BMRB: 34465

Structure of a protein-RNA complex by ssNMR

Authors

Mumdooh, A., Marchanka, A., Carlomagno, T.

Assembly

50S ribosomal protein L7Ae/RNA Complex

Entity

1. 50S ribosomal protein L7Ae/RNA Complex, entity 1 (polymer, Thiol state: not reported), 123 monomers, 13394.50 Da Detail

MAKPSYVKFE VPKELAEKAL QAVEIARDTG KIRKGTNETT KAVERGQAKL VIIAEDVDPE EIVAHLPPLC EEKEIPYIYV PSKKELGAAA GIEVAAASVA IIEPGKARDL VEEIAMKVKE LMK

2. 50S ribosomal protein L7Ae/RNA Complex, entity 2 (polymer, Thiol state: not present), 26 monomers, 8470.044 Da Detail

GCUGAGCUCG AAAGAGCAAU GAUGUC

Total weight

21864.543 Da

Max. entity weight

13394.5 Da

Source organism

Pyrococcus furiosus

Exptl. method

NMR

Refine. method

na

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 67.1 %, Completeness: 50.4 %, Completeness (bb): 59.9 % Detail

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Polymer type: polypeptide(L) polyribonucleotide

	Total	13C	15N
All	50.4 % (437 of 867)	46.6 % (343 of 736)	71.8 % (94 of 131)
Backbone	59.9 % (364 of 608)	54.8 % (270 of 493)	81.7 % (94 of 115)
Sidechain	39.6 % (149 of 376)	41.4 % (149 of 360)	0.0 % (0 of 16)
Aromatic	0.0 % (0 of 76)	0.0 % (0 of 63)	0.0 % (0 of 13)
Methyl	76.2 % (64 of 84)	76.2 % (64 of 84)

1. entity 1

MAKPSYVKFE VPKELAEKAL QAVEIARDTG KIRKGTNETT KAVERGQAKL VIIAEDVDPE EIVAHLPPLC EEKEIPYIYV PSKKELGAAA GIEVAAASVA IIEPGKARDL VEEIAMKVKE LMK

2. entity 2

GCUGAGCUCG AAAGAGCAAU GAUGUC

Show sample condition

Sample #1

Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 4 mg/mL [U-99% 13C; U-99% 15N] 50S RIBOSOMAL PROTEIN L7AE, solid

#	Name	Isotope labeling	Type	Concentration
1	50S RIBOSOMAL PROTEIN L7AE (U-13C, U-15N)	[U-99% 13C; U-99% 15N]		4 mg/mL
2	HEPES	natural abundance		25 mM
3	NaCl	natural abundance		120 mM

Sample #2

Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 8 mg/mL [U-99% 13C; U-99% 15N] 50S RIBOSOMAL PROTEIN L7AE, 5 mg/mL RNA (26-MER), solid

#	Name	Isotope labeling	Type	Concentration
4	50S RIBOSOMAL PROTEIN L7AE (U-13C, U-15N)	[U-99% 13C; U-99% 15N]		8 mg/mL
5	RNA (26-MER)	natural abundance		5 mg/mL
6	HEPES	natural abundance		25 mM
7	NaCl	natural abundance		120 mM

Sample #3

Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 4 mg/mL [U-99% 13C; U-99% 15N] 50S RIBOSOMAL PROTEIN L7AE, 12 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 10 mg/mL RNA (26-MER), solid

#	Name	Isotope labeling	Type	Concentration
8	50S RIBOSOMAL PROTEIN L7AE (U-13C, U-15N)	[U-99% 13C; U-99% 15N]		4 mg/mL
9	RNA (26-MER)	natural abundance		10 mg/mL
10	50S RIBOSOMAL PROTEIN L7AE	natural abundance		12 mg/mL
11	HEPES	natural abundance		25 mM
12	NaCl	natural abundance		120 mM

Sample #4

Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 16 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 2 mg/mL [U-13C; U-15N]-Ade RNA (26-MER), 8 mg/mL RNA (26-MER), solid

#	Name	Isotope labeling	Type	Concentration
13	RNA (26-MER)	natural abundance		8 mg/mL
14	50S RIBOSOMAL PROTEIN L7AE	natural abundance		16 mg/mL
15	RNA (26-MER) (U-13C, U-15N-ade)	[U-13C; U-15N]-Ade		2 mg/mL
16	HEPES	natural abundance		25 mM
17	NaCl	natural abundance		120 mM

Sample #5

Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 16 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 2 mg/mL [U-13C; U-15N]-Ura RNA (26-MER), 8 mg/mL RNA (26-MER), solid

#	Name	Isotope labeling	Type	Concentration
18	RNA (26-MER)	natural abundance		8 mg/mL
19	50S RIBOSOMAL PROTEIN L7AE	natural abundance		16 mg/mL
20	RNA (26-MER) (U-13C, U-15N-ura)	[U-13C; U-15N]-Ura		2 mg/mL
21	HEPES	natural abundance		25 mM
22	NaCl	natural abundance		120 mM

