BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	98.2 % (1301 of 1325)	97.4 % (678 of 696)	99.4 % (503 of 506)	97.6 % (120 of 123)
Backbone	98.0 % (651 of 664)	96.9 % (221 of 228)	99.1 % (325 of 328)	97.2 % (105 of 108)
Sidechain	98.6 % (754 of 765)	97.6 % (457 of 468)	100.0 % (282 of 282)	100.0 % (15 of 15)
Aromatic	100.0 % (68 of 68)	100.0 % (34 of 34)	100.0 % (34 of 34)
Methyl	100.0 % (128 of 128)	100.0 % (64 of 64)	100.0 % (64 of 64)

1. entity 1

GAMAQRKGAG RVVHICNLPE GSCTENDVIN LGLPFGKVTN YILMKSTNQA FLEMAYTEAA QAMVQYYQEK PAIINGEKLL IRMSTRYKEL QLKKPGKNVA AIIQDIHSQR ER

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 400 uM [U-99% 13C; U-99% 15N] Mouse RBM20 RRM domain, 20 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 500 uM Mouse RBM20 RRM domain, 20 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	Mouse RBM20 RRM domain	natural abundance	500 uM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	50 mM
8	DTT	natural abundance	2 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 170 uM [U-99% 13C] Mouse RBM20 RRM domain, 20 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 100% D2O

#	Name	Isotope labeling	Concentration
9	Mouse RBM20 RRM domain	[U-99% 13C]	170 uM
10	sodium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	50 mM
12	DTT	natural abundance	2 mM

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 100 uM [U-10% 13C; U-99% 15N] Mouse RBM20 RRM domain, 20 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
13	Mouse RBM20 RRM domain	[U-10% 13C; U-99% 15N]	100 uM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	50 mM
16	DTT	natural abundance	2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.39 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 6SOE, Strand ID: A Detail

Release date

2019-10-24

Citation

Structural basis of UCUU RNA motif recognition by splicing factor RBM20
Upadhyay, S.K., Mackereth, C.D.
Nucleic Acids Res. (2020), 48, 4538-4550, PubMed 32187365 , DOI 10.1093/nar/gkaa168 , Abstract

Related entities 1. RNA-binding protein 20, : 1 : 1 : 2 : 41 entities Detail

Interaction partners 1. RNA-binding protein 20, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 800 MHz cryoprobe TCI

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 400 uM [U-99% 13C; U-99% 15N] Mouse RBM20 RRM domain, 20 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

2 3D HNCO

Instrument

Bruker AVANCE III - 800 MHz cryoprobe TCI

Sample

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

3 3D HN(CA)CO

Instrument

Bruker AVANCE III - 800 MHz cryoprobe TCI

Sample

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

4 3D HNCA

Instrument

Bruker AVANCE III - 800 MHz cryoprobe TCI

Sample

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

5 3D HNCACB

Instrument

Bruker AVANCE III - 800 MHz cryoprobe TCI

Sample

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

6 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 800 MHz cryoprobe TCI

Sample

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

7 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE III - 800 MHz cryoprobe TCI

Sample

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

8 3D H(C)(CO)NH-TOCSY

Instrument

Bruker AVANCE III - 800 MHz cryoprobe TCI

Sample

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

9 3D (H)C(CO)NH-TOCSY

Instrument

Bruker AVANCE III - 800 MHz cryoprobe TCI

Sample

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

10 3D HCCH-TOCSY

Instrument

Bruker AVANCE III - 800 MHz cryoprobe TCI

Sample

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

11 3D HCCH-TOCSY

Instrument

Bruker AVANCE NEO - 700 MHz TXI

Sample

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

12 2D 1H-13C HSQC CT

Instrument

Bruker AVANCE NEO - 700 MHz TXI

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 100 uM [U-10% 13C; U-99% 15N] Mouse RBM20 RRM domain, 20 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
13	Mouse RBM20 RRM domain	[U-10% 13C; U-99% 15N]	100 uM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	50 mM
16	DTT	natural abundance	2 mM

13 3D 1H-15N NOESY

Instrument

Bruker AVANCE III - 800 MHz cryoprobe TCI

Sample

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

14 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE III - 800 MHz cryoprobe TCI

Sample

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

15 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE NEO - 700 MHz TXI

Sample

#	Name	Isotope labeling	Concentration
1	Mouse RBM20 RRM domain	[U-99% 13C; U-99% 15N]	400 uM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	2 mM

16 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 800 MHz cryoprobe TCI

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 170 uM [U-99% 13C] Mouse RBM20 RRM domain, 20 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 100% D2O

#	Name	Isotope labeling	Concentration
9	Mouse RBM20 RRM domain	[U-99% 13C]	170 uM
10	sodium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	50 mM
12	DTT	natural abundance	2 mM

17 2D 1H-1H NOESY

Instrument

Bruker AVANCE NEO - 700 MHz TXI

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 500 uM Mouse RBM20 RRM domain, 20 mM sodium phosphate, 50 mM sodium chloride, 2 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	Mouse RBM20 RRM domain	natural abundance	500 uM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	50 mM
8	DTT	natural abundance	2 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34429_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34429_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1458		99.1 (chain: 1, length: 112)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1

Status: Warning

Sequence coverage of experimental data

Entity_assembly_ID: 1, Chain length: 112, Sequence coverage: 99.1%

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
GAMAQRKGAGRVVHICNLPEGSCTENDVINLGLPFGKVTNYILMKSTNQAFLEMAYTEAAQAMVQYYQEKPAIINGEKLLIRMSTRYKELQLKKPGKNVA
 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
.AMAQRKGAGRVVHICNLPEGSCTENDVINLGLPFGKVTNYILMKSTNQAFLEMAYTEAAQAMVQYYQEKPAIINGEKLLIRMSTRYKELQLKKPGKNVA

-------110--
AIIQDIHSQRER
||||||||||||
AIIQDIHSQRER

Total number of assignments
- ¹H chemical shifts: 821
- ¹³C chemical shifts: 518
- ¹⁵N chemical shifts: 119

Completeness of assignments

Entity_assemble_ID: 1

Excluded Atom_ID in statistics

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
5	GLN	CD	180.4
17	ASN	CG	178.623
26	ASN	CG	176.093
30	ASN	CG	176.253
40	ASN	CG	176.839
48	ASN	CG	178.5
49	GLN	CD	181.312
61	GLN	CD	179.957
65	GLN	CD	179.986
68	GLN	CD	179.876
75	ASN	CG	177.769
91	GLN	CD	180.366
98	ASN	CG	177.06
104	GLN	CD	180.4
109	GLN	CD	180.4

All atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	696	683	98.1
¹³C chemical shifts	506	503	99.4
¹⁵N chemical shifts	129	119	92.2

Backbone atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	228	221	96.9
¹³C chemical shifts	224	221	98.7
¹⁵N chemical shifts	108	104	96.3

Side chain atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	468	462	98.7
¹³C chemical shifts	282	282	100.0
¹⁵N chemical shifts	21	15	71.4

Methyl group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	69	69	100.0
¹³C chemical shifts	69	69	100.0

Aromatic group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	34	34	100.0
¹³C chemical shifts	34	34	100.0

Redox state of CYS
Peptide bond of PRO
Tautomeric state of HIS
Rotameric state of ILE , LEU, and VAL
Histogram of normalized assigned chemical shifts
Random coil index and derived parameters (S² and NMR RMSD)
- Chain_ID: 1

Keywords RNA BINDING PROTEIN, alternative splicing

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Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.92 seconds)

BMRB: 34429

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. RNA-binding protein 20, : 1 : 1 : 2 : 41 entities Detail

Interaction partners 1. RNA-binding protein 20, : 2 interactors Detail

Experiments performed 17 experiments Detail

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Chemical shift validation 3 contents Detail