural abundance	157 mM
4	EDTA	natural abundance	0.1 mM
5	DSS	natural abundance	0.125 mM

11 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE III - 750 MHz Cryoprobe

Sample

#	Name	Isotope labeling	Concentration
1	H1-GD	[U-99% 13C; U-99% 15N]	0.925 mM
2	Tris	natural abundance	10 mM
3	KCl	natural abundance	157 mM
4	EDTA	natural abundance	0.1 mM
5	DSS	natural abundance	0.125 mM

12 3D HCCH-TOCSY

Instrument

Varian INOVA - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	H1-GD	[U-99% 13C; U-99% 15N]	0.925 mM
2	Tris	natural abundance	10 mM
3	KCl	natural abundance	157 mM
4	EDTA	natural abundance	0.1 mM
5	DSS	natural abundance	0.125 mM

13 3D 1H-15N TOCSY

Instrument

Bruker AVANCE III - 750 MHz Cryoprobe

Sample

#	Name	Isotope labeling	Concentration
1	H1-GD	[U-99% 13C; U-99% 15N]	0.925 mM
2	Tris	natural abundance	10 mM
3	KCl	natural abundance	157 mM
4	EDTA	natural abundance	0.1 mM
5	DSS	natural abundance	0.125 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34318_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34318_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	852		100.0 (chain: 1, length: 75)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 75, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 463
  - ¹³C chemical shifts: 311
  - ¹⁵N chemical shifts: 78
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	456	385	84.4
¹³C chemical shifts	331	311	94.0
¹⁵N chemical shifts	85	78	91.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	156	152	97.4
¹³C chemical shifts	150	145	96.7
¹⁵N chemical shifts	74	71	95.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	300	233	77.7
¹³C chemical shifts	181	166	91.7
¹⁵N chemical shifts	11	7	63.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	41	39	95.1
¹³C chemical shifts	41	38	92.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	21	19	90.5
¹³C chemical shifts	21	19	90.5

Keywords DNA BINDING PROTEIN, alpha-helical, nucleosome assembly

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Link destinations for BMRB, PDB and EMDB

Found 1 Document (3.004 seconds)

BMRB: 34318

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Histone H1.0, : 1 : 1 : 8 : 195 entities Detail

Interaction partners 1. Histone H1.0, : 53 interactors Detail

Experiments performed 13 experiments Detail

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Chemical shift validation 3 contents Detail