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BMRB: 34362

tSH2 domain of transcription elongation factor Spt6 complexed with tyrosine phosphorylated CTD

Authors

Brazda, P., Krejcikova, M., Smirakova, E., Kubicek, K., Stefl, R.

Assembly

tSH2 domain of transcription elongation factor Spt6, Tyrosine phosphorylated CTD

Entity

1. tSH2 domain of transcription elongation factor Spt6 (polymer), 196 monomers, 23223.18 Da Detail

SHRVINHPYY FPFNGKQAED YLRSKERGDF VIRQSSRGDD HLAITWKLDK DLFQHVDIQE LEKENPLALG KVLVVEGQRY HDLDQIIVEY LQNKIRLLNE LTSNEKFKAG TKKEVVKFIE DYSKVNPKKS VYYFSLNYEN PGWFYLIFKL NAESKLYIWN VKLTHTGFFL VNYNYPTVIQ LCNGFKTLLK SSNTRN

2. Tyrosine phosphorylated CTD (polymer), 16 monomers, 1801.646 Da Detail

PSXSPTSPSX SPTSPS

Total weight

25024.826 Da

Max. entity weight

23223.18 Da

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.4 %, Completeness: 63.7 %, Completeness (bb): 68.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	63.7 % (1659 of 2606)	68.0 % (928 of 1364)	55.4 % (563 of 1016)	74.3 % (168 of 226)
Backbone	68.2 % (844 of 1238)	79.2 % (331 of 418)	56.0 % (348 of 621)	82.9 % (165 of 199)
Sidechain	61.6 % (966 of 1569)	62.9 % (595 of 946)	61.7 % (368 of 596)	11.1 % (3 of 27)
Aromatic	22.5 % (64 of 284)	28.9 % (41 of 142)	16.5 % (23 of 139)	0.0 % (0 of 3)
Methyl	87.2 % (190 of 218)	86.2 % (94 of 109)	88.1 % (96 of 109)

1. tSH2 domain of transcription elongation factor Spt6

2. Tyrosine phosphorylated CTD

PSXSPTSPSX SPTSPS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 310 (±0.05) K, pH 7 (±0.1), Details 0.5 mM [U-99% 15N] spt6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	spt6	[U-99% 15N]		0.5 (±0.05) mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 310 (±0.05) K, pH 7 (±0.1), Details 0.5 mM [U-99% 13C; U-99% 15N] spt6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
2	spt6	[U-99% 13C; U-99% 15N]		0.5 (±0.05) mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 310 (±0.05) K, pH 7 (±0.1), Details 0.5 mM spt6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	spt6	natural abundance		0.5 (±0.05) mM

Protein Blocks Logo

Calculated from 20 models in PDB: 6QTC, Strand ID: A, B Detail

Release date

2019-10-21

Citation

Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro
Brazda, P., Krejcikova, M., Kasiliauskaite, A., Smirakova, E., Klumpler, T., Vacha, R., Kubicek, K., Stefl, R.
J. Mol. Biol. (2020), 432, 4092-4107, PubMed 32439331 , DOI 10.1016/j.jmb.2020.05.007 , Abstract

Related entities 1. tSH2 domain of transcription elongation factor Spt6, : 1 : 28 entities Detail

Related entities 2. Tyrosine phosphorylated CTD, : 1 : 1 entities Detail

Interaction partners 1. tSH2 domain of transcription elongation factor Spt6, : 27 interactors Detail

More details for 27 interactors identified by 10 citations

Experiments performed 7 experiments Detail

1 3D HNCA

Instrument

Bruker AvanceIII - 700 MHz cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 310 (±0.05) K, pH 7 (±0.1), Details 0.5 mM [U-99% 15N] spt6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	spt6	[U-99% 15N]		0.5 (±0.05) mM

2 3D HN(CO)CA