BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	95.9 % (1450 of 1512)	96.1 % (765 of 796)	95.0 % (548 of 577)	98.6 % (137 of 139)
Backbone	97.3 % (726 of 746)	96.0 % (242 of 252)	97.6 % (362 of 371)	99.2 % (122 of 123)
Sidechain	93.9 % (833 of 887)	95.0 % (517 of 544)	92.0 % (301 of 327)	93.8 % (15 of 16)
Aromatic	69.8 % (60 of 86)	88.4 % (38 of 43)	51.2 % (22 of 43)
Methyl	98.6 % (138 of 140)	97.1 % (68 of 70)	100.0 % (70 of 70)

1. entity 1

GPHMTFKALK EEGNQCVNDK NYKDALSKYS ECLKINNKEC AIYTNRALCY LKLCQFEEAK QDCDQALQLA DGNVKAFYRR ALAHKGLKNY QKSLIDLNKV ILLDPSIIEA KMELEEVTRL LNLKD

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR3, 150 mM sodium chloride, 10 mM sodium phosphate, 0.5 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SPAG1-TPR3	[U-13C; U-15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	TCEP	natural abundance	0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 3.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 3.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 2.98 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6I57, Strand ID: A Detail

Release date

2019-05-27

Citation

Binding properties of the quaternary assembly protein SPAG1
Chagot, M.E., Dos Santos Morais, R., Dermouche, S., Lefebvre, D., Manival, X., Chipot, C., Dehez, F., Quinternet, M.
Biochem. J. (2019), 476, 1679-1694, PubMed 31118266 , DOI 10.1042/BCJ20190198 , Abstract

Related entities 1. Sperm-associated antigen 1, : 1 : 2 : 234 entities Detail

Interaction partners 1. Sperm-associated antigen 1, : 7 interactors Detail

More details for 7 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.4, Details 1 mM [U-13C; U-15N] SPAG1-TPR3, 150 mM sodium chloride, 10 mM sodium phosphate, 0.5 mM TCEP, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	SPAG1-TPR3	[U-13C; U-15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	TCEP	natural abundance	0.5 mM

2 3D HNCA

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SPAG1-TPR3	[U-13C; U-15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	TCEP	natural abundance	0.5 mM

3 3D HNCACB

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SPAG1-TPR3	[U-13C; U-15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	TCEP	natural abundance	0.5 mM

4 3D HBHA(CO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SPAG1-TPR3	[U-13C; U-15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	TCEP	natural abundance	0.5 mM

5 3D H(CCO)NH

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SPAG1-TPR3	[U-13C; U-15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	TCEP	natural abundance	0.5 mM

6 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SPAG1-TPR3	[U-13C; U-15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	TCEP	natural abundance	0.5 mM

7 3D HNHA

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SPAG1-TPR3	[U-13C; U-15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	TCEP	natural abundance	0.5 mM

8 3D HCCH-TOCSY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SPAG1-TPR3	[U-13C; U-15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	TCEP	natural abundance	0.5 mM

9 3D HNCO

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SPAG1-TPR3	[U-13C; U-15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	TCEP	natural abundance	0.5 mM

10 2D 1H-1H NOESY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SPAG1-TPR3	[U-13C; U-15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	TCEP	natural abundance	0.5 mM

11 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SPAG1-TPR3	[U-13C; U-15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	TCEP	natural abundance	0.5 mM

12 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SPAG1-TPR3	[U-13C; U-15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	TCEP	natural abundance	0.5 mM

13 3D 1H-13C NOESY

Instrument

Bruker AvanceIII - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	SPAG1-TPR3	[U-13C; U-15N]	1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	TCEP	natural abundance	0.5 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34329_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34329_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1638		100.0 (chain: 1, length: 125)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 125, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 940
  - ¹³C chemical shifts: 555
  - ¹⁵N chemical shifts: 143
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
3	HIS	ND1	199.195
3	HIS	NE2	176.49
32	CYS	HG	1.876
49	CYS	HG	2.855
50	TYR	HH	11.325
63	CYS	HG	2.698
78	TYR	HH	10.202
93	SER	HG	4.049
118	THR	HG1	4.979

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	796	789	99.1
¹³C chemical shifts	577	555	96.2
¹⁵N chemical shifts	143	141	98.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	252	251	99.6
¹³C chemical shifts	250	249	99.6
¹⁵N chemical shifts	123	122	99.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	544	538	98.9
¹³C chemical shifts	327	306	93.6
¹⁵N chemical shifts	20	19	95.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	72	72	100.0
¹³C chemical shifts	72	72	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	43	43	100.0
¹³C chemical shifts	43	24	55.8

Keywords ATPase, CHAPERONE, EEVD, GTPase, HSP70, HSP90, MD simulation, R2SP, R2TP, RUVBL, assembly factor, cilia, dynein

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Found 1 Document (0.799 seconds)

BMRB: 34329

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Sperm-associated antigen 1, : 1 : 2 : 234 entities Detail

Interaction partners 1. Sperm-associated antigen 1, : 7 interactors Detail

Experiments performed 13 experiments Detail

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Chemical shift validation 3 contents Detail