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BMRB: 34447

6TDM

Bam_5920cDD 5919nDD docking domains

Authors

Risser, F., Chagot, B.

Assembly

Beta-ketoacyl synthase

Entity

1. Beta-ketoacyl synthase (polymer, Thiol state: not present), 64 monomers, 6727.423 × 2 Da Detail

GPGSYDAALP IDELSALLRQ EMGDDGGGSG GGSMQDIQQL LAKSLTEIKR LKAANQALEQ ARRE

Total weight

13454.846 Da

Max. entity weight

6727.423 Da

Source organism

Burkholderia ambifaria AMMD

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.6 %, Completeness (bb): 97.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.6 % (688 of 698)	99.7 % (364 of 365)	96.9 % (252 of 260)	98.6 % (72 of 73)
Backbone	97.9 % (372 of 380)	99.3 % (134 of 135)	96.7 % (177 of 183)	98.4 % (61 of 62)
Sidechain	99.5 % (371 of 373)	100.0 % (230 of 230)	98.5 % (130 of 132)	100.0 % (11 of 11)
Aromatic	75.0 % (6 of 8)	100.0 % (4 of 4)	50.0 % (2 of 4)
Methyl	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)

1. entity 1

GPGSYDAALP IDELSALLRQ EMGDDGGGSG GGSMQDIQQL LAKSLTEIKR LKAANQALEQ ARRE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-100% 13C; U-100% 15N]		1 mM

Release date

2020-07-13

Citation

Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: Docking in the enacyloxin IIa polyketide synthase
Risser, F., Collin, S., Dos Santos-Morais, R., Gruez, A., Chagot, B., Weissman, K.J.
J. Struct. Biol. (2020), 212, 107581-107581, PubMed 32717326 , DOI 10.1016/j.jsb.2020.107581 , Abstract

Related entities 1. Beta-ketoacyl synthase, : 1 : 2 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-100% 13C; U-100% 15N]		1 mM

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-100% 13C; U-100% 15N]		1 mM

3 3D HNCO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-100% 13C; U-100% 15N]		1 mM

4 3D CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-100% 13C; U-100% 15N]		1 mM

5 3D 1H-13C NOESY aromatic

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-100% 13C; U-100% 15N]		1 mM

6 3D 1H-13C NOESY aliphatic

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-100% 13C; U-100% 15N]		1 mM

7 3D 1H-15N NOESY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-100% 13C; U-100% 15N]		1 mM

8 3D C(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-100% 13C; U-100% 15N]		1 mM

9 3D H(CCO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-100% 13C; U-100% 15N]		1 mM

10 3D HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-100% 13C; U-100% 15N]		1 mM

11 3D HNCACB

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-100% 13C; U-100% 15N]		1 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34447_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34447_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	1516		100.0 (chain: 1, length: 64)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 64, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 868
  - ¹³C chemical shifts: 504
  - ¹⁵N chemical shifts: 144
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords PROTEIN BINDING, docking domain, polyketide synthase