Ca2+-free Calmodulin mutant N53I
ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MIIEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.4 % (1563 of 1673) | 93.0 % (807 of 868) | 94.0 % (609 of 648) | 93.6 % (147 of 157) |
Backbone | 98.5 % (871 of 884) | 98.0 % (299 of 305) | 98.4 % (426 of 433) | 100.0 % (146 of 146) |
Sidechain | 89.3 % (827 of 926) | 90.2 % (508 of 563) | 90.3 % (318 of 352) | 9.1 % (1 of 11) |
Aromatic | 78.0 % (78 of 100) | 94.0 % (47 of 50) | 62.0 % (31 of 50) | |
Methyl | 90.4 % (132 of 146) | 95.9 % (70 of 73) | 84.9 % (62 of 73) |
1. entity 1
ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MIIEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAKSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.1 K, pH 6.3, Details 1.9 mM [U-99% 13C; U-99% 15N] Calmodulin N53I, 20 mM HEPES, 100 mM potassium chloride, 1 mM EDTA, 2 mM sodium azide, 0.05 mM TSP, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Calmodulin N53I | [U-99% 13C; U-99% 15N] | 1.9 mM | |
2 | HEPES | natural abundance | 20 mM | |
3 | potassium chloride | natural abundance | 100 mM | |
4 | EDTA | natural abundance | 1 mM | |
5 | sodium azide | natural abundance | 2 mM | |
6 | TSP | natural abundance | 0.05 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.1 K, pH 6.3, Details 1.2 mM [U-99% 15N] Calmodulin N53I, 2 mM HEPES, 10 mM potassium chloride, 10 mM EDTA, 2 mM sodium azide, 0.05 mM TSP, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Calmodulin N53I | [U-99% 15N] | 1.2 mM | |
8 | HEPES | natural abundance | 2 mM | |
9 | potassium chloride | natural abundance | 10 mM | |
10 | EDTA | natural abundance | 10 mM | |
11 | sodium azide | natural abundance | 2 mM | |
12 | TSP | natural abundance | 0.05 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.1 K, pH 6.3, Details 1.2 mM [U-99% 13C; U-99% 15N] Calmodulin, 2 mM HEPES, 10 mM potassium chloride, 10 mM EDTA, 2 mM sodium azide, 0.05 mM TSP, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | Calmodulin | [U-99% 13C; U-99% 15N] | 1.2 mM | |
14 | HEPES | natural abundance | 2 mM | |
15 | potassium chloride | natural abundance | 10 mM | |
16 | EDTA | natural abundance | 10 mM | |
17 | sodium azide | natural abundance | 2 mM | |
18 | TSP | natural abundance | 0.05 mM |