BMRB: 34437

Structure of the BRK domain of the SWI/SNF chromatin remodelling complex subunit BRG1 reveals a potential role in protein-protein interactions

Authors

Allen, M.D., Bycoft, M., Zinzalla, G.

Assembly

Transcription activator BRG1

Entity

1. Transcription activator BRG1 (polymer, Thiol state: not present), 49 monomers, 5282.864 Da Detail

SQMSDLPVKV IHVESGKILT GTDAPKAGQL EAWLEMNPGY EVAPRSDSE

Formula weight

5282.864 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.0 %, Completeness (bb): 98.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	93.0 % (506 of 544)	99.6 % (282 of 283)	83.5 % (177 of 212)	95.9 % (47 of 49)
Backbone	98.6 % (282 of 286)	99.0 % (97 of 98)	98.6 % (141 of 143)	97.8 % (44 of 45)
Sidechain	88.8 % (269 of 303)	100.0 % (185 of 185)	71.1 % (81 of 114)	75.0 % (3 of 4)
Aromatic	50.0 % (12 of 24)	100.0 % (12 of 12)	0.0 % (0 of 11)	0.0 % (0 of 1)
Methyl	100.0 % (52 of 52)	100.0 % (26 of 26)	100.0 % (26 of 26)

1. entity 1

SQMSDLPVKV IHVESGKILT GTDAPKAGQL EAWLEMNPGY EVAPRSDSE

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Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 293 (±0.1) K, pH 6.5 (±0.05), Details 1 mM [U-99% 13C; U-99% 15N] BRG1 BRK domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	BRG1 BRK domain	[U-99% 13C; U-99% 15N]		1 mM
2	potassium phosphate	natural abundance		20 mM
3	NaCl	natural abundance		100 mM
4	beta-mercaptoethanol	natural abundance		5 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 293 (±0.1) K, pH 6.5 (±0.05), Details 2 mM unlabelled BRG1 BRK domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
5	BRG1 BRK domain	natural abundance		2 mM
6	potassium phosphate	natural abundance		20 mM
7	NaCl	natural abundance		100 mM
8	beta-mercaptoethanol	natural abundance		5 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 293 (±0.1) K, pH 6.5 (±0.05), Details 1 mM [U-10% 13C] BRG1 BRK domain, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
9	BRG1 BRK domain	[U-10% 13C]		1 mM
10	potassium phosphate	natural abundance		20 mM
11	NaCl	natural abundance		100 mM
12	beta-mercaptoethanol	natural abundance		5 mM

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LACS Plot; CA

Referencing offset: -0.8 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl carbons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	indirect	1.0
15N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.8 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl carbons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	indirect	1.0
15N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.06 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl carbons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	indirect	1.0
15N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.38 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl carbons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	indirect	1.0
15N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 6SY2, Strand ID: A Detail

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Release date

2019-11-05

Citation

Structure of the BRK domain of the SWI/SNF chromatin remodeling complex subunit BRG1 reveals a potential role in protein-protein interactions
Allen, M.D., Bycoft, M., Zinzalla, G.
Protein Sci. (2020), 29, 1047-1053, PubMed 31909846 , DOI 10.1002/pro.3820 , Abstract

Related entities 1. Transcription activator BRG1, : 1 : 7 : 16 entities Detail

Interaction partners 1. Transcription activator BRG1, : 105 interactors Detail

Experiments performed 11 experiments Detail

Chemical shift validation 3 contents Detail