BMRB: 34400

RW16 peptide

Authors

Jobin, M.L., Grelard, A., Alves, I., Mackereth, C.D.

Assembly

ARG-ARG-TRP-ARG-ARG-TRP-TRP-ARG-ARG-TRP-TRP-ARG-ARG-TRP-ARG-ARG

Entity

1. ARG-ARG-TRP-ARG-ARG-TRP-TRP-ARG-ARG-TRP-TRP-ARG-ARG-TRP-ARG-ARG (polymer, Thiol state: not present), 18 monomers, 3022.558 Da Detail

XXRRWRRWWR RWWRRWRR

Formula weight

3022.558 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Refine. method

simulated annealingsimulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 88.9 %, Completeness: 71.0 %, Completeness (bb): 40.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	71.0 % (176 of 248)	97.3 % (146 of 150)	30.6 % (30 of 98)
Backbone	40.0 % (32 of 80)	100.0 % (32 of 32)	0.0 % (0 of 48)
Sidechain	78.3 % (144 of 184)	96.6 % (114 of 118)	45.5 % (30 of 66)
Aromatic	100.0 % (66 of 66)	100.0 % (36 of 36)	100.0 % (30 of 30)

1. entity 1

XXRRWRRWWR RWWRRWRR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 5.5, Details 1 mM RW16 peptide, 60 mM [U-100% 2H] DPC, 50 mM sodium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RW16 peptide	natural abundance		1 mM
2	DPC	[U-100% 2H]		60 mM
3	sodium phosphate	natural abundance		50 mM

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Calculated from 10 models in PDB: 6RQS, Strand ID: A Detail

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Release date

2019-10-16

Citation

Biophysical Insight on the Membrane Insertion of an Arginine-Rich Cell-Penetrating Peptide
Jobin, M.L., Vamparys, L., Deniau, R., Grelard, A., Mackereth, C.D., Fuchs, P.F.J., Alves, I.D.
Int. J. Mol. Sci. (2019), 20, E4441-E4441, PubMed 31505894 , DOI 10.3390/ijms20184441 , Abstract

Related entities 1. ARG-ARG-TRP-ARG-ARG-TRP-TRP-ARG-ARG-TRP-TRP-ARG-ARG-TRP-ARG-ARG, : 7 entities Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail