BMRB: 34320

Pepducin UT-Pep2 a biased allosteric agonist of Urotensin-II receptor

Authors

Carotenuto, A., Hoang, T.A., Nassour, H., Martin, R.D., Billard, E., Myriam, L., Novellino, E., Tanny, J.C., Fournier, A., Hebert, T.E., Chatenet, D.

Assembly

Urotensin-2 receptor

Entity

1. Urotensin-2 receptor (polymer, Thiol state: not present), 14 monomers, 1453.668 Da Detail

XRPLDTVQRP KGYX

Formula weight

1453.668 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 85.7 %, Completeness: 98.8 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	98.8 % (80 of 81)	98.8 % (80 of 81)
Backbone	100.0 % (23 of 23)	100.0 % (23 of 23)
Sidechain	98.3 % (57 of 58)	98.3 % (57 of 58)
Aromatic	100.0 % (4 of 4)	100.0 % (4 of 4)
Methyl	100.0 % (5 of 5)	100.0 % (5 of 5)

1. entity 1

XRPLDTVQRP KGYX

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.5, Details 2.0 mM 1H peptide, 200 mM [U-99% 2H] DPC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	peptide	natural abundance		2.0 mM
2	DPC	[U-99% 2H]		200 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 6HVK, Strand ID: A Detail

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Release date

2019-10-21

Citation

Lipidated peptides derived from intracellular loops 2 and 3 of the urotensin II receptor act as biased allosteric ligands
Nassour, H., Hoang, T.A., Martin, R.D., Dallagnol, J., Billard, E., Letourneau, M., Novellino, E., Carotenuto, A., Allen, B.G., Tanny, J.C., Fournier, A., Hebert, T.E., Chatenet, D.
J. Biol. Chem. (2021), 297, 101057-101057, PubMed 34389356 , DOI 10.1016/j.jbc.2021.101057 , Abstract

Related entities 1. Urotensin-2 receptor, : 1 : 4 : 5 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail