BMRBj - BMREntryMaster

Found 1 Document (0.751 seconds)

BMRB: 34504

6YEU

Second EH domain of AtEH1/Pan1

Authors

Yperman, K., Papageorgiou, A., Evangelidis, T., Van Damme, D., Tripsianes, K.

Assembly

Calcium-binding EF hand family protein

Entity

1. Calcium-binding EF hand family protein, entity 1 (polymer, Thiol state: not present), 109 monomers, 11724.02 Da Detail

GGMAGQNPNM DQFEAYFKRA DLDGDGRISG AEAVGFFQGS GLSKQVLAQI WSLSDRSHSG FLDRQNFYNS LRLVTVAQSK RDLTPEIVNA ALNTPAAAKI PPPKINLSA

2. Calcium-binding EF hand family protein, entity CA (non-polymer), 40.078 Da

Total weight

11764.098 Da

Max. entity weight

11724.02 Da

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 91.0 %, Completeness (bb): 84.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.0 % (1127 of 1238)	98.4 % (631 of 641)	79.9 % (378 of 473)	95.2 % (118 of 124)
Backbone	84.1 % (540 of 642)	97.3 % (216 of 222)	70.3 % (223 of 317)	98.1 % (101 of 103)
Sidechain	98.8 % (687 of 695)	99.0 % (415 of 419)	100.0 % (255 of 255)	81.0 % (17 of 21)
Aromatic	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (45 of 45)	100.0 % (1 of 1)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. entity 1

GGMAGQNPNM DQFEAYFKRA DLDGDGRISG AEAVGFFQGS GLSKQVLAQI WSLSDRSHSG FLDRQNFYNS LRLVTVAQSK RDLTPEIVNA ALNTPAAAKI PPPKINLSA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-99% 13C; U-99% 15N] EH11, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	EH11	[U-99% 13C; U-99% 15N]		1 mM

Release date

2020-07-13

Citation

Distinct EH domains of the endocytic TPLATE complex confer lipid and protein binding
Yperman, K., Papageorgiou, A.C., Merceron, R., De Munck, S., Bloch, Y., Eeckhout, D., Jiang, Q., Tack, P., Grigoryan, R., Evangelidis, T., Van Leene, J., Vincze, L., Vandenabeele, P., Vanhaecke, F., Potocky, M., De Jaeger, G., Savvides, S.N., Tripsianes, K., Pleskot, R., Van Damme, D.
Nat. Commun. (2021), 12, 3050-3050, PubMed 34031427 , DOI 10.1038/s41467-021-23314-6 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 34503 released on 2020-07-13
Title Second EH domain of AtEH1/Pan1

Related entities 1. Calcium-binding EF hand family protein, entity 1, : 1 : 1 : 1 : 59 entities Detail

Experiments performed 4 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34504_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34504_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1227		98.2 (chain: 1, length: 109)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 109, Sequence coverage: 98.2%
- Total number of assignments
  - ¹H chemical shifts: 747
  - ¹³C chemical shifts: 362
  - ¹⁵N chemical shifts: 118
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	641	631	98.4
¹³C chemical shifts	473	362	76.5
¹⁵N chemical shifts	124	118	95.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	222	216	97.3
¹³C chemical shifts	218	107	49.1
¹⁵N chemical shifts	103	101	98.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	419	415	99.0
¹³C chemical shifts	255	255	100.0
¹⁵N chemical shifts	21	17	81.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	58	58	100.0
¹³C chemical shifts	58	58	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	46	46	100.0
¹³C chemical shifts	45	45	100.0
¹⁵N chemical shifts	1	1	100.0

Keywords Adaptor protein, Calcium-binding, EF-hand, EH domain, PEPTIDE BINDING PROTEIN, peptide motif binding

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Found 1 Document (0.751 seconds)

BMRB: 34504

Sample

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Calcium-binding EF hand family protein, entity 1, : 1 : 1 : 1 : 59 entities Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail