BMRB: 34529

G-quadruplex with a G-A bulge

Authors

Lenarcic Zivkovic, M., Plavec, J.

Assembly

DNA (5'-D(*TP*GP*GP*GP*AP*GP*GP*GP*AP*GP*CP*GP*GP*GP*AP*GP*TP*GP*GP*G)-3')

Entity

1. DNA (5'-D(*TP*GP*GP*GP*AP*GP*GP*GP*AP*GP*CP*GP*GP*GP*AP*GP*TP*GP*GP*G)-3') (polymer, Thiol state: not present), 20 monomers, 6464.083 Da Detail

TGGGAGGGAG CGGGAGTGGG

Formula weight

6464.083 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.9 %, Completeness (bb): 95.0 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	92.9 % (171 of 184)	92.9 % (171 of 184)
Suger, PO4	95.0 % (133 of 140)	95.0 % (133 of 140)
Nucleobase	86.4 % (38 of 44)	86.4 % (38 of 44)
Aromatic	90.0 % (36 of 40)	90.0 % (36 of 40)
Methyl	100.0 % (2 of 2)	100.0 % (2 of 2)

1. entity 1

TGGGAGGGAG CGGGAGTGGG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.6 mM / DNA (5'-D(*TP*GP*GP*GP*AP*GP*GP*GP*AP*GP*CP*GP*GP*GP*AP*GP*TP*GP*GP*G)-3'), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*TP*GP*GP*GP*AP*GP*GP*GP*AP*GP*CP*GP*GP*GP*AP*GP*TP*GP*GP*G)-3')	natural abundance		0.6 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 0.6 mM / DNA (5'-D(*TP*GP*GP*GP*AP*GP*GP*GP*AP*GP*CP*GP*GP*GP*AP*GP*TP*GP*GP*G)-3'), 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	DNA (5'-D(*TP*GP*GP*GP*AP*GP*GP*GP*AP*GP*CP*GP*GP*GP*AP*GP*TP*GP*GP*G)-3')	natural abundance		0.6 mM

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Release date

2020-08-05

Citation

Structure of a DNA G-Quadruplex Related to Osteoporosis with a G-A Bulge Forming a Pseudo-loop
Lenarcic Zivkovic, M., Rozman, J., Plavec, J.
Molecules (2020), 25, 4867-4867, PubMed 33096904 , DOI 10.3390/molecules25204867 , Abstract

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail