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BMRB: 34326

6I3R

Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1/2 of Drosophila helicase MLE

Authors

Jagtap, P.K.A., Buelow, S.V., Masiewicz, P., Simon, B., Hennig, J.

Assembly

Dosage compensation regulator (E.C.3.6.4.13)

Entity

1. Dosage compensation regulator (E.C.3.6.4.13) (polymer), 259 monomers, 28548.56 Da Detail

GAMDIKSFLY QFCAKSQIEP KFDIRQTGPK NRQRFLCEVR VEPNTYIGVG NSTNKKDAEK NACRDFVNYL VRVGKLNTND VPADAGASGG GPRTGLEGAG MAGGSGQQKR VFDGQSGPQD LGEAYRPLNH DGGDGGNRYS VIDRIQEQRD MNEAEAFDVN AAIHGNWTIE NAKERLNIYK QTNNIRDDYK YTPVGPEHAR SFLAELSIYV PALNRTVTAR ESGSNKKSAS KSCALSLVRQ LFHLNVIEPF SGTLKKKKD

Formula weight

28548.56 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 99.6 %, Completeness: 91.1 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.1 % (2693 of 2956)	89.9 % (1395 of 1552)	91.0 % (1022 of 1123)	98.2 % (276 of 281)
Backbone	96.9 % (1485 of 1532)	95.5 % (509 of 533)	97.2 % (730 of 751)	99.2 % (246 of 248)
Sidechain	86.8 % (1439 of 1657)	86.9 % (886 of 1019)	86.4 % (523 of 605)	90.9 % (30 of 33)
Aromatic	51.5 % (103 of 200)	65.0 % (65 of 100)	37.4 % (37 of 99)	100.0 % (1 of 1)
Methyl	94.5 % (223 of 236)	91.5 % (108 of 118)	97.5 % (115 of 118)

1. entity 1

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 298 K, pH 6, Details 0.5 mM [U-13C; U-15N] MLE dsRBD1,2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	MLE dsRBD1,2	[U-13C; U-15N]		0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 6 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 6I3R, Strand ID: A Detail

Release date

2019-03-12

Citation

Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless
Ankush Jagtap, P.K., Muller, M., Masiewicz, P., von Bulow, S., Hollmann, N.M., Chen, P.C., Simon, B., Thomae, A.W., Becker, P.B., Hennig, J.
Nucleic Acids Res. (2019), 47, 4319-4333, PubMed 30805612 , DOI 10.1093/nar/gkz125 , Abstract

Related entities 1. Dosage compensation regulator (E.C.3.6.4.13), : 1 : 4 : 11 entities Detail

Interaction partners 1. Dosage compensation regulator (E.C.3.6.4.13), : 9 interactors Detail

More details for 9 interactors identified by 2 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC