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NMR solution structure of the C/D box snoRNA U14
Authors
Chagot, M.E., Quinternet, M., Rothe, B., Charpentier, B., Coutant, J., Manival, X., Lebars, I.
Assembly
RNA (31-MER)
Entity
1. RNA (31-MER) (polymer, Thiol state: not present), 31 monomers, 10061.92 Da Detail

GGCACGGUGA UGACCUUCGG GUCUGAGUGC C


Formula weight
10061.92 Da
Source organism
synthetic construct
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 87.6 %, Completeness (bb): 85.2 % Detail

Polymer type: polyribonucleotide

Total1H13C15N31P
All87.6 % (461 of 526)89.3 % (242 of 271)93.7 % (192 of 205)84.2 % (16 of 19)35.5 % (11 of 31)
Suger, PO485.2 % (317 of 372)88.2 % (164 of 186)91.6 % (142 of 155)35.5 % (11 of 31)
Nucleobase93.5 % (144 of 154)91.8 % (78 of 85)100.0 % (50 of 50)84.2 % (16 of 19)
Aromatic94.2 % (130 of 138)92.8 % (64 of 69)100.0 % (50 of 50)84.2 % (16 of 19)

1. entity 1

GGCACGGUGA UGACCUUCGG GUCUGAGUGC C

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4, Details 500 uM RNA (31-MER), 500 uM [U-99% 13C; U-99% 15N] RNA (31-MER), 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
1RNA (31-MER)_unlabelnatural abundance500 uM
2RNA (31-MER)_label[U-99% 13C; U-99% 15N]500 uM
3NaClnatural abundance150 mM
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4, Details 500 uM RNA (31-MER), 500 uM [U-99% 13C; U-99% 15N] RNA (31-MER), 100% D2O


#NameIsotope labelingTypeConcentration
4RNA (31-MER)_unlabelnatural abundance500 uM
5RNA (31-MER)_label[U-99% 13C; U-99% 15N]500 uM
6NaClnatural abundance150 mM

Release date
2019-04-17
Citation
The yeast C/D box snoRNA U14 adopts a "weak" K-turn like conformation recognized by the Snu13 core protein in solution
Chagot, M.E., Quinternet, M., Rothe, B., Charpentier, B., Coutant, J., Manival, X., Lebars, I.
Biochimie (2019), 164, 70-82, PubMed 30914254 , DOI 10.1016/j.biochi.2019.03.014 ,
Related entities 1. RNA (31-MER), : 1 entities Detail
Experiments performed 27 experiments Detail
NMR combined restraints 6 contents Detail
Keywords C/D box snoRNA, K-turn, RNA, Snu13p protein, U14