BMRB: 34371

Solution NMR of synthetic analogues of nisin and mutacin ring A and ring B - Mutacin I Ring A truncated analogue

Authors

Dickman, R., Mitchell, S.A., Figueiredo, A., Hansen, D.F., Tabor, A.B.

Assembly

DAL-PRO-GLY-CYS-LYS

Entity

1. DAL-PRO-GLY-CYS-LYS (polymer), 6 monomers, 576.7066 Da Detail

XLSLCA

Formula weight

576.7066 Da

Source organism

Lactococcus lactis

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.3 %, Completeness: 54.3 %, Completeness (bb): 100.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C
All	54.3 % (25 of 46)	40.0 % (10 of 25)	71.4 % (15 of 21)
Backbone	100.0 % (25 of 25)	100.0 % (10 of 10)	100.0 % (15 of 15)
Sidechain	19.2 % (5 of 26)	0.0 % (0 of 15)	45.5 % (5 of 11)
Methyl	10.0 % (1 of 10)	0.0 % (0 of 5)	20.0 % (1 of 5)

1. entity 1

XLSLCA

Show sample condition

Sample

Solvent system DMSO, Pressure 1 atm, Temperature 298 K, pH 5.0, Details 5.5 mg/mL Mutacin I Ring A truncated analogue, DMSO

#	Name	Isotope labeling	Type	Concentration
1	Mutacin I Ring A truncated analogue	natural abundance		5.5 mg/mL

Hide sample codition

Protein Blocks Logo

Calculated from 15 models in PDB: 6QYT, Strand ID: A Detail

---

Release date

2019-09-26

Citation 1

A chemical biology approach to understanding molecular recognition of lipid II by nisin: Solid-phase synthesis and NMR ensemble analysis of nisin(1-12) and a synthetic ana-logue
Dickman, R., Danelius, E., Mitchell, S.A., Hansen, D.F., Erdelyi, M., Tabor, A.B.

Show more 1 citaion

Citation 2

Molecular Recognition of Lipid II by Lantibiotics: Synthesis and Conformational Studies of Analogues of Nisin and Mutacin Rings A and B
Dickman, R., Mitchell, S.A., Figueiredo, A.M., Hansen, D.F., Tabor, A.B.
J. Org. Chem. (2019), 84, 11493-11512, PubMed 31464129 , DOI 10.1021/acs.joc.9b01253 , Abstract

Hide non-primary 1 citaion

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail