BMRBj - BMREntryMaster

Found 1 Document (0.48 seconds)

BMRB: 34503

6YET

Second EH domain of AtEH1/Pan1

Authors

Yperman, K., Papageorgiou, A., Evangelidis, T., Van Damme, D., Tripsianes, K.

Assembly

Second EH domain of ATEH1/Pan1

Entity

1. Second EH domain of ATEH1/Pan1, entity 1 (polymer), 106 monomers, 12320.94 Da Detail

GGGNNQPPWP KMKPSDVQKY TKVFMEVDSD KDGKITGEQA RNLFLSWRLP REVLKHVWEL SDQDNDTMLS LREFCISLYL MERYREGRPL PTALPSSIMF DETLLS

2. Second EH domain of ATEH1/Pan1, entity CA (non-polymer), 40.078 Da

Total weight

12361.019 Da

Max. entity weight

12320.94 Da

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 90.7 %, Completeness (bb): 82.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.7 % (1170 of 1290)	97.9 % (663 of 677)	79.7 % (396 of 497)	95.7 % (111 of 116)
Backbone	82.9 % (514 of 620)	97.1 % (204 of 210)	68.6 % (214 of 312)	98.0 % (96 of 98)
Sidechain	98.1 % (755 of 770)	98.1 % (458 of 467)	98.9 % (282 of 285)	83.3 % (15 of 18)
Aromatic	94.2 % (98 of 104)	94.2 % (49 of 52)	93.9 % (46 of 49)	100.0 % (3 of 3)
Methyl	100.0 % (98 of 98)	100.0 % (49 of 49)	100.0 % (49 of 49)

1. entity 1

GGGNNQPPWP KMKPSDVQKY TKVFMEVDSD KDGKITGEQA RNLFLSWRLP REVLKHVWEL SDQDNDTMLS LREFCISLYL MERYREGRPL PTALPSSIMF DETLLS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] EH12, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	EH12	[U-99% 13C; U-99% 15N]		1 mM

Release date

2020-07-13

Citation

Distinct EH domains of the endocytic TPLATE complex confer lipid and protein binding
Yperman, K., Papageorgiou, A.C., Merceron, R., De Munck, S., Bloch, Y., Eeckhout, D., Jiang, Q., Tack, P., Grigoryan, R., Evangelidis, T., Van Leene, J., Vincze, L., Vandenabeele, P., Vanhaecke, F., Potocky, M., De Jaeger, G., Savvides, S.N., Tripsianes, K., Pleskot, R., Van Damme, D.
Nat. Commun. (2021), 12, 3050-3050, PubMed 34031427 , DOI 10.1038/s41467-021-23314-6 , Abstract

Related entities 1. Second EH domain of ATEH1/Pan1, entity 1, : 1 : 1 : 141 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34503_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34503_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1268		98.1 (chain: 1, length: 106)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 106, Sequence coverage: 98.1%
- Total number of assignments
  - ¹H chemical shifts: 772
  - ¹³C chemical shifts: 386
  - ¹⁵N chemical shifts: 110
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	677	664	98.1
¹³C chemical shifts	497	386	77.7
¹⁵N chemical shifts	116	110	94.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	210	203	96.7
¹³C chemical shifts	212	104	49.1
¹⁵N chemical shifts	98	95	96.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	467	461	98.7
¹³C chemical shifts	285	282	98.9
¹⁵N chemical shifts	18	15	83.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	54	54	100.0
¹³C chemical shifts	54	54	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	52	49	94.2
¹³C chemical shifts	49	46	93.9
¹⁵N chemical shifts	3	3	100.0

Keywords Adaptor protein, Calcium binding, EF-hand, EH domain, LIPID BINDING PROTEIN, lipid binding

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Found 1 Document (0.48 seconds)

BMRB: 34503

Sample

Related entities 1. Second EH domain of ATEH1/Pan1, entity 1, : 1 : 1 : 141 entities Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail