BMRBj - BMREntryMaster

Found 1 Document (0.304 seconds)

BMRB: 34525

Structure of a parallel c-Myc modified with 5' duplex stem-loop overhang

Authors

Vianney, Y.M., Weisz, K.

Assembly

DNA (35-MER)

Entity

1. DNA (35-MER) (polymer, Thiol state: not present), 35 monomers, 11049.01 Da Detail

GATCAGTTTT ACTGATCGGG TGGGTAGGGT GGGTA

Formula weight

11049.01 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 64.8 %, Completeness (bb): 56.7 % Detail

Polymer type: polydeoxyribonucleotide

	Total	¹H
All	64.8 % (215 of 332)	64.8 % (215 of 332)
Suger, PO4	56.7 % (139 of 245)	56.7 % (139 of 245)
Nucleobase	87.4 % (76 of 87)	87.4 % (76 of 87)
Aromatic	87.1 % (61 of 70)	87.1 % (61 of 70)
Methyl	100.0 % (11 of 11)	100.0 % (11 of 11)

1. entity 1

GATCAGTTTT ACTGATCGGG TGGGTAGGGT GGGTA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.0, Details 0.5 mM 0 Nucleic acid, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Nucleic acid	natural abundance		0.5 mM

Release date

2020-07-01

Citation

Quadruplex-Duplex Junction: A High-Affinity Binding Site for Indoloquinoline Ligands
Vianney, Y.M., Preckwinkel, P., Mohr, S., Weisz, K.
Chemistry (2020), 26, 16910-16922, PubMed 32975874 , DOI 10.1002/chem.202003540 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 34533 released on 2020-07-26
    Title Structure of a parallel c-myc modified with 5' duplex stem-loop and 3' diagonal snap-back loop
  BMRB: 34524 released on 2020-07-07
    Title Structure of a parallel c-Myc modified with 3' duplex stem-loop overhang

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	237		100.0 (chain: 1, length: 35)
1	Chemical shift references	chem_shift_reference_1	OK	1		No information

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	332	215	64.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	245	139	56.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	87	76	87.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	11	11	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	27	26	96.3

Keywords DNA, Duplex, G-quadruplex, Quadruplex-Duplex Junction

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34525_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

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Found 1 Document (0.304 seconds)

BMRB: 34525

Sample

Entries sharing articles BMRB: 2 entries Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail