BMRBj - BMREntryMaster

Found 1 Document (7.389 seconds)

BMRB: 34427

5YVG

Solution structure of the FUS/TLS RNA recognition motif in complex with U1 snRNA stem loop III

Authors

Campagne, S., Allain, F.H.

Assembly

RNA-binding protein FUS/RNA Complex

Entity

1. RNA-binding protein FUS/RNA Complex, entity 1 (polymer, Thiol state: not present), 131 monomers, 14117.27 Da Detail

GGFNKFGGPR DQGSRHDSEQ DNSDNNTIFV QGLGENVTIE SVADYFKQIG IIKTNKKTGQ PMINLYTDRE TGKLKGEATV SFDDPPSAKA AIDWFDGKEF SGNPIKVSFA TRRADFNRGG GNGRGGRGRG G

2. RNA-binding protein FUS/RNA Complex, entity 2 (polymer, Thiol state: not present), 28 monomers, 9017.343 Da Detail

GGGAUUUCCC CAAAUGUGGG AAACUCCC

Total weight

23134.613 Da

Max. entity weight

14117.27 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 86.7 %, Completeness (bb): 85.8 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	86.7 % (1665 of 1921)	86.9 % (882 of 1015)	86.7 % (643 of 742)	85.4 % (140 of 164)
Backbone	85.8 % (930 of 1084)	82.3 % (368 of 447)	86.1 % (440 of 511)	96.8 % (122 of 126)
Sidechain	89.1 % (843 of 946)	90.5 % (514 of 568)	91.5 % (311 of 340)	47.4 % (18 of 38)
Aromatic	76.8 % (189 of 246)	83.7 % (103 of 123)	78.0 % (85 of 109)	7.1 % (1 of 14)
Methyl	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (47 of 47)

1. entity 1

GGFNKFGGPR DQGSRHDSEQ DNSDNNTIFV QGLGENVTIE SVADYFKQIG IIKTNKKTGQ PMINLYTDRE TGKLKGEATV SFDDPPSAKA AIDWFDGKEF SGNPIKVSFA TRRADFNRGG GNGRGGRGRG G

2. entity 2

GGGAUUUCCC CAAAUGUGGG AAACUCCC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8 (±0.1), Details 1.2 mM [U-99% 13C; U-99% 15N] FUS/TLS RNA recognition motif (260-390), 1.5 mM SL3, 10 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
2	SL3	natural abundance	1.5 mM
3	sodium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	DTT	natural abundance	2 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8 (±0.1), Details 1.2 mM [U-99% 13C; U-99% 15N] FUS/TLS RNA recognition motif (260-390), 1.5 mM SL3, 10 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 100% D2O

#	Name	Isotope labeling	Concentration
6	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
7	SL3	natural abundance	1.5 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	50 mM
10	DTT	natural abundance	2 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8 (±0.1), Details 1 mM FUS/TLS RNA recognition motif (260-390), 0.8 mM [U-13C; U-15N] SL3, 10 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 100% D2O

#	Name	Isotope labeling	Concentration
11	FUS/TLS RNA recognition motif (260-390)	natural abundance	1 mM
12	SL3	[U-13C; U-15N]	0.8 mM
13	sodium phosphate	natural abundance	10 mM
14	sodium chloride	natural abundance	50 mM
15	DTT	natural abundance	2 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8 (±0.1), Details 1 mM FUS/TLS RNA recognition motif (260-390), 1 mM SL3, 10 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
16	FUS/TLS RNA recognition motif (260-390)	natural abundance	1 mM
17	SL3	natural abundance	1 mM
18	sodium phosphate	natural abundance	10 mM
19	sodium chloride	natural abundance	50 mM
20	DTT	natural abundance	2 mM

Release date

2019-10-23

Citation

Aberrant interaction of FUS with the U1 snRNA provides a molecular mechanism of FUS induced amyotrophic lateral sclerosis
Jutzi, D., Campagne, S., Schmidt, R., Reber, S., Mechtersheimer, J., Gypas, F., Schweingruber, C., Colombo, M., von Schroetter, C., Loughlin, F.E., Devoy, A., Hedlund, E., Zavolan, M., Allain, F.H., Ruepp, M.D.
Nat. Commun. (2020), 11, 6341-6341, PubMed 33311468 , DOI 10.1038/s41467-020-20191-3 , Abstract