Sample #6

Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 4 mg/mL [U-99% 13C; U-99% 15N] 50S RIBOSOMAL PROTEIN L7AE, 12 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 10 mg/mL RNA (26-MER), solid

#	Name	Isotope labeling	Type	Concentration
23	50S RIBOSOMAL PROTEIN L7AE (U-13C, U-15N)	[U-99% 13C; U-99% 15N]		4 mg/mL
24	RNA (26-MER)	natural abundance		10 mg/mL
25	50S RIBOSOMAL PROTEIN L7AE	natural abundance		12 mg/mL
26	HEPES	natural abundance		25 mM
27	NaCl	natural abundance		120 mM

Sample #7

Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 16 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 2 mg/mL [U-13C; U-15N]-Ade RNA (26-MER), 8 mg/mL RNA (26-MER), solid

#	Name	Isotope labeling	Type	Concentration
28	RNA (26-MER)	natural abundance		8 mg/mL
29	50S RIBOSOMAL PROTEIN L7AE	natural abundance		16 mg/mL
30	RNA (26-MER) (U-13C, U-15N-ade)	[U-13C; U-15N]-Ade		2 mg/mL
31	HEPES	natural abundance		25 mM
32	NaCl	natural abundance		120 mM

Sample #8

Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 16 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 2 mg/mL [U-13C; U-15N]-Ura RNA (26-MER), 8 mg/mL RNA (26-MER), solid

#	Name	Isotope labeling	Type	Concentration
33	RNA (26-MER)	natural abundance		8 mg/mL
34	50S RIBOSOMAL PROTEIN L7AE	natural abundance		16 mg/mL
35	RNA (26-MER) (U-13C, U-15N-ura)	[U-13C; U-15N]-Ura		2 mg/mL
36	HEPES	natural abundance		25 mM
37	NaCl	natural abundance		120 mM

Sample #9

Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 4 mg/mL [U-99% 13C; U-99% 15N] 50S RIBOSOMAL PROTEIN L7AE, 12 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 10 mg/mL RNA (26-MER), solid

#	Name	Isotope labeling	Type	Concentration
38	50S RIBOSOMAL PROTEIN L7AE (U-13C, U-15N)	[U-99% 13C; U-99% 15N]		4 mg/mL
39	RNA (26-MER)	natural abundance		10 mg/mL
40	50S RIBOSOMAL PROTEIN L7AE	natural abundance		12 mg/mL
41	HEPES	natural abundance		25 mM
42	NaCl	natural abundance		120 mM

Sample #10

Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 16 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 2 mg/mL [U-13C; U-15N]-Ade RNA (26-MER), 8 mg/mL RNA (26-MER), solid

#	Name	Isotope labeling	Type	Concentration
43	RNA (26-MER)	natural abundance		8 mg/mL
44	50S RIBOSOMAL PROTEIN L7AE	natural abundance		16 mg/mL
45	RNA (26-MER) (U-13C, U-15N-ade)	[U-13C; U-15N]-Ade		2 mg/mL
46	HEPES	natural abundance		25 mM
47	NaCl	natural abundance		120 mM

Sample #11

Solvent system solid, Pressure 1 bar, Temperature 265 K, pH 7.6, Details 16 mg/mL 50S RIBOSOMAL PROTEIN L7AE, 2 mg/mL [U-13C; U-15N]-Ura RNA (26-MER), 8 mg/L RNA (26-MER), solid

#	Name	Isotope labeling	Type	Concentration
48	RNA (26-MER)	natural abundance		8 mg/mL
49	50S RIBOSOMAL PROTEIN L7AE	natural abundance		16 mg/mL
50	RNA (26-MER) (U-13C, U-15N-ura)	[U-13C; U-15N]-Ura		2 mg/mL
51	HEPES	natural abundance		25 mM
52	NaCl	natural abundance		120 mM

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Calculated from 10 models in PDB: 6TPH, Strand ID: A Detail

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Release date

2020-01-29

Citation

Structure of a protein-RNA complex by ssNMR
Ahmed, M., Marchanka, A., Carlomagno, T.
Angew. Chem Int Ed Engl. (2020), 59, 6866-6873, PubMed 32023357 , DOI 10.1002/anie.201915465 , Abstract

Related entities 1. 50S ribosomal protein L7Ae/RNA Complex, entity 1, : 1 : 12 : 13 : 8 : 160 entities Detail

Related entities 2. 50S ribosomal protein L7Ae/RNA Complex, entity 2, : 1 : 1 entities Detail

Interaction partners 1. 50S ribosomal protein L7Ae/RNA Complex, entity 1, : 4 interactors Detail

Experiments performed 17 experiments Detail

Chemical shift validation 4 contents Detail