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NMR structural analysis of yeast Cox13 reveals its C-terminus in interaction with ATP
Authors
Shu, Z., Pontus, P., Peter, B., Lena, M., Pia, A.
Assembly
Cytochrome c oxidase subunit 13, mitochondrial
Entity
1. Cytochrome c oxidase subunit 13, mitochondrial (polymer), 129 monomers, 15020.93 × 2 Da Detail

MFRQCAKRYA SSLPPNALKP AFGPPDKVAA QKFKESLMAT EKHAKDTSNM WVKISVWVAL PAIALTAVNT YFVEKEHAEH REHLKHVPDS EWPRDYEFMN IRSKPFFWGD GDKTLFWNPV VNRHIEHDD


Total weight
30041.86 Da
Max. entity weight
15020.93 Da
Source organism
Saccharomyces cerevisiae S288C
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 90.7 %, Completeness: 58.6 %, Completeness (bb): 85.5 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All58.6 % (924 of 1576)51.7 % (424 of 820)63.9 % (395 of 618)76.1 % (105 of 138)
Backbone85.5 % (645 of 754)83.3 % (209 of 251)86.2 % (331 of 384)88.2 % (105 of 119)
Sidechain41.4 % (392 of 948)38.0 % (216 of 569)48.9 % (176 of 360) 0.0 % (0 of 19)
Aromatic 9.9 % (19 of 192)19.8 % (19 of 96) 0.0 % (0 of 91) 0.0 % (0 of 5)
Methyl81.0 % (94 of 116)81.0 % (47 of 58)81.0 % (47 of 58)

1. entity 1

MFRQCAKRYA SSLPPNALKP AFGPPDKVAA QKFKESLMAT EKHAKDTSNM WVKISVWVAL PAIALTAVNT YFVEKEHAEH REHLKHVPDS EWPRDYEFMN IRSKPFFWGD GDKTLFWNPV VNRHIEHDD

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313.15 K, pH 6.5, Details 0.5 mM [U-15N] Cytochrome c oxidase subunit 13, Cox13, 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
1Cytochrome c oxidase subunit 13, Cox13[U-15N]0.5 mM
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313.15 K, pH 6.5, Details 0.5 mM [U-13C; U-15N] Cytochrome c oxidase subunit 13, Cox13, 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
2Cytochrome c oxidase subunit 13, Cox13[U-13C; U-15N]0.5 mM
Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313.15 K, pH 6.5, Details 0.5 mM 50%[15N, 13C] mixed with 50% [14N, 12C] Cytochrome c oxidase subunit 13, Cox13, 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
3Cytochrome c oxidase subunit 13, Cox1350%[15N, 13C] mixed with 50% [14N, 12C]0.5 mM

Release date
2020-07-13
Citation
NMR structural analysis of the yeast cytochrome c oxidase subunit Cox13 and its interaction with ATP
Zhou, S., Pettersson, P., Bjorck, M.L., Dawitz, H., Brzezinski, P., Maler, L., Adelroth, P.
BMC Biol. (2021), 19, 98-98, PubMed 33971868 , DOI 10.1186/s12915-021-01036-x ,
Related entities 1. Cytochrome c oxidase subunit 13, mitochondrial, : 1 : 5 : 6 : 6 entities Detail
Interaction partners 1. Cytochrome c oxidase subunit 13, mitochondrial, : 6 interactors Detail
Experiments performed 7 experiments Detail
Chemical shift validation 3 contents Detail
Keywords ATP/ADP, STRUCTURAL PROTEIN, membrane protein, solution structure