Related entities 1. RNA-binding protein FUS/RNA Complex, entity 1, : 1 : 7 : 1 : 56 entities Detail

Related entities 2. RNA-binding protein FUS/RNA Complex, entity 2, : 2 : 4 entities Detail

Interaction partners 1. RNA-binding protein FUS/RNA Complex, entity 1, : 119 interactors Detail

More details for 119 interactors identified by 40 citations

Experiments performed 24 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 600 MHz cryo-probbed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8 (±0.1), Details 1.2 mM [U-99% 13C; U-99% 15N] FUS/TLS RNA recognition motif (260-390), 1.5 mM SL3, 10 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
2	SL3	natural abundance	1.5 mM
3	sodium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	DTT	natural abundance	2 mM

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE III - 600 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
1	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
2	SL3	natural abundance	1.5 mM
3	sodium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	DTT	natural abundance	2 mM

3 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 600 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
1	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
2	SL3	natural abundance	1.5 mM
3	sodium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	DTT	natural abundance	2 mM

4 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE III - 600 MHz cryo-probbed

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8 (±0.1), Details 1.2 mM [U-99% 13C; U-99% 15N] FUS/TLS RNA recognition motif (260-390), 1.5 mM SL3, 10 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 100% D2O

#	Name	Isotope labeling	Concentration
6	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
7	SL3	natural abundance	1.5 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	50 mM
10	DTT	natural abundance	2 mM

5 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 600 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
6	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
7	SL3	natural abundance	1.5 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	50 mM
10	DTT	natural abundance	2 mM

6 3D HCCH-TOCSY

Instrument

Bruker AVANCE III - 700 MHz cryo-probbed

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8 (±0.1), Details 1 mM FUS/TLS RNA recognition motif (260-390), 0.8 mM [U-13C; U-15N] SL3, 10 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 100% D2O

#	Name	Isotope labeling	Concentration
11	FUS/TLS RNA recognition motif (260-390)	natural abundance	1 mM
12	SL3	[U-13C; U-15N]	0.8 mM
13	sodium phosphate	natural abundance	10 mM
14	sodium chloride	natural abundance	50 mM
15	DTT	natural abundance	2 mM

7 3D 1H-13C NOESY

Instrument

Bruker AVANCE - 900 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
11	FUS/TLS RNA recognition motif (260-390)	natural abundance	1 mM
12	SL3	[U-13C; U-15N]	0.8 mM
13	sodium phosphate	natural abundance	10 mM
14	sodium chloride	natural abundance	50 mM
15	DTT	natural abundance	2 mM

8 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE - 900 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
11	FUS/TLS RNA recognition motif (260-390)	natural abundance	1 mM
12	SL3	[U-13C; U-15N]	0.8 mM
13	sodium phosphate	natural abundance	10 mM
14	sodium chloride	natural abundance	50 mM
15	DTT	natural abundance	2 mM

9 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE - 900 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
11	FUS/TLS RNA recognition motif (260-390)	natural abundance	1 mM
12	SL3	[U-13C; U-15N]	0.8 mM
13	sodium phosphate	natural abundance	10 mM
14	sodium chloride	natural abundance	50 mM
15	DTT	natural abundance	2 mM

10 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE III - 700 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
6	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
7	SL3	natural abundance	1.5 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	50 mM
10	DTT	natural abundance	2 mM

11 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE III - 700 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
6	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
7	SL3	natural abundance	1.5 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	50 mM
10	DTT	natural abundance	2 mM

12 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE - 900 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
1	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
2	SL3	natural abundance	1.5 mM
3	sodium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	DTT	natural abundance	2 mM

13 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE - 900 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
1	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
2	SL3	natural abundance	1.5 mM
3	sodium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	DTT	natural abundance	2 mM

14 3D 1H-15N NOESY

Instrument

Bruker AVANCE - 900 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
1	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
2	SL3	natural abundance	1.5 mM
3	sodium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	DTT	natural abundance	2 mM

15 2D 1H-1H NOESY

Instrument

Bruker AVANCE - 900 MHz cryo-probbed

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8 (±0.1), Details 1 mM FUS/TLS RNA recognition motif (260-390), 1 mM SL3, 10 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
16	FUS/TLS RNA recognition motif (260-390)	natural abundance	1 mM
17	SL3	natural abundance	1 mM
18	sodium phosphate	natural abundance	10 mM
19	sodium chloride	natural abundance	50 mM
20	DTT	natural abundance	2 mM

