BMRB: 34358

NMR solution structure of LSR2 binding domain.

Authors

Barthe, P., Cohen-Gonsaud, M., Mukamolova, G.V.

Assembly

Nucleoid-associated protein Lsr2

Entity

1. Nucleoid-associated protein Lsr2 (polymer, Thiol state: not present), 50 monomers, 5385.831 Da Detail

GHMSGSGRGR GAIDREQSAA IREWARRNGH NVSTRGRIPA DVIDAYHAAT

Formula weight

5385.831 Da

Source organism

Mycobacterium tuberculosis H37Rv

Exptl. method

solution NMR

Refine. method

MDSA and refinement in explicit water

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.0 %, Completeness: 94.2 %, Completeness (bb): 94.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	94.2 % (307 of 326)	94.1 % (257 of 273)	94.3 % (50 of 53)
Backbone	94.2 % (146 of 155)	94.3 % (100 of 106)	93.9 % (46 of 49)
Sidechain	94.2 % (161 of 171)	94.0 % (157 of 167)	100.0 % (4 of 4)
Aromatic	88.2 % (15 of 17)	87.5 % (14 of 16)	100.0 % (1 of 1)
Methyl	100.0 % (22 of 22)	100.0 % (22 of 22)

1. entity 1

GHMSGSGRGR GAIDREQSAA IREWARRNGH NVSTRGRIPA DVIDAYHAAT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 bar, Temperature 293 K, pH 6.8, Details 0.5 mM [U-15N] LSR2 protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	LSR2 protein	[U-15N]		0.5 mM

Sample #2

Solvent system 100% D2O, Pressure 1 bar, Temperature 293 K, pH 6.8, Details 0.5 mM [U-15N] LSR2 protein, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	LSR2 protein	[U-15N]		0.5 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 6QKP, Strand ID: A Detail

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Release date

2019-09-26

Citation

Protein kinase B controls Mycobacterium tuberculosis growth via phosphorylation of the transcriptional regulator Lsr2 at threonine 112
Alqaseer, K., Turapov, O., Barthe, P., Jagatia, H., De Visch, A., Roumestand, C., Wegrzyn, M., Bartek, I.L., Voskuil, M.I., O'Hare, H.M., Ajuh, P., Bottrill, A.R., Witney, A.A., Cohen-Gonsaud, M., Waddell, S.J., Mukamolova, G.V.
Mol. Microbiol. (2019), 112, 1847-1862, PubMed 31562654 , DOI 10.1111/mmi.14398 , Abstract

Related entities 1. Nucleoid-associated protein Lsr2, : 1 : 2 : 6 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail