BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	81.4 % (1122 of 1379)	89.5 % (640 of 715)	66.2 % (354 of 535)	99.2 % (128 of 129)
Backbone	95.6 % (648 of 678)	97.4 % (227 of 233)	93.2 % (315 of 338)	99.1 % (106 of 107)
Sidechain	70.6 % (570 of 807)	85.7 % (413 of 482)	44.6 % (135 of 303)	100.0 % (22 of 22)
Aromatic	45.5 % (71 of 156)	87.2 % (68 of 78)	2.6 % (2 of 77)	100.0 % (1 of 1)
Methyl	74.6 % (85 of 114)	100.0 % (57 of 57)	49.1 % (28 of 57)

1. entity 1

GSGYTNYNNG SNYTQKKQTI GLPPQVNPQA VDHIIRSAPP RVTTAYIGNI PHFATEADLI PLFQNFGFIL DFKHYPEKGC CFIKYDTHEQ AAVCIVALAN FPFQGRNLRT GWGKER

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 301.2 K, pH 5.0, Details 250 uM eIF4G1(37-49)/PUB1(RRM3) Chimera, 25 mM potassium phosphate, 25 mM sodium chloride, 0.1 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	eIF4G1(37-49)/PUB1(RRM3) Chimera	natural abundance	250 uM
2	potassium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	25 mM
4	DTT	natural abundance	0.1 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 301.2 K, pH 5.0, Details 250 uM eIF4G1(37-49)/PUB1(RRM3) Chimera, 25 mM potassium phosphate, 25 mM sodium chloride, 0.1 mM DTT, 100% D2O

#	Name	Isotope labeling	Concentration
5	eIF4G1(37-49)/PUB1(RRM3) Chimera	natural abundance	250 uM
6	potassium phosphate	natural abundance	25 mM
7	sodium chloride	natural abundance	25 mM
8	DTT	natural abundance	0.1 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 301.2 K, pH 5.0, Details 148 uM [U-13C; U-15N] eIF4G1(37-49)/PUB1(RRM3) Chimera, 25 mM potassium phosphate, 25 mM sodium chloride, 0.1 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
9	eIF4G1(37-49)/PUB1(RRM3) Chimera	[U-13C; U-15N]	148 uM
10	potassium phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	25 mM
12	DTT	natural abundance	0.1 mM

Release date

2020-07-13

Citation

eIF4G1 N-terminal intrinsically disordered domain is a multi-docking station for RNA, Pab1, Pub1, and self-assembly
Chaves-Arquero, B., Martinez-Lumbreras, S., Sibille, N., Camero, S., Bernardo, P., Jimenez, M.A., Zorrilla, S., Perez-Canadillas, J.M.
Front. Mol. Biosci. (2022), 9, 986121-986121, PubMed 36213119 , DOI 10.3389/fmolb.2022.986121 , Abstract

Related entities 1. Eukaryotic initiation factor 4F subunit p150,Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1, : 1 : 1 : 25 entities Detail

Interaction partners 1. Eukaryotic initiation factor 4F subunit p150,Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1, : 19 interactors Detail

More details for 19 interactors identified by 4 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 301.2 K, pH 5.0, Details 148 uM [U-13C; U-15N] eIF4G1(37-49)/PUB1(RRM3) Chimera, 25 mM potassium phosphate, 25 mM sodium chloride, 0.1 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
9	eIF4G1(37-49)/PUB1(RRM3) Chimera	[U-13C; U-15N]	148 uM
10	potassium phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	25 mM
12	DTT	natural abundance	0.1 mM

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
9	eIF4G1(37-49)/PUB1(RRM3) Chimera	[U-13C; U-15N]	148 uM
10	potassium phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	25 mM
12	DTT	natural abundance	0.1 mM

3 3D HNCO

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
9	eIF4G1(37-49)/PUB1(RRM3) Chimera	[U-13C; U-15N]	148 uM
10	potassium phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	25 mM
12	DTT	natural abundance	0.1 mM

4 3D HNCA

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
9	eIF4G1(37-49)/PUB1(RRM3) Chimera	[U-13C; U-15N]	148 uM
10	potassium phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	25 mM
12	DTT	natural abundance	0.1 mM

5 3D CBCA(CO)NH

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
9	eIF4G1(37-49)/PUB1(RRM3) Chimera	[U-13C; U-15N]	148 uM
10	potassium phosphate	natural abundance	25 mM
11	sodium chloride	natural abundance	25 mM
12	DTT	natural abundance	0.1 mM

6 2D 1H-1H NOESY

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 301.2 K, pH 5.0, Details 250 uM eIF4G1(37-49)/PUB1(RRM3) Chimera, 25 mM potassium phosphate, 25 mM sodium chloride, 0.1 mM DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	eIF4G1(37-49)/PUB1(RRM3) Chimera	natural abundance	250 uM
2	potassium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	25 mM
4	DTT	natural abundance	0.1 mM

7 2D 1H-1H NOESY

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 301.2 K, pH 5.0, Details 250 uM eIF4G1(37-49)/PUB1(RRM3) Chimera, 25 mM potassium phosphate, 25 mM sodium chloride, 0.1 mM DTT, 100% D2O

#	Name	Isotope labeling	Concentration
5	eIF4G1(37-49)/PUB1(RRM3) Chimera	natural abundance	250 uM
6	potassium phosphate	natural abundance	25 mM
7	sodium chloride	natural abundance	25 mM
8	DTT	natural abundance	0.1 mM

8 2D 1H-1H TOCSY

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	eIF4G1(37-49)/PUB1(RRM3) Chimera	natural abundance	250 uM
2	potassium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	25 mM
4	DTT	natural abundance	0.1 mM

9 2D 1H-1H TOCSY

Instrument

Bruker AVANCE II - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
5	eIF4G1(37-49)/PUB1(RRM3) Chimera	natural abundance	250 uM
6	potassium phosphate	natural abundance	25 mM
7	sodium chloride	natural abundance	25 mM
8	DTT	natural abundance	0.1 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34517_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34517_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1232		99.1 (chain: 1, length: 116)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 116, Sequence coverage: 99.1%
- Total number of assignments
  - ¹³C chemical shifts: 351
  - ¹H chemical shifts: 754
  - ¹⁵N chemical shifts: 127
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
36	ARG	CZ	159.599
41	ARG	CZ	159.565
85	TYR	HH	8.816
94	CYS	HG	1.171
106	ARG	CZ	159.534
109	ARG	CZ	159.565
110	THR	HG1	5.764
116	ARG	CZ	159.512

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	535	346	64.7
¹H chemical shifts	715	637	89.1
¹⁵N chemical shifts	129	127	98.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	232	218	94.0
¹H chemical shifts	233	222	95.3
¹⁵N chemical shifts	107	105	98.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	303	128	42.2
¹H chemical shifts	482	415	86.1
¹⁵N chemical shifts	22	22	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	57	28	49.1
¹H chemical shifts	57	57	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	77	0	0.0
¹H chemical shifts	78	68	87.2
¹⁵N chemical shifts	1	1	100.0

Keywords Stress Granules, TRANSLATION, Translation Regulation

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Found 1 Document (0.546 seconds)

BMRB: 34517

Sample #1

Sample #2

Sample #3

Related entities 1. Eukaryotic initiation factor 4F subunit p150,Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1, : 1 : 1 : 25 entities Detail

Interaction partners 1. Eukaryotic initiation factor 4F subunit p150,Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1, : 19 interactors Detail

Experiments performed 9 experiments Detail

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Chemical shift validation 3 contents Detail