16 3D HNCA

Instrument

Bruker AVANCE III - 600 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
1	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
2	SL3	natural abundance	1.5 mM
3	sodium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	DTT	natural abundance	2 mM

17 3D HNCO

Instrument

Bruker AVANCE III - 600 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
1	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
2	SL3	natural abundance	1.5 mM
3	sodium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	DTT	natural abundance	2 mM

18 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 600 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
1	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
2	SL3	natural abundance	1.5 mM
3	sodium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	DTT	natural abundance	2 mM

19 3D HNCACB

Instrument

Bruker AVANCE III - 600 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
1	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
2	SL3	natural abundance	1.5 mM
3	sodium phosphate	natural abundance	10 mM
4	sodium chloride	natural abundance	50 mM
5	DTT	natural abundance	2 mM

20 2D F1fF2F TOCSY

Instrument

Bruker AVANCE III - 700 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
6	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
7	SL3	natural abundance	1.5 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	50 mM
10	DTT	natural abundance	2 mM

21 2D F2F NOEY

Instrument

Bruker AVANCE III - 700 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
6	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
7	SL3	natural abundance	1.5 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	50 mM
10	DTT	natural abundance	2 mM

22 2D F1FF2f NOESY

Instrument

Bruker AVANCE III - 700 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
6	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
7	SL3	natural abundance	1.5 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	50 mM
10	DTT	natural abundance	2 mM

23 3D 1H-13C filtered edited NOESY HSQC

Instrument

Bruker AVANCE - 900 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
11	FUS/TLS RNA recognition motif (260-390)	natural abundance	1 mM
12	SL3	[U-13C; U-15N]	0.8 mM
13	sodium phosphate	natural abundance	10 mM
14	sodium chloride	natural abundance	50 mM
15	DTT	natural abundance	2 mM

24 3D 1H-13C filtered edited NOESY HSQC

Instrument

Bruker AVANCE - 900 MHz cryo-probbed

Sample

#	Name	Isotope labeling	Concentration
6	FUS/TLS RNA recognition motif (260-390)	[U-99% 13C; U-99% 15N]	1.2 mM
7	SL3	natural abundance	1.5 mM
8	sodium phosphate	natural abundance	10 mM
9	sodium chloride	natural abundance	50 mM
10	DTT	natural abundance	2 mM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34427_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34427_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1761		96.9 (chain: 1, length: 131), 100.0 (chain: 2, length: 28)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 131, Sequence coverage: 96.9%
  - Entity_assembly_ID: 2, Chain length: 28, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 981
  - ¹³C chemical shifts: 641
  - ¹⁵N chemical shifts: 139
- Completeness of assignments
  - Entity_assemble_ID: 1
  - Entity_assemble_ID: 2
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	769	734	95.4
¹³C chemical shifts	553	506	91.5
¹⁵N chemical shifts	151	139	92.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	279	264	94.6
¹³C chemical shifts	262	243	92.7
¹⁵N chemical shifts	126	121	96.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	490	470	95.9
¹³C chemical shifts	291	263	90.4
¹⁵N chemical shifts	25	18	72.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	48	48	100.0
¹³C chemical shifts	48	48	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	61	55	90.2
¹³C chemical shifts	60	37	61.7
¹⁵N chemical shifts	1	1	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	246	151	61.4
¹³C chemical shifts	189	135	71.4
¹⁵N chemical shifts	13	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	168	103	61.3
¹³C chemical shifts	140	87	62.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	78	48	61.5
¹³C chemical shifts	49	48	98.0
¹⁵N chemical shifts	13	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	21	21	100.0
¹³C chemical shifts	21	21	100.0

Keywords FUS-U1snRNP, SPLICING, Spliceosome, Splicing regulation

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Found 1 Document (7.389 seconds)

BMRB: 34427

Sample #1

Sample #2

Sample #3

Sample #4

Related entities 1. RNA-binding protein FUS/RNA Complex, entity 1, : 1 : 7 : 1 : 56 entities Detail

Related entities 2. RNA-binding protein FUS/RNA Complex, entity 2, : 2 : 4 entities Detail

Interaction partners 1. RNA-binding protein FUS/RNA Complex, entity 1, : 119 interactors Detail

Experiments performed 24 experiments Detail

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