BMRBj - BMREntryMaster

Found 1 Document (0.639 seconds)

BMRB: 34492

drosophila Unr CSD78

Authors

Hollmann, N.M., Simon, B., Hennig, J.

Assembly

Upstream of N-ras, isoform A

Entity

1. Upstream of N-ras, isoform A (polymer), 168 monomers, 18653.77 Da Detail

GAGSDAGQVY RGFIAVMKEN FGFIETLSHD EEVFFHFSNY MGNPNWLELG QEVEYTLARN GNTSVSGNCL PAENVRMLPK NSIPQPAVLE TTHNGVVARP LRCINPDQQE YAGLIEILDE LRTTVISQHE FGITSLVNKR DLLQKGDLVS FRIDESGRAA CVNAVRQK

Formula weight

18653.77 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 93.6 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.6 % (1803 of 1926)	96.1 % (960 of 999)	90.2 % (671 of 744)	94.0 % (172 of 183)
Backbone	95.6 % (950 of 994)	97.4 % (334 of 343)	93.9 % (460 of 490)	96.9 % (156 of 161)
Sidechain	92.6 % (1006 of 1086)	95.4 % (626 of 656)	89.2 % (364 of 408)	72.7 % (16 of 22)
Aromatic	73.6 % (103 of 140)	95.7 % (67 of 70)	50.7 % (35 of 69)	100.0 % (1 of 1)
Methyl	95.3 % (181 of 190)	96.8 % (92 of 95)	93.7 % (89 of 95)

1. entity 1

GAGSDAGQVY RGFIAVMKEN FGFIETLSHD EEVFFHFSNY MGNPNWLELG QEVEYTLARN GNTSVSGNCL PAENVRMLPK NSIPQPAVLE TTHNGVVARP LRCINPDQQE YAGLIEILDE LRTTVISQHE FGITSLVNKR DLLQKGDLVS FRIDESGRAA CVNAVRQK

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4, Details 0.5 mM [U-99% 13C; U-99% 15N] dCSD78, 150 mM sodium chloride, 20 mM sodium phosphate, 1 mM DTT, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	dCSD78	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4, Details 0.5 mM [U-99% 13C; U-99% 15N] dCSD78, 150 mM sodium chloride, 20 mM sodium phosphate, 1 mM DTT, 100% D2O

#	Name	Isotope labeling	Concentration
5	dCSD78	[U-99% 13C; U-99% 15N]	0.5 mM
6	sodium chloride	natural abundance	150 mM
7	sodium phosphate	natural abundance	20 mM
8	DTT	natural abundance	1 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 6Y4H, Strand ID: A Detail

Release date

2020-02-27

Citation

Pseudo-RNA-Binding Domains Mediate RNA Structure Specificity in Upstream of N-Ras
Hollmann, N.M., Jagtap, P.K.A., Masiewicz, P., Guitart, T., Simon, B., Provaznik, J., Stein, F., Haberkant, P., Sweetapple, L.J., Villacorte, L., Mooijman, D., Benes, V., Savitski, M., Gebauer, F., Hennig, J.
Cell Rep. (2020), 32, 107930-107930, PubMed 32697992 , DOI 10.1016/j.celrep.2020.107930 , Abstract

Related entities 1. Upstream of N-ras, isoform A, : 1 : 12 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4, Details 0.5 mM [U-99% 13C; U-99% 15N] dCSD78, 150 mM sodium chloride, 20 mM sodium phosphate, 1 mM DTT, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	dCSD78	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM

2 3D HNCA

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	dCSD78	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM

3 3D HNCACB

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	dCSD78	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM

4 3D CBCA(CO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	dCSD78	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM

5 3D HN(CO)CA

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	dCSD78	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM

6 3D HCCH-TOCSY

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	dCSD78	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM

7 3D HBHA(CO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	dCSD78	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM

8 3D HNCO

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	dCSD78	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM

9 2D 1H-1H NOESY

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4, Details 0.5 mM [U-99% 13C; U-99% 15N] dCSD78, 150 mM sodium chloride, 20 mM sodium phosphate, 1 mM DTT, 100% D2O

#	Name	Isotope labeling	Concentration
5	dCSD78	[U-99% 13C; U-99% 15N]	0.5 mM
6	sodium chloride	natural abundance	150 mM
7	sodium phosphate	natural abundance	20 mM
8	DTT	natural abundance	1 mM

10 3D 1H-15N NOESY

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	dCSD78	[U-99% 13C; U-99% 15N]	0.5 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	20 mM
4	DTT	natural abundance	1 mM

11 3D 1H-13C NOESY

Instrument

Bruker AVANCE III - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
5	dCSD78	[U-99% 13C; U-99% 15N]	0.5 mM
6	sodium chloride	natural abundance	150 mM
7	sodium phosphate	natural abundance	20 mM
8	DTT	natural abundance	1 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34492_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34492_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1990		99.4 (chain: 1, length: 168)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 168, Sequence coverage: 99.4%
- Total number of assignments
  - ¹H chemical shifts: 1152
  - ¹³C chemical shifts: 666
  - ¹⁵N chemical shifts: 172
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
69	CYS	HG	0.79
103	CYS	HG	1.141
129	HIS	HD1	6.465
161	CYS	HG	0.808

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	999	956	95.7
¹³C chemical shifts	744	666	89.5
¹⁵N chemical shifts	193	172	89.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	343	333	97.1
¹³C chemical shifts	336	304	90.5
¹⁵N chemical shifts	161	154	95.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	656	623	95.0
¹³C chemical shifts	408	362	88.7
¹⁵N chemical shifts	32	18	56.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	98	96	98.0
¹³C chemical shifts	98	93	94.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	70	67	95.7
¹³C chemical shifts	69	35	50.7
¹⁵N chemical shifts	1	1	100.0

Keywords CSD, RNA BINDING PROTEIN, ncCSD

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Found 1 Document (0.639 seconds)

BMRB: 34492

Sample #1

Sample #2

Related entities 1. Upstream of N-ras, isoform A, : 1 : 12 entities Detail

Experiments performed 11 experiments Detail

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Chemical shift validation 3 contents Detail