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BMRB: 34421

6SDW

Solution structure of Staufen1 dsRBD3+4 - hARF1 SBS dsRNA complex.

Authors

Yadav, D.K., Lukavsky, P.J.

Assembly

Double-stranded RNA-binding protein Staufen homolog 1/RNA Complex

Entity

1. Double-stranded RNA-binding protein Staufen homolog 1/RNA Complex, entity 1 (polymer, Thiol state: not present), 177 monomers, 19732.80 Da Detail

GSHMKSEISQ VFEIALKRNL PVNFEVARES GPPHMKNFVT KVSVGEFVGE GEGKSKKISK KNAAIAVLEE LKKLPPLPAV ERVKPRIKKK TKPIVKPQTS PEYGQGINPI SRLAQIQQAK KEKEPEYTLL TERGLPRRRE FVMQVKVGNH TAEGTGTNKK VAKRNAAENM LEILGFK

2. Double-stranded RNA-binding protein Staufen homolog 1/RNA Complex, entity 2 (polymer, Thiol state: not present), 34 monomers, 10962.45 Da Detail

GGCAGAAGCU GCCUCUUCGG AGGCAGUUUC UGCC

Total weight

30695.25 Da

Max. entity weight

19732.8 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealingna

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.5 %, Completeness: 84.2 %, Completeness (bb): 89.5 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	84.2 % (2265 of 2690)	85.1 % (1228 of 1443)	83.9 % (879 of 1048)	79.4 % (158 of 199)
Backbone	89.5 % (1262 of 1410)	90.7 % (506 of 558)	89.0 % (612 of 688)	87.8 % (144 of 164)
Sidechain	79.5 % (1148 of 1444)	81.6 % (722 of 885)	78.6 % (412 of 524)	40.0 % (14 of 35)
Aromatic	59.6 % (143 of 240)	80.8 % (97 of 120)	45.5 % (46 of 101)	0.0 % (0 of 19)
Methyl	91.0 % (171 of 188)	90.4 % (85 of 94)	91.5 % (86 of 94)

1. entity 1

GSHMKSEISQ VFEIALKRNL PVNFEVARES GPPHMKNFVT KVSVGEFVGE GEGKSKKISK KNAAIAVLEE LKKLPPLPAV ERVKPRIKKK TKPIVKPQTS PEYGQGINPI SRLAQIQQAK KEKEPEYTLL TERGLPRRRE FVMQVKVGNH TAEGTGTNKK VAKRNAAENM LEILGFK

2. entity 2

GGCAGAAGCU GCCUCUUCGG AGGCAGUUUC UGCC

Show sample condition

Sample #1

Solvent system 90-95% H2O/5-10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 310 uM [U-15N] Staufen1 dsRBD4, 310 uM Unlabelled hARF1, 90-95% H2O/5-10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Staufen1 dsRBD4	[U-15N]		310 uM
2	hARF1	Unlabelled		310 uM

Sample #2

Solvent system 90-95% H2O/5-10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 405 uM [U-13C; U-15N] Staufen1 dsRBD4, 405 uM Unlabelled hARF1, 90-95% H2O/5-10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Staufen1 dsRBD4	[U-13C; U-15N]		405 uM
4	hARF1	Unlabelled		405 uM

Sample #3

Solvent system 90-95% H2O/5-10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 310 uM Unlabelled hARF1, 90-95% H2O/5-10% D2O

#	Name	Isotope labeling	Type	Concentration
5	hARF1	Unlabelled		310 uM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 478 uM [U-13C] hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
6	hARF1	[U-13C]		478 uM

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 354 uM [U-15N] Staufen1 dsRBD4, 354 uM [U-13C] hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	Staufen1 dsRBD4	[U-15N]		354 uM
8	hARF1	[U-13C]		354 uM

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 451 uM [U-13C; U-15N] Staufen1 dsRDB4, 451 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
9	Staufen1 dsRDB4	[U-13C; U-15N]		451 uM
10	hARF1	Unlabelled		451 uM

Sample #7

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 310 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
11	hARF1	Unlabelled		310 uM

Sample #8

Solvent system 90-95% H2O/5-10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 417 uM [U-15N] Staufen1 dsRBD3, 417 uM Unlabelled hARF1, 90-95% H2O/5-10% D2O

#	Name	Isotope labeling	Type	Concentration
12	Staufen1 dsRBD3	[U-15N]		417 uM
13	hARF1	Unlabelled		417 uM

Sample #9

Solvent system 90-95% H2O/5-10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 458 uM [U-13C; U-15N] Staufen1 dsRBD3, 458 uM Unlabelled hARF1, 90-95% H2O/5-10% D2O

#	Name	Isotope labeling	Type	Concentration
14	hARF1	Unlabelled		458 uM
15	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

Sample #10

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 458 uM [U-13C; U-15N] Staufen1 dsRBD3, 458 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
16	hARF1	Unlabelled		458 uM
17	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

Sample #11

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 157 uM [U-13C; U-15N] Staufen1 dsRBD3, 157 uM Unlabelled Staufen1 dsRBD4, 157 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Concentration
18	Staufen1 dsRBD4	Unlabelled	157 uM
19	hARF1	Unlabelled	157 uM
20	Staufen1 dsRBD3	[U-13C; U-15N]	157 uM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	na	indirect	0.2514495
¹H	DSS	methyl protons	na	indirect	1.0
¹⁵N	DSS	nitrogen	na	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	na	indirect	0.2514495
¹H	DSS	methyl protons	na	indirect	1.0
¹⁵N	DSS	nitrogen	na	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	na	indirect	0.2514495
¹H	DSS	methyl protons	na	indirect	1.0
¹⁵N	DSS	nitrogen	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbons	na	indirect	0.2514495
¹H	DSS	methyl protons	na	indirect	1.0
¹⁵N	DSS	nitrogen	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 6SDW, Strand ID: A Detail

Release date

2020-01-06

Citation

Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition
Yadav, D.K., Zigackova, D., Zlobina, M., Klumpler, T., Beaumont, C., Kubickova, M., Vanacova, S., Lukavsky, P.J.
Nucleic Acids Res. (2019), 48, 2091-2106, PubMed 31875226 , DOI 10.1093/nar/gkz1163 , Abstract

Related entities 1. Double-stranded RNA-binding protein Staufen homolog 1/RNA Complex, entity 1, : 1 : 2 : 122 entities Detail

Experiments performed 59 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90-95% H2O/5-10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 310 uM [U-15N] Staufen1 dsRBD4, 310 uM Unlabelled hARF1, 90-95% H2O/5-10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Staufen1 dsRBD4	[U-15N]		310 uM
2	hARF1	Unlabelled		310 uM

2 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Staufen1 dsRBD4	[U-15N]		310 uM
2	hARF1	Unlabelled		310 uM

3 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Staufen1 dsRBD4	[U-15N]		310 uM
2	hARF1	Unlabelled		310 uM

4 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90-95% H2O/5-10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 405 uM [U-13C; U-15N] Staufen1 dsRBD4, 405 uM Unlabelled hARF1, 90-95% H2O/5-10% D2O

#	Name	Isotope labeling	Type	Concentration
3	Staufen1 dsRBD4	[U-13C; U-15N]		405 uM
4	hARF1	Unlabelled		405 uM

5 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	Staufen1 dsRBD4	[U-13C; U-15N]		405 uM
4	hARF1	Unlabelled		405 uM

6 3D HNCA

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	Staufen1 dsRBD4	[U-13C; U-15N]		405 uM
4	hARF1	Unlabelled		405 uM

7 3D HNCO

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	Staufen1 dsRBD4	[U-13C; U-15N]		405 uM
4	hARF1	Unlabelled		405 uM

8 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	Staufen1 dsRBD4	[U-13C; U-15N]		405 uM
4	hARF1	Unlabelled		405 uM

9 3D HNCACB

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	Staufen1 dsRBD4	[U-13C; U-15N]		405 uM
4	hARF1	Unlabelled		405 uM

10 3D HBHA(CO)NH

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	Staufen1 dsRBD4	[U-13C; U-15N]		405 uM
4	hARF1	Unlabelled		405 uM

11 3D HcccoNH-TOCSY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	Staufen1 dsRBD4	[U-13C; U-15N]		405 uM
4	hARF1	Unlabelled		405 uM

12 3D CccoNH-TOCSY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	Staufen1 dsRBD4	[U-13C; U-15N]		405 uM
4	hARF1	Unlabelled		405 uM

13 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	Staufen1 dsRBD4	[U-13C; U-15N]		405 uM
4	hARF1	Unlabelled		405 uM

14 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	Staufen1 dsRBD4	[U-13C; U-15N]		405 uM
4	hARF1	Unlabelled		405 uM

15 3D 1H-15N NOESY

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Staufen1 dsRBD4	[U-15N]		310 uM
2	hARF1	Unlabelled		310 uM

16 2D 1H-1H, 13C 2F-filtered NOESY

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 451 uM [U-13C; U-15N] Staufen1 dsRDB4, 451 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
9	Staufen1 dsRDB4	[U-13C; U-15N]		451 uM
10	hARF1	Unlabelled		451 uM

17 3D 13C 1F-filtered 2F-edited HSQC-NOESY

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 451 uM [U-13C; U-15N] Staufen1 dsRDB4, 451 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
9	Staufen1 dsRDB4	[U-13C; U-15N]		451 uM
10	hARF1	Unlabelled		451 uM

18 3D 1H-13C NOESY ribose

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 354 uM [U-15N] Staufen1 dsRBD4, 354 uM [U-13C] hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	Staufen1 dsRBD4	[U-15N]		354 uM
8	hARF1	[U-13C]		354 uM

19 2D 1H-1H TOCSY

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 90-95% H2O/5-10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 310 uM Unlabelled hARF1, 90-95% H2O/5-10% D2O

#	Name	Isotope labeling	Type	Concentration
5	hARF1	Unlabelled		310 uM

20 2D 1H-1H NOESY

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 90-95% H2O/5-10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 310 uM Unlabelled hARF1, 90-95% H2O/5-10% D2O

#	Name	Isotope labeling	Type	Concentration
5	hARF1	Unlabelled		310 uM

21 2D 1H-13C HSQC

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 478 uM [U-13C] hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
6	hARF1	[U-13C]		478 uM

22 3D HCCH-TOCSY

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 478 uM [U-13C] hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
6	hARF1	[U-13C]		478 uM

23 3D 1H-13C NOESY

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 478 uM [U-13C] hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
6	hARF1	[U-13C]		478 uM

24 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 478 uM [U-13C] hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
6	hARF1	[U-13C]		478 uM

25 2D 13C 1F-filtered 2F-filtered NOESY

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 451 uM [U-13C; U-15N] Staufen1 dsRDB4, 451 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
9	Staufen1 dsRDB4	[U-13C; U-15N]		451 uM
10	hARF1	Unlabelled		451 uM

26 2D 1H-13C HSQC

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 354 uM [U-15N] Staufen1 dsRBD4, 354 uM [U-13C] hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	Staufen1 dsRBD4	[U-15N]		354 uM
8	hARF1	[U-13C]		354 uM

27 2D 1H-1H TOCSY

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 310 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
11	hARF1	Unlabelled		310 uM

28 2D 1H-1H NOESY

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 310 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
11	hARF1	Unlabelled		310 uM

29 3D HCCH-COSY aromatic

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 478 uM [U-13C] hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
6	hARF1	[U-13C]		478 uM

30 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90-95% H2O/5-10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 417 uM [U-15N] Staufen1 dsRBD3, 417 uM Unlabelled hARF1, 90-95% H2O/5-10% D2O

#	Name	Isotope labeling	Type	Concentration
12	Staufen1 dsRBD3	[U-15N]		417 uM
13	hARF1	Unlabelled		417 uM

31 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
12	Staufen1 dsRBD3	[U-15N]		417 uM
13	hARF1	Unlabelled		417 uM

32 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
12	Staufen1 dsRBD3	[U-15N]		417 uM
13	hARF1	Unlabelled		417 uM

33 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE III - 700 MHz

Sample

State isotropic, Solvent system 90-95% H2O/5-10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 458 uM [U-13C; U-15N] Staufen1 dsRBD3, 458 uM Unlabelled hARF1, 90-95% H2O/5-10% D2O

#	Name	Isotope labeling	Type	Concentration
14	hARF1	Unlabelled		458 uM
15	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

34 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
14	hARF1	Unlabelled		458 uM
15	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

35 3D HNCA

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
14	hARF1	Unlabelled		458 uM
15	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

36 3D HNCO

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
14	hARF1	Unlabelled		458 uM
15	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

37 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
14	hARF1	Unlabelled		458 uM
15	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

38 3D HNCACB

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
14	hARF1	Unlabelled		458 uM
15	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

39 3D HBHA(CO)NH

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
14	hARF1	Unlabelled		458 uM
15	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

40 3D HA(CO)NH

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
14	hARF1	Unlabelled		458 uM
15	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

41 3D HcccoNH-TOCSY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
14	hARF1	Unlabelled		458 uM
15	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

42 3D CccoNH-TOCSY

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
14	hARF1	Unlabelled		458 uM
15	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

43 3D HCCH-TOCSY

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 458 uM [U-13C; U-15N] Staufen1 dsRBD3, 458 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
16	hARF1	Unlabelled		458 uM
17	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

44 3D HCCH-TOCSY

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 458 uM [U-13C; U-15N] Staufen1 dsRBD3, 458 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
16	hARF1	Unlabelled		458 uM
17	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

45 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
14	hARF1	Unlabelled		458 uM
15	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

46 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
14	hARF1	Unlabelled		458 uM
15	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

47 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 458 uM [U-13C; U-15N] Staufen1 dsRBD3, 458 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
16	hARF1	Unlabelled		458 uM
17	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

48 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 458 uM [U-13C; U-15N] Staufen1 dsRBD3, 458 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
16	hARF1	Unlabelled		458 uM
17	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

49 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
14	hARF1	Unlabelled		458 uM
15	Staufen1 dsRBD3	[U-13C; U-15N]		458 uM

50 3D 1H-15N NOESY

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
12	Staufen1 dsRBD3	[U-15N]		417 uM
13	hARF1	Unlabelled		417 uM

51 2D 1H-1H, 13C 2F-filtered NOESY

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 157 uM [U-13C; U-15N] Staufen1 dsRBD3, 157 uM Unlabelled Staufen1 dsRBD4, 157 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Concentration
18	Staufen1 dsRBD4	Unlabelled	157 uM
19	hARF1	Unlabelled	157 uM
20	Staufen1 dsRBD3	[U-13C; U-15N]	157 uM

52 2D 1H-1H, 13C 1F-filtered 2F-filtered NOESY

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
18	Staufen1 dsRBD4	Unlabelled	157 uM
19	hARF1	Unlabelled	157 uM
20	Staufen1 dsRBD3	[U-13C; U-15N]	157 uM

53 3D 13C 1F-filtered 2F-edited HSQC-NOESY

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Concentration
18	Staufen1 dsRBD4	Unlabelled	157 uM
19	hARF1	Unlabelled	157 uM
20	Staufen1 dsRBD3	[U-13C; U-15N]	157 uM

54 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	Staufen1 dsRBD4	[U-13C; U-15N]		405 uM
4	hARF1	Unlabelled		405 uM

55 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 850 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
3	Staufen1 dsRBD4	[U-13C; U-15N]		405 uM
4	hARF1	Unlabelled		405 uM

56 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 451 uM [U-13C; U-15N] Staufen1 dsRDB4, 451 uM Unlabelled hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
9	Staufen1 dsRDB4	[U-13C; U-15N]		451 uM
10	hARF1	Unlabelled		451 uM

57 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 478 uM [U-13C] hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
6	hARF1	[U-13C]		478 uM

58 2D 1H-13C HSQC ribose

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 354 uM [U-15N] Staufen1 dsRBD4, 354 uM [U-13C] hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	Staufen1 dsRBD4	[U-15N]		354 uM
8	hARF1	[U-13C]		354 uM

59 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5, Details 354 uM [U-15N] Staufen1 dsRBD4, 354 uM [U-13C] hARF1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	Staufen1 dsRBD4	[U-15N]		354 uM
8	hARF1	[U-13C]		354 uM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34421_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34421_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	2385		85.3 (chain: 1, length: 177), 100.0 (chain: 2, length: 34)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 177, Sequence coverage: 85.3%
  - Entity_assembly_ID: 2, Chain length: 34, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 1381
  - ¹³C chemical shifts: 848
  - ¹⁵N chemical shifts: 156
- Completeness of assignments
  - Entity_assemble_ID: 1
  - Entity_assemble_ID: 2
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords NMR structure of RNA-protein complex, RNA binding domain., RNA binding protein, Staufen mediated mRNA decay, Staufen1 protein, dsRBD-dsRNA binding protein

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1143	948	82.9
¹³C chemical shifts	821	662	80.6
¹⁵N chemical shifts	190	156	82.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	354	300	84.7
¹³C chemical shifts	354	283	79.9
¹⁵N chemical shifts	164	139	84.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	300	257	85.7
¹³C chemical shifts	227	186	81.9
¹⁵N chemical shifts	19	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	204	200	98.0
¹³C chemical shifts	170	168	98.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	96	57	59.4
¹³C chemical shifts	57	18	31.6
¹⁵N chemical shifts	19	0	0.0

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Found 1 Document (0.832 seconds)

BMRB: 34421

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Double-stranded RNA-binding protein Staufen homolog 1/RNA Complex, entity 1, : 1 : 2 : 122 entities Detail

Experiments performed 59 experiments Detail

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