BMRBj - BMREntryMaster

Found 1 Document (0.742 seconds)

BMRB: 34512

6YJL

Solution NMR structure of the C-terminal arm of RSV nucleoprotein

Authors

Cardone, C., Eleouet, J.F., Galloux, M., Sizun, C.

Assembly

Nucleoprotein

Entity

1. Nucleoprotein (polymer, Thiol state: not present), 33 monomers, 3624.955 Da Detail

GSGVINYSVL DLTAEELEAI KHQLNPKDND VEL

Formula weight

3624.955 Da

Source organism

Human respiratory syncytial virus A2

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.4 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.4 % (354 of 371)	96.3 % (184 of 191)	94.4 % (136 of 144)	94.4 % (34 of 36)
Backbone	96.4 % (189 of 196)	97.0 % (65 of 67)	96.9 % (94 of 97)	93.8 % (30 of 32)
Sidechain	95.1 % (196 of 206)	96.0 % (119 of 124)	93.6 % (73 of 78)	100.0 % (4 of 4)
Aromatic	100.0 % (12 of 12)	100.0 % (6 of 6)	100.0 % (6 of 6)
Methyl	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (23 of 23)

1. entity 1

GSGVINYSVL DLTAEELEAI KHQLNPKDND VEL

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±1) K, pH 6.5 (±0.2), Details 20 mM unlabeled sodium phosphate, 100 mM unlabeled sodium chloride, 1 mM unlabeled TCEP, 1 mM [U-13C; U-15N] RSV_N_Carm, 93% H2O/7% D2O

#	Name	Isotope labeling	Concentration
1	sodium phosphate	unlabeled	20 (±2.0) mM
2	sodium chloride	unlabeled	100 (±10.0) mM
3	TCEP	unlabeled	1 (±0.1) mM
4	RSV_N_Carm	[U-13C; U-15N]	1 (±0.1) mM

Release date

2020-04-05

Citation

Solution NMR structure of the C-terminal arm of RSV nucleoprotein
Cardone, C., Eleouet, J.F., Galloux, M., Sizun, C.

Related entities 1. Nucleoprotein, : 1 : 3 : 6 entities Detail

Interaction partners 1. Nucleoprotein, : 11 interactors Detail

More details for 11 interactors identified by 3 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±1) K, pH 6.5 (±0.2), Details 20 mM unlabeled sodium phosphate, 100 mM unlabeled sodium chloride, 1 mM unlabeled TCEP, 1 mM [U-13C; U-15N] RSV_N_Carm, 93% H2O/7% D2O

#	Name	Isotope labeling	Concentration
1	sodium phosphate	unlabeled	20 (±2.0) mM
2	sodium chloride	unlabeled	100 (±10.0) mM
3	TCEP	unlabeled	1 (±0.1) mM
4	RSV_N_Carm	[U-13C; U-15N]	1 (±0.1) mM

2 3D HNCO

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	unlabeled	20 (±2.0) mM
2	sodium chloride	unlabeled	100 (±10.0) mM
3	TCEP	unlabeled	1 (±0.1) mM
4	RSV_N_Carm	[U-13C; U-15N]	1 (±0.1) mM

3 3D HNCA

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	unlabeled	20 (±2.0) mM
2	sodium chloride	unlabeled	100 (±10.0) mM
3	TCEP	unlabeled	1 (±0.1) mM
4	RSV_N_Carm	[U-13C; U-15N]	1 (±0.1) mM

4 3D HN(CO)CA

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	unlabeled	20 (±2.0) mM
2	sodium chloride	unlabeled	100 (±10.0) mM
3	TCEP	unlabeled	1 (±0.1) mM
4	RSV_N_Carm	[U-13C; U-15N]	1 (±0.1) mM

5 3D HN(COCA)CB

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	unlabeled	20 (±2.0) mM
2	sodium chloride	unlabeled	100 (±10.0) mM
3	TCEP	unlabeled	1 (±0.1) mM
4	RSV_N_Carm	[U-13C; U-15N]	1 (±0.1) mM

6 3D HBHANH

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	unlabeled	20 (±2.0) mM
2	sodium chloride	unlabeled	100 (±10.0) mM
3	TCEP	unlabeled	1 (±0.1) mM
4	RSV_N_Carm	[U-13C; U-15N]	1 (±0.1) mM

7 3D HBHA(CO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	unlabeled	20 (±2.0) mM
2	sodium chloride	unlabeled	100 (±10.0) mM
3	TCEP	unlabeled	1 (±0.1) mM
4	RSV_N_Carm	[U-13C; U-15N]	1 (±0.1) mM

8 3D HNCACB

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	unlabeled	20 (±2.0) mM
2	sodium chloride	unlabeled	100 (±10.0) mM
3	TCEP	unlabeled	1 (±0.1) mM
4	RSV_N_Carm	[U-13C; U-15N]	1 (±0.1) mM

9 3D 1H-15N NOESY

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	unlabeled	20 (±2.0) mM
2	sodium chloride	unlabeled	100 (±10.0) mM
3	TCEP	unlabeled	1 (±0.1) mM
4	RSV_N_Carm	[U-13C; U-15N]	1 (±0.1) mM

10 2D 1H-13C HSQC

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	unlabeled	20 (±2.0) mM
2	sodium chloride	unlabeled	100 (±10.0) mM
3	TCEP	unlabeled	1 (±0.1) mM
4	RSV_N_Carm	[U-13C; U-15N]	1 (±0.1) mM

11 3D H(CCO)NH

Instrument

Bruker AVANCE III - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	sodium phosphate	unlabeled	20 (±2.0) mM
2	sodium chloride	unlabeled	100 (±10.0) mM
3	TCEP	unlabeled	1 (±0.1) mM
4	RSV_N_Carm	[U-13C; U-15N]	1 (±0.1) mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34512_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34512_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	400		100.0 (chain: 1, length: 33)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 33, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 230
  - ¹³C chemical shifts: 136
  - ¹⁵N chemical shifts: 34
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	191	184	96.3
¹³C chemical shifts	144	136	94.4
¹⁵N chemical shifts	36	34	94.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	67	65	97.0
¹³C chemical shifts	66	63	95.5
¹⁵N chemical shifts	32	30	93.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	124	119	96.0
¹³C chemical shifts	78	73	93.6
¹⁵N chemical shifts	4	4	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	23	23	100.0
¹³C chemical shifts	23	23	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	6	6	100.0
¹³C chemical shifts	6	6	100.0

Keywords CYANA NMR Respiratory Syncytial Virus Nucleoprotein Nucleocapsid RNA dependent RNA polymerase Peptide, Intrinsically disordered region, VIRAL PROTEIN

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.742 seconds)

BMRB: 34512

Sample

Related entities 1. Nucleoprotein, : 1 : 3 : 6 entities Detail

Interaction partners 1. Nucleoprotein, : 11 interactors Detail

Experiments performed 11 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail