BMRB: 34444

2'-F-arabinoguanosine and 2'-F-riboguanosine modified hybrid type G-quadruplex with V-loop

Authors

Haase, L., Weisz, K.

Assembly

F1415

Entity

1. F1415 (polymer, Thiol state: not present), 22 monomers, 7071.443 Da Detail

GGGATGGGAC ACAXXGGACG GG

Formula weight

7071.443 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 81.8 %, Completeness: 54.5 %, Completeness (bb): 49.3 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polydeoxyribonucleotide

	Total	1H
All	54.5 % (102 of 187)	54.5 % (102 of 187)
Suger, PO4	49.3 % (69 of 140)	49.3 % (69 of 140)
Nucleobase	70.2 % (33 of 47)	70.2 % (33 of 47)
Aromatic	80.0 % (32 of 40)	80.0 % (32 of 40)
Methyl	100.0 % (1 of 1)	100.0 % (1 of 1)

1. entity 1

GGGATGGGAC ACAXXGGACG GG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM A14F15, 90% H2O/10% D2O. 10 mM potassium phosphate buffer at pH 7, i.e. 6.13 mM KH2PO4 and 3.87 mM K2HPO4.

#	Name	Isotope labeling	Type	Concentration
1	A14F15	natural abundance		0.4 mM
2	potassium phosphate buffer	natural abundance		10 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.4 mM A14F15, 100% D2O. 10 mM potassium phosphate buffer at pH 7, i.e. 6.13 mM KH2PO4 and 3.87 mM K2HPO4.

#	Name	Isotope labeling	Type	Concentration
3	A14F15	natural abundance		0.4 mM
4	potassium phosphate buffer	natural abundance		10 mM

Hide sample codition

Release date

2020-03-18

Citation

Switching the type of V-loop in sugar-modified G-quadruplexes through altered fluorine interactions
Haase, L., Weisz, K.
Chem. Commun. (Camb). (2020), 56, 4539-4542, PubMed 32202271 , DOI 10.1039/d0cc01285h , Abstract

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail