BMRBj - BMREntryMaster

Found 1 Document (0.801 seconds)

BMRB: 34443

6TAZ

Timeless couples G quadruplex detection with processing by DDX11 during DNA replication

Authors

Lerner Koch, L., Holzer, S., Kilkenny, M.L., Murat, P., Svikovic, S., Schiavone, D., Bittleston, A., Maman, J.D., Branzei, D., Stott, K., Pellegrini, L., Sale, E.J.

Assembly

Protein timeless homolog

Entity

1. Protein timeless homolog (polymer, Thiol state: not present), 141 monomers, 16328.51 Da Detail

DPSRRAPTWS PEEEAHLREL YLANKDVEGQ DVVEAILAHL NTVPRTRKQI IHHLVQMGLA DSVKDFQRKG THIVLWTGDQ ELELQRLFEE FRDSDDVLGH IMKNITAKRS RARIVDKLLA LGLVAERREL YKKRQKKLAS S

Formula weight

16328.51 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.1 %, Completeness (bb): 98.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.1 % (1645 of 1695)	96.2 % (857 of 891)	97.7 % (640 of 655)	99.3 % (148 of 149)
Backbone	98.3 % (824 of 838)	97.9 % (278 of 284)	98.1 % (409 of 417)	100.0 % (137 of 137)
Sidechain	96.3 % (955 of 992)	95.4 % (579 of 607)	97.9 % (365 of 373)	91.7 % (11 of 12)
Aromatic	100.0 % (94 of 94)	100.0 % (47 of 47)	100.0 % (45 of 45)	100.0 % (2 of 2)
Methyl	100.0 % (174 of 174)	100.0 % (87 of 87)	100.0 % (87 of 87)

1. entity 1

DPSRRAPTWS PEEEAHLREL YLANKDVEGQ DVVEAILAHL NTVPRTRKQI IHHLVQMGLA DSVKDFQRKG THIVLWTGDQ ELELQRLFEE FRDSDDVLGH IMKNITAKRS RARIVDKLLA LGLVAERREL YKKRQKKLAS S

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4, Details 0.5 mM [U-13C; U-15N] TimDBD, 10 mM NA sodium phosphate, 40 mM NA potassium chloride, 0.5 mM NA EDTA, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	TimDBD	[U-13C; U-15N]	0.5 mM
2	sodium phosphate	NA	10 mM
3	potassium chloride	NA	40 mM
4	EDTA	NA	0.5 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 6TAZ, Strand ID: B Detail

Release date

2020-06-24

Citation

Timeless couples G-quadruplex detection with processing by DDX11 helicase during DNA replication
Lerner, L.K., Holzer, S., Kilkenny, M.L., Svikovic, S., Murat, P., Schiavone, D., Eldridge, C.B., Bittleston, A., Maman, J.D., Branzei, D., Stott, K., Pellegrini, L., Sale, J.E.
EMBO J. (2020), e104185-e104185, PubMed 32705708 , DOI 10.15252/embj.2019104185 , Abstract

Related entities 1. Protein timeless homolog, : 1 : 2 : 9 entities Detail

Interaction partners 1. Protein timeless homolog, : 13 interactors Detail

More details for 13 interactors identified by 5 citations

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34443_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34443_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1830		99.3 (chain: 1, length: 141)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 141, Sequence coverage: 99.3%
- Total number of assignments
  - ¹H chemical shifts: 1041
  - ¹³C chemical shifts: 638
  - ¹⁵N chemical shifts: 151
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	891	863	96.9
¹³C chemical shifts	655	638	97.4
¹⁵N chemical shifts	163	151	92.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	284	282	99.3
¹³C chemical shifts	282	274	97.2
¹⁵N chemical shifts	137	136	99.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	607	581	95.7
¹³C chemical shifts	373	364	97.6
¹⁵N chemical shifts	26	15	57.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	89	89	100.0
¹³C chemical shifts	89	89	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	47	47	100.0
¹³C chemical shifts	45	45	100.0
¹⁵N chemical shifts	2	2	100.0

Keywords 3-helix bundle, DNA BINDING PROTEIN, DNA-binding protein, tandem repeat

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Found 1 Document (0.801 seconds)

BMRB: 34443

Sample

Related entities 1. Protein timeless homolog, : 1 : 2 : 9 entities Detail

Interaction partners 1. Protein timeless homolog, : 13 